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Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

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Related in: MedlinePlus

Cases of Buruli ulcer epidemiologically linked to Point Lonsdale, Australia, byresident/visitor status, age, and sex. Dashed lines are medians.
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Related In: Results  -  Collection


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Figure 5: Cases of Buruli ulcer epidemiologically linked to Point Lonsdale, Australia, byresident/visitor status, age, and sex. Dashed lines are medians.

Mentions: Most case-patients were adults and many were elderly (Figure 5), although 14 of the 79 were children <18years of age. Among visitors, there was a bimodal age distribution, with relatively lownumbers of adults 20–50 years of age. An estimate of the age-specific attack ratefor residents of Point Lonsdale/Queenscliff was obtained with reference to the 2001Australian census. Because census data were not available for the 2 towns separately, thecalculation assumes that the age distribution of Point Lonsdale and Queenscliff issimilar. A similar analysis for visitors was not performed because appropriatedenominators could not be determined. The risk appeared to increase strongly with age andwas ≈7× higher for those >55 years of age than inthose <55 years of age (p<0.001) (Figure6).


Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Cases of Buruli ulcer epidemiologically linked to Point Lonsdale, Australia, byresident/visitor status, age, and sex. Dashed lines are medians.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375796&req=5

Figure 5: Cases of Buruli ulcer epidemiologically linked to Point Lonsdale, Australia, byresident/visitor status, age, and sex. Dashed lines are medians.
Mentions: Most case-patients were adults and many were elderly (Figure 5), although 14 of the 79 were children <18years of age. Among visitors, there was a bimodal age distribution, with relatively lownumbers of adults 20–50 years of age. An estimate of the age-specific attack ratefor residents of Point Lonsdale/Queenscliff was obtained with reference to the 2001Australian census. Because census data were not available for the 2 towns separately, thecalculation assumes that the age distribution of Point Lonsdale and Queenscliff issimilar. A similar analysis for visitors was not performed because appropriatedenominators could not be determined. The risk appeared to increase strongly with age andwas ≈7× higher for those >55 years of age than inthose <55 years of age (p<0.001) (Figure6).

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

Show MeSH
Related in: MedlinePlus