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Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

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Related in: MedlinePlus

Map of Point Lonsdale/Queenscliff, Australia (postcode 3225), showing location ofhouses of affected permanent residents, mosquito traps, and other features mentionedin the text. Not all traps yielded PCR-positive mosquitoes during the trapping period.Neg, negative; pos, positive.
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Figure 3: Map of Point Lonsdale/Queenscliff, Australia (postcode 3225), showing location ofhouses of affected permanent residents, mosquito traps, and other features mentionedin the text. Not all traps yielded PCR-positive mosquitoes during the trapping period.Neg, negative; pos, positive.

Mentions: The climate in Point Lonsdale is temperate with a mean daily maximum temperature of12.8°C in July (winter) and 22.4°C in January (summer). Average annualrainfall is 660 mm and is spread throughout the year (e.g., average 41.3 mm in January and59.1 mm in July) (27). Most of thetown is low-lying and close to sea level, and there are several natural and human-madeswamps and water features in the vicinity (Figure 3).Natural vegetation includes dense clumps of coastal tea trees (Leptospermumlaevigatum).


Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Map of Point Lonsdale/Queenscliff, Australia (postcode 3225), showing location ofhouses of affected permanent residents, mosquito traps, and other features mentionedin the text. Not all traps yielded PCR-positive mosquitoes during the trapping period.Neg, negative; pos, positive.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375796&req=5

Figure 3: Map of Point Lonsdale/Queenscliff, Australia (postcode 3225), showing location ofhouses of affected permanent residents, mosquito traps, and other features mentionedin the text. Not all traps yielded PCR-positive mosquitoes during the trapping period.Neg, negative; pos, positive.
Mentions: The climate in Point Lonsdale is temperate with a mean daily maximum temperature of12.8°C in July (winter) and 22.4°C in January (summer). Average annualrainfall is 660 mm and is spread throughout the year (e.g., average 41.3 mm in January and59.1 mm in July) (27). Most of thetown is low-lying and close to sea level, and there are several natural and human-madeswamps and water features in the vicinity (Figure 3).Natural vegetation includes dense clumps of coastal tea trees (Leptospermumlaevigatum).

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

Show MeSH
Related in: MedlinePlus