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Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

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Map of central coastal Victoria, Australia, showing towns and places referred to in thetext or in associated references.
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Figure 2: Map of central coastal Victoria, Australia, showing towns and places referred to in thetext or in associated references.

Mentions: In temperate southeastern Australia, outbreaks of M.ulcerans infection occur in localized areas, but few patients report directcontact with environmental water other than the ocean, which led to the proposal that aerosolsfrom contaminated water may cause human infections (13). However, these low-lying disease-endemic areas also harborlarge populations of mosquitoes, and some patients have reported that BU first appeared at thesite of what may have been a mosquito bite (Figure 1).These observations, and knowledge from field studies in Africa implicating insects as either areservoir or mode of transmission, led us to capture and screen mosquitoes during ourinvestigation of a large outbreak of BU in humans in a small coastal town in southeasternAustralia (Point Lonsdale), ≈60 km south of Melbourne (Figure 2).


Mycobacterium ulcerans in mosquitoes captured during outbreak of Buruli ulcer, southeastern Australia.

Johnson PD, Azuolas J, Lavender CJ, Wishart E, Stinear TP, Hayman JA, Brown L, Jenkin GA, Fyfe JA - Emerging Infect. Dis. (2007)

Map of central coastal Victoria, Australia, showing towns and places referred to in thetext or in associated references.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375796&req=5

Figure 2: Map of central coastal Victoria, Australia, showing towns and places referred to in thetext or in associated references.
Mentions: In temperate southeastern Australia, outbreaks of M.ulcerans infection occur in localized areas, but few patients report directcontact with environmental water other than the ocean, which led to the proposal that aerosolsfrom contaminated water may cause human infections (13). However, these low-lying disease-endemic areas also harborlarge populations of mosquitoes, and some patients have reported that BU first appeared at thesite of what may have been a mosquito bite (Figure 1).These observations, and knowledge from field studies in Africa implicating insects as either areservoir or mode of transmission, led us to capture and screen mosquitoes during ourinvestigation of a large outbreak of BU in humans in a small coastal town in southeasternAustralia (Point Lonsdale), ≈60 km south of Melbourne (Figure 2).

Bottom Line: BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007.We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus).Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

View Article: PubMed Central - PubMed

Affiliation: Infectious Disease Department, Austin Health, Melbourne, Victoria, Australia. paul.johnson@austin.org.au

ABSTRACT
Buruli ulcer (BU) occurs in >30 countries. The causative organism, Mycobacterium ulcerans, is acquired from the environment, but the exact mode of transmission is unknown. We investigated an outbreak of BU in a small coastal town in southeastern Australia and screened by PCR mosquitoes caught there. All cases of BU were confirmed by culture or PCR. Mosquitoes were trapped in multiple locations during a 26-month period. BU developed in 48 residents of Point Lonsdale/Queenscliff and 31 visitors from January 2001 through April 2007. We tested 11,504 mosquitoes trapped at Point Lonsdale (predominantly Aedes camptorhynchus). Forty-eight pools (5 species) were positive for insertion sequence IS2404 (maximum likelihood estimate 4.3/1,000), and we confirmed the presence of M. ulcerans in a subset of pools by detection of 3 additional PCR targets.

Show MeSH
Related in: MedlinePlus