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Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005.

Tu PV, Thao NT, Perera D, Huu TK, Tien NT, Thuong TC, How OM, Cardosa MJ, McMinn PC - Emerging Infect. Dis. (2007)

Bottom Line: HEV71 was isolated throughout the year, with a period of higher prevalence in October-November.Phylogenetic analysis of 23 HEV71 isolates showed that during the first half of 2005, viruses belonging to 3 subgenogroups, C1, C4, and a previously undescribed subgenogroup, C5, cocirculated in southern Vietnam.In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity.

View Article: PubMed Central - PubMed

Affiliation: Pasteur Institute, Ho Chi Minh City, Vietnam.

ABSTRACT
During 2005, 764 children were brought to a large children's hospital in Ho Chi Minh City, Vietnam, with a diagnosis of hand, foot, and mouth disease. All enrolled children had specimens (vesicle fluid, stool, throat swab) collected for enterovirus isolation by cell culture. An enterovirus was isolated from 411 (53.8%) of the specimens: 173 (42.1%) isolates were identified as human enterovirus 71 (HEV71) and 214 (52.1%) as coxsackievirus A16. Of the identified HEV71 infections, 51 (29.5%) were complicated by acute neurologic disease and 3 (1.7%) were fatal. HEV71 was isolated throughout the year, with a period of higher prevalence in October-November. Phylogenetic analysis of 23 HEV71 isolates showed that during the first half of 2005, viruses belonging to 3 subgenogroups, C1, C4, and a previously undescribed subgenogroup, C5, cocirculated in southern Vietnam. In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity.

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Monthly distribution of 387 cases of hand, foot, and mouth disease (HFMD) associated with isolation of either coxsackievirus A16 (CVA16) (214 cases) or human enterovirus 71 (HEV71) (173 cases), southern Vietnam, 2005. RNA was extracted from cells inoculated with vesicle, throat swab, or stool specimens. Partial VP4 gene sequences were amplified by reverse transcription–PCR (RT-PCR) by using specific primers (22), the amplified cDNA sequenced, and the serotype and/or genogroup specificity determined by BLAST analysis. A) Monthly distribution of CVA16 and HEV71-associated HFMD cases. B) Monthly distribution of 173 HFMD cases associated with HEV71 infection with strains belonging to subgenogroups C1, C4, or C5.
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Figure 3: Monthly distribution of 387 cases of hand, foot, and mouth disease (HFMD) associated with isolation of either coxsackievirus A16 (CVA16) (214 cases) or human enterovirus 71 (HEV71) (173 cases), southern Vietnam, 2005. RNA was extracted from cells inoculated with vesicle, throat swab, or stool specimens. Partial VP4 gene sequences were amplified by reverse transcription–PCR (RT-PCR) by using specific primers (22), the amplified cDNA sequenced, and the serotype and/or genogroup specificity determined by BLAST analysis. A) Monthly distribution of CVA16 and HEV71-associated HFMD cases. B) Monthly distribution of 173 HFMD cases associated with HEV71 infection with strains belonging to subgenogroups C1, C4, or C5.

Mentions: The distribution of CVA16- and HEV71-associated HFMD cases by month during 2005 is presented in Figure 3, panel A. HFMD was identified in southern Vietnam throughout the year; HEV71 and CVA16 were also isolated throughout the year. Two peaks of HFMD activity were observed during 2005. The first peak occurred from March through May. CVA16 was the predominant virus during this time, accounting for 81.1% (116 cases) of HFMD compared to 18.9% (27 cases) for HEV71 (Figure 2, panel A). The second peak of HFMD activity occurred from September through December. HEV71 was the predominant virus during this time, accounting for 65.3% (128 cases) of HFMD compared to 34.7% (68 cases) for CVA16 (Figure 3, panel A).


Epidemiologic and virologic investigation of hand, foot, and mouth disease, southern Vietnam, 2005.

Tu PV, Thao NT, Perera D, Huu TK, Tien NT, Thuong TC, How OM, Cardosa MJ, McMinn PC - Emerging Infect. Dis. (2007)

Monthly distribution of 387 cases of hand, foot, and mouth disease (HFMD) associated with isolation of either coxsackievirus A16 (CVA16) (214 cases) or human enterovirus 71 (HEV71) (173 cases), southern Vietnam, 2005. RNA was extracted from cells inoculated with vesicle, throat swab, or stool specimens. Partial VP4 gene sequences were amplified by reverse transcription–PCR (RT-PCR) by using specific primers (22), the amplified cDNA sequenced, and the serotype and/or genogroup specificity determined by BLAST analysis. A) Monthly distribution of CVA16 and HEV71-associated HFMD cases. B) Monthly distribution of 173 HFMD cases associated with HEV71 infection with strains belonging to subgenogroups C1, C4, or C5.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375788&req=5

Figure 3: Monthly distribution of 387 cases of hand, foot, and mouth disease (HFMD) associated with isolation of either coxsackievirus A16 (CVA16) (214 cases) or human enterovirus 71 (HEV71) (173 cases), southern Vietnam, 2005. RNA was extracted from cells inoculated with vesicle, throat swab, or stool specimens. Partial VP4 gene sequences were amplified by reverse transcription–PCR (RT-PCR) by using specific primers (22), the amplified cDNA sequenced, and the serotype and/or genogroup specificity determined by BLAST analysis. A) Monthly distribution of CVA16 and HEV71-associated HFMD cases. B) Monthly distribution of 173 HFMD cases associated with HEV71 infection with strains belonging to subgenogroups C1, C4, or C5.
Mentions: The distribution of CVA16- and HEV71-associated HFMD cases by month during 2005 is presented in Figure 3, panel A. HFMD was identified in southern Vietnam throughout the year; HEV71 and CVA16 were also isolated throughout the year. Two peaks of HFMD activity were observed during 2005. The first peak occurred from March through May. CVA16 was the predominant virus during this time, accounting for 81.1% (116 cases) of HFMD compared to 18.9% (27 cases) for HEV71 (Figure 2, panel A). The second peak of HFMD activity occurred from September through December. HEV71 was the predominant virus during this time, accounting for 65.3% (128 cases) of HFMD compared to 34.7% (68 cases) for CVA16 (Figure 3, panel A).

Bottom Line: HEV71 was isolated throughout the year, with a period of higher prevalence in October-November.Phylogenetic analysis of 23 HEV71 isolates showed that during the first half of 2005, viruses belonging to 3 subgenogroups, C1, C4, and a previously undescribed subgenogroup, C5, cocirculated in southern Vietnam.In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity.

View Article: PubMed Central - PubMed

Affiliation: Pasteur Institute, Ho Chi Minh City, Vietnam.

ABSTRACT
During 2005, 764 children were brought to a large children's hospital in Ho Chi Minh City, Vietnam, with a diagnosis of hand, foot, and mouth disease. All enrolled children had specimens (vesicle fluid, stool, throat swab) collected for enterovirus isolation by cell culture. An enterovirus was isolated from 411 (53.8%) of the specimens: 173 (42.1%) isolates were identified as human enterovirus 71 (HEV71) and 214 (52.1%) as coxsackievirus A16. Of the identified HEV71 infections, 51 (29.5%) were complicated by acute neurologic disease and 3 (1.7%) were fatal. HEV71 was isolated throughout the year, with a period of higher prevalence in October-November. Phylogenetic analysis of 23 HEV71 isolates showed that during the first half of 2005, viruses belonging to 3 subgenogroups, C1, C4, and a previously undescribed subgenogroup, C5, cocirculated in southern Vietnam. In the second half of the year, viruses belonging to subgenogroup C5 predominated during a period of higher HEV71 activity.

Show MeSH
Related in: MedlinePlus