Limits...
Panton-Valentine leukocidin genes in Staphylococcus aureus.

Melles DC, van Leeuwen WB, Boelens HA, Peeters JK, Verbrugh HA, van Belkum A - Emerging Infect. Dis. (2006)

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

The frequent recovery of staphylococcal isolates that produce leukocidal toxins from patients with deep skin and soft tissue infections, particularly furunculosis, cutaneous abscesses, and severe necrotizing pneumonia, suggests that the Panton-Valentine leukocidin (PVL) is 1 such virulence factor that has a major role in pathogenicity... PVL is mostly associated with community-acquired methicillin-resistant S. aureus (MRSA) infections and distinguishable from nosocomial MRSA by nonmultidrug resistance and carriage of the type IV staphylococcal chromosome cassette element (SCCmec type IV)... Five PVL-positive S. aureus strains (0.6%) were found in the carriage group (n = 829), and 3 (2.1%) of 146 blood-culture isolates carried the PVL gene (Table)... This finding is in agreement with previously reported low PVL prevalences by Prevost et al. (0% in 31 carriage isolates and 1.4% in 69 blood-culture isolates) and Von Eiff et al. (1.4% in 210 carriage isolates and 0.9% in 219 blood-culture isolates)... However, a higher prevalence of PVL (38.9%) was found in S. aureus strains causing abscesses and arthritis (Fisher exact test, p <0.0001)... No significant differences were found in the presence of PVL when carriage isolates were compared with invasive blood-culture isolates... PVL was found in each major genomic amplified fragment length polymorphism (AFLP) cluster, indicating that PVL has been introduced in distinct phylogenetic subpopulations of S. aureus (Figure)... Multilocus sequence typing analysis of a subset of the strain collection showed that the 15 PVL-positive strains were within clonal complex (CC) 30 (n = 7), CC 121 (n = 3), CC 1 (n = 2), CC 8 (n = 1), CC 22 (n = 1), and CC 45 (n = 1) (Table)... Although PVL was found among several staphylococcal genotypes, it was slightly overrepresented in AFLP cluster IVb (CC 121) compared with major clusters I and III... Whether the prevalence of PVL in carriage- and blood-culture isolates is higher and differs among distinct genetic clusters of S. aureus in countries with endemic CA-MRSA has to be investigated further... In conclusion, we have shown that the PVL-encoding phage has entered distinct staphylococcal lineages, although its prevalence differs per clonal group... PVL is associated with skin and soft tissue infections but not with bacteremia, which suggests that PVL is not likely to be involved in the pathogenesis of bacteremia.

Show MeSH

Related in: MedlinePlus

Principal component analysis of amplified fragment length polymorphism (AFLP) data of 1,033 Staphylococcus aureus strains. The different cubes (plotted in 3-dimensional space) represent every strain in the study. Each axis represents the score calculated for that strain on each principle component. The 4 circles indicate the different genetic AFLP clusters. Cluster IV could be subdivided in 2 minor clusters (8). The percentages (below each cluster) indicate the relative number of Panton-Valentine leukocidin (PVL)-positive strains in each major AFLP cluster. The single PVL-positive strain in cluster III is not visible behind the gray cubes. Gray, PVL-gene negative; red, PVL-gene positive.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3375734&req=5

Figure 1: Principal component analysis of amplified fragment length polymorphism (AFLP) data of 1,033 Staphylococcus aureus strains. The different cubes (plotted in 3-dimensional space) represent every strain in the study. Each axis represents the score calculated for that strain on each principle component. The 4 circles indicate the different genetic AFLP clusters. Cluster IV could be subdivided in 2 minor clusters (8). The percentages (below each cluster) indicate the relative number of Panton-Valentine leukocidin (PVL)-positive strains in each major AFLP cluster. The single PVL-positive strain in cluster III is not visible behind the gray cubes. Gray, PVL-gene negative; red, PVL-gene positive.

Mentions: Five PVL-positive S. aureus strains (0.6%) were found in the carriage group (n = 829), and 3 (2.1%) of 146 blood-culture isolates carried the PVL gene (Table). This finding is in agreement with previously reported low PVL prevalences by Prevost et al. (0% in 31 carriage isolates and 1.4% in 69 blood-culture isolates) and Von Eiff et al. (1.4% in 210 carriage isolates and 0.9% in 219 blood-culture isolates) (9,10). However, a higher prevalence of PVL (38.9%) was found in S. aureus strains causing abscesses and arthritis (Fisher exact test, p <0.0001) (8). This finding is also in agreement with the proposed involvement of PVL in severe and invasive (soft tissue) staphylococcal infections (1–3). No significant differences were found in the presence of PVL when carriage isolates were compared with invasive blood-culture isolates. PVL was found in each major genomic amplified fragment length polymorphism (AFLP) cluster, indicating that PVL has been introduced in distinct phylogenetic subpopulations of S. aureus (Figure). Multilocus sequence typing analysis of a subset of the strain collection showed that the 15 PVL-positive strains were within clonal complex (CC) 30 (n = 7), CC 121 (n = 3), CC 1 (n = 2), CC 8 (n = 1), CC 22 (n = 1), and CC 45 (n = 1) (Table) (8). Although PVL was found among several staphylococcal genotypes, it was slightly overrepresented in AFLP cluster IVb (CC 121) compared with major clusters I and III. Whether the prevalence of PVL in carriage- and blood-culture isolates is higher and differs among distinct genetic clusters of S. aureus in countries with endemic CA-MRSA has to be investigated further.


Panton-Valentine leukocidin genes in Staphylococcus aureus.

Melles DC, van Leeuwen WB, Boelens HA, Peeters JK, Verbrugh HA, van Belkum A - Emerging Infect. Dis. (2006)

Principal component analysis of amplified fragment length polymorphism (AFLP) data of 1,033 Staphylococcus aureus strains. The different cubes (plotted in 3-dimensional space) represent every strain in the study. Each axis represents the score calculated for that strain on each principle component. The 4 circles indicate the different genetic AFLP clusters. Cluster IV could be subdivided in 2 minor clusters (8). The percentages (below each cluster) indicate the relative number of Panton-Valentine leukocidin (PVL)-positive strains in each major AFLP cluster. The single PVL-positive strain in cluster III is not visible behind the gray cubes. Gray, PVL-gene negative; red, PVL-gene positive.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375734&req=5

Figure 1: Principal component analysis of amplified fragment length polymorphism (AFLP) data of 1,033 Staphylococcus aureus strains. The different cubes (plotted in 3-dimensional space) represent every strain in the study. Each axis represents the score calculated for that strain on each principle component. The 4 circles indicate the different genetic AFLP clusters. Cluster IV could be subdivided in 2 minor clusters (8). The percentages (below each cluster) indicate the relative number of Panton-Valentine leukocidin (PVL)-positive strains in each major AFLP cluster. The single PVL-positive strain in cluster III is not visible behind the gray cubes. Gray, PVL-gene negative; red, PVL-gene positive.
Mentions: Five PVL-positive S. aureus strains (0.6%) were found in the carriage group (n = 829), and 3 (2.1%) of 146 blood-culture isolates carried the PVL gene (Table). This finding is in agreement with previously reported low PVL prevalences by Prevost et al. (0% in 31 carriage isolates and 1.4% in 69 blood-culture isolates) and Von Eiff et al. (1.4% in 210 carriage isolates and 0.9% in 219 blood-culture isolates) (9,10). However, a higher prevalence of PVL (38.9%) was found in S. aureus strains causing abscesses and arthritis (Fisher exact test, p <0.0001) (8). This finding is also in agreement with the proposed involvement of PVL in severe and invasive (soft tissue) staphylococcal infections (1–3). No significant differences were found in the presence of PVL when carriage isolates were compared with invasive blood-culture isolates. PVL was found in each major genomic amplified fragment length polymorphism (AFLP) cluster, indicating that PVL has been introduced in distinct phylogenetic subpopulations of S. aureus (Figure). Multilocus sequence typing analysis of a subset of the strain collection showed that the 15 PVL-positive strains were within clonal complex (CC) 30 (n = 7), CC 121 (n = 3), CC 1 (n = 2), CC 8 (n = 1), CC 22 (n = 1), and CC 45 (n = 1) (Table) (8). Although PVL was found among several staphylococcal genotypes, it was slightly overrepresented in AFLP cluster IVb (CC 121) compared with major clusters I and III. Whether the prevalence of PVL in carriage- and blood-culture isolates is higher and differs among distinct genetic clusters of S. aureus in countries with endemic CA-MRSA has to be investigated further.

View Article: PubMed Central - PubMed

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

The frequent recovery of staphylococcal isolates that produce leukocidal toxins from patients with deep skin and soft tissue infections, particularly furunculosis, cutaneous abscesses, and severe necrotizing pneumonia, suggests that the Panton-Valentine leukocidin (PVL) is 1 such virulence factor that has a major role in pathogenicity... PVL is mostly associated with community-acquired methicillin-resistant S. aureus (MRSA) infections and distinguishable from nosocomial MRSA by nonmultidrug resistance and carriage of the type IV staphylococcal chromosome cassette element (SCCmec type IV)... Five PVL-positive S. aureus strains (0.6%) were found in the carriage group (n = 829), and 3 (2.1%) of 146 blood-culture isolates carried the PVL gene (Table)... This finding is in agreement with previously reported low PVL prevalences by Prevost et al. (0% in 31 carriage isolates and 1.4% in 69 blood-culture isolates) and Von Eiff et al. (1.4% in 210 carriage isolates and 0.9% in 219 blood-culture isolates)... However, a higher prevalence of PVL (38.9%) was found in S. aureus strains causing abscesses and arthritis (Fisher exact test, p <0.0001)... No significant differences were found in the presence of PVL when carriage isolates were compared with invasive blood-culture isolates... PVL was found in each major genomic amplified fragment length polymorphism (AFLP) cluster, indicating that PVL has been introduced in distinct phylogenetic subpopulations of S. aureus (Figure)... Multilocus sequence typing analysis of a subset of the strain collection showed that the 15 PVL-positive strains were within clonal complex (CC) 30 (n = 7), CC 121 (n = 3), CC 1 (n = 2), CC 8 (n = 1), CC 22 (n = 1), and CC 45 (n = 1) (Table)... Although PVL was found among several staphylococcal genotypes, it was slightly overrepresented in AFLP cluster IVb (CC 121) compared with major clusters I and III... Whether the prevalence of PVL in carriage- and blood-culture isolates is higher and differs among distinct genetic clusters of S. aureus in countries with endemic CA-MRSA has to be investigated further... In conclusion, we have shown that the PVL-encoding phage has entered distinct staphylococcal lineages, although its prevalence differs per clonal group... PVL is associated with skin and soft tissue infections but not with bacteremia, which suggests that PVL is not likely to be involved in the pathogenesis of bacteremia.

Show MeSH
Related in: MedlinePlus