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Orientia tsutsugamushi in eschars from scrub typhus patients.

Liu YX, Cao WC, Gao Y, Zhang JL, Yang ZQ, Zhao ZT, Foley JE - Emerging Infect. Dis. (2006)

Bottom Line: To verify the value of eschars for the diagnosis of scrub typhus and to characterize genotypes of Orientia tsutsugamushi in patients, we examined eschars and blood specimens of 7 patients from Shandong Province, People's Republic of China, for O. tsutsugamushi by polymerase chain reaction targeting the Sta56 gene.All 7 eschars and acute-phase blood samples were positive, while no specific DNA amplicons were obtained from the 7 convalescent-phase blood samples collected after antimicrobial drug therapy.The findings indicate that patients' eschars can be used for detection and genetic characterization of O. tsutsugamushi during the convalescent phase.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China.

ABSTRACT
To verify the value of eschars for the diagnosis of scrub typhus and to characterize genotypes of Orientia tsutsugamushi in patients, we examined eschars and blood specimens of 7 patients from Shandong Province, People's Republic of China, for O. tsutsugamushi by polymerase chain reaction targeting the Sta56 gene. All 7 eschars and acute-phase blood samples were positive, while no specific DNA amplicons were obtained from the 7 convalescent-phase blood samples collected after antimicrobial drug therapy. The findings indicate that patients' eschars can be used for detection and genetic characterization of O. tsutsugamushi during the convalescent phase.

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Related in: MedlinePlus

The locations of typical eschars in 2 representative patients with scrub typhus. A) An eschar on the neck of a patient (03PE1). B) An eschar on the waist of a patient (04PE5).
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Figure 1: The locations of typical eschars in 2 representative patients with scrub typhus. A) An eschar on the neck of a patient (03PE1). B) An eschar on the waist of a patient (04PE5).

Mentions: Seven scrub typhus patients reported at Feixian County (116°11´–118°18´E, 35°01´–35°33´N), Shandong Province, China, in September, October, and November of 2003–2004 were included in the study. The identification (ID) codes, age, sex, the locations of eschars, and other clinical characteristics on admission were documented (Table). The typical eschars of 2 patients (03PE1 and 04PE5) are shown in the Figure. After informed consent was obtained, 5 mL acute-phase blood was collected from each patient before treatment. Chloramphenicol was then administered orally at a dosage of 1.5–2.5 g 4×/day for 4–5 days. Fever resolved for all 7 patients within 2 days of treatment. Eschar specimens and 5-mL convalescent-phase blood sample from each patient were collected at the time that the eschar spontaneously desquamated (6–15 days after treatment). Serum specimens were separated by centrifugation at 2,500× g for 10 min. All specimens from eschars, serum, and residual blood clots were kept at -70°C until use.


Orientia tsutsugamushi in eschars from scrub typhus patients.

Liu YX, Cao WC, Gao Y, Zhang JL, Yang ZQ, Zhao ZT, Foley JE - Emerging Infect. Dis. (2006)

The locations of typical eschars in 2 representative patients with scrub typhus. A) An eschar on the neck of a patient (03PE1). B) An eschar on the waist of a patient (04PE5).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375733&req=5

Figure 1: The locations of typical eschars in 2 representative patients with scrub typhus. A) An eschar on the neck of a patient (03PE1). B) An eschar on the waist of a patient (04PE5).
Mentions: Seven scrub typhus patients reported at Feixian County (116°11´–118°18´E, 35°01´–35°33´N), Shandong Province, China, in September, October, and November of 2003–2004 were included in the study. The identification (ID) codes, age, sex, the locations of eschars, and other clinical characteristics on admission were documented (Table). The typical eschars of 2 patients (03PE1 and 04PE5) are shown in the Figure. After informed consent was obtained, 5 mL acute-phase blood was collected from each patient before treatment. Chloramphenicol was then administered orally at a dosage of 1.5–2.5 g 4×/day for 4–5 days. Fever resolved for all 7 patients within 2 days of treatment. Eschar specimens and 5-mL convalescent-phase blood sample from each patient were collected at the time that the eschar spontaneously desquamated (6–15 days after treatment). Serum specimens were separated by centrifugation at 2,500× g for 10 min. All specimens from eschars, serum, and residual blood clots were kept at -70°C until use.

Bottom Line: To verify the value of eschars for the diagnosis of scrub typhus and to characterize genotypes of Orientia tsutsugamushi in patients, we examined eschars and blood specimens of 7 patients from Shandong Province, People's Republic of China, for O. tsutsugamushi by polymerase chain reaction targeting the Sta56 gene.All 7 eschars and acute-phase blood samples were positive, while no specific DNA amplicons were obtained from the 7 convalescent-phase blood samples collected after antimicrobial drug therapy.The findings indicate that patients' eschars can be used for detection and genetic characterization of O. tsutsugamushi during the convalescent phase.

View Article: PubMed Central - PubMed

Affiliation: Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China.

ABSTRACT
To verify the value of eschars for the diagnosis of scrub typhus and to characterize genotypes of Orientia tsutsugamushi in patients, we examined eschars and blood specimens of 7 patients from Shandong Province, People's Republic of China, for O. tsutsugamushi by polymerase chain reaction targeting the Sta56 gene. All 7 eschars and acute-phase blood samples were positive, while no specific DNA amplicons were obtained from the 7 convalescent-phase blood samples collected after antimicrobial drug therapy. The findings indicate that patients' eschars can be used for detection and genetic characterization of O. tsutsugamushi during the convalescent phase.

Show MeSH
Related in: MedlinePlus