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A Study on the Expression of BCR-ABL Transcript in Mixed Phenotype Acute Leukemia (MPAL) Cases Using the Reverse Transcriptase Polymerase Reaction Assay (RT-PCR) and its Correlation with Hematological Remission Status Post Initial Induction Therapy.

Bhatia P, Binota J, Varma N, Bansal D, Trehan A, Marwaha RK, Malhotra P, Varma S - Mediterr J Hematol Infect Dis (2012)

Bottom Line: The single pediatric case was positive for b3a2 transcript.The positive cases also showed hematological remission at post induction check marrow (blasts<5%).This could partly be explained due to good response to the imatinib added to the treatment protocol.

View Article: PubMed Central - PubMed

Affiliation: Assistant Professor-Pediatrics.

ABSTRACT

Introduction: The MPAL comprise 2-5% of all acute leukemia. The present WHO 2008 classification has separated two groups in MPAL based on t(9;22) positivity and MLL rearrangement. AIMS #ENTITYSTARTX00026;

Objectives: The aim of the present pilot study is to note the frequency of BCR-ABL transcript in MPAL cases using the RT-PCR assay and to correlate the status with hematological remission post induction. MATERIALS #ENTITYSTARTX00026;

Methods: A total of 10 MPAL cases classified on Flow-cytometry based on the current WHO 2008 criteria were enrolled. In all the cases Bone marrow or peripheral blood sample in EDTA was processed for molecular studies and the RT-PCR reaction carried out using primers specific to the t (9;22) and t(4;11) translocation. The post induction check marrow slides were also reviewed.

Results: Out of the total 10 MPAL cases, 7/10 (70%) were adult and 3/10 (30%) pediatric cases. A total of 4/10 (40%) cases showed positivity for the t(9;22) transcript and none for t (4;11). Of the 4 positive cases, 3/10(30%) were adult cases and 1/10(10%) pediatric case. The BCR-ABL transcript type in adult cases was b3a2 (p210) in 2/3 (66%) and e1a2 (p190) in 1/3 (33.3%) case. The single pediatric case was positive for b3a2 transcript. DISCUSSION #ENTITYSTARTX00026;

Conclusion: All the 4 positive MPAL cases presented with high TLC and low platelet count (p<0.05). The positive cases also showed hematological remission at post induction check marrow (blasts<5%). This could partly be explained due to good response to the imatinib added to the treatment protocol.

No MeSH data available.


Related in: MedlinePlus

Geimsa Stain- PBF (1000X); Two population of Blasts. Arrow- Blast showing presence of Auer Rod like granules
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f3-mjhid-4-1-e2012024: Geimsa Stain- PBF (1000X); Two population of Blasts. Arrow- Blast showing presence of Auer Rod like granules

Mentions: In the present study all the MPAL cases positive for BCR-ABL transcript had high WBC count and low platelet count (p<0.05). Many studies have also shown BCR-ABL positive MPAL’s presenting with high WBC count4,5, but present study also shows relation of BCR-ABL positivity with low platelet count. There were 4 cases of T/Myeloid MPAL,of which one showed BCR-ABL positivity (Figure 3). Mastutes et al6 in their study had also found BCR-ABL positivity in 13% (2/15) cases with T/Myeloid phenotype.


A Study on the Expression of BCR-ABL Transcript in Mixed Phenotype Acute Leukemia (MPAL) Cases Using the Reverse Transcriptase Polymerase Reaction Assay (RT-PCR) and its Correlation with Hematological Remission Status Post Initial Induction Therapy.

Bhatia P, Binota J, Varma N, Bansal D, Trehan A, Marwaha RK, Malhotra P, Varma S - Mediterr J Hematol Infect Dis (2012)

Geimsa Stain- PBF (1000X); Two population of Blasts. Arrow- Blast showing presence of Auer Rod like granules
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375663&req=5

f3-mjhid-4-1-e2012024: Geimsa Stain- PBF (1000X); Two population of Blasts. Arrow- Blast showing presence of Auer Rod like granules
Mentions: In the present study all the MPAL cases positive for BCR-ABL transcript had high WBC count and low platelet count (p<0.05). Many studies have also shown BCR-ABL positive MPAL’s presenting with high WBC count4,5, but present study also shows relation of BCR-ABL positivity with low platelet count. There were 4 cases of T/Myeloid MPAL,of which one showed BCR-ABL positivity (Figure 3). Mastutes et al6 in their study had also found BCR-ABL positivity in 13% (2/15) cases with T/Myeloid phenotype.

Bottom Line: The single pediatric case was positive for b3a2 transcript.The positive cases also showed hematological remission at post induction check marrow (blasts<5%).This could partly be explained due to good response to the imatinib added to the treatment protocol.

View Article: PubMed Central - PubMed

Affiliation: Assistant Professor-Pediatrics.

ABSTRACT

Introduction: The MPAL comprise 2-5% of all acute leukemia. The present WHO 2008 classification has separated two groups in MPAL based on t(9;22) positivity and MLL rearrangement. AIMS #ENTITYSTARTX00026;

Objectives: The aim of the present pilot study is to note the frequency of BCR-ABL transcript in MPAL cases using the RT-PCR assay and to correlate the status with hematological remission post induction. MATERIALS #ENTITYSTARTX00026;

Methods: A total of 10 MPAL cases classified on Flow-cytometry based on the current WHO 2008 criteria were enrolled. In all the cases Bone marrow or peripheral blood sample in EDTA was processed for molecular studies and the RT-PCR reaction carried out using primers specific to the t (9;22) and t(4;11) translocation. The post induction check marrow slides were also reviewed.

Results: Out of the total 10 MPAL cases, 7/10 (70%) were adult and 3/10 (30%) pediatric cases. A total of 4/10 (40%) cases showed positivity for the t(9;22) transcript and none for t (4;11). Of the 4 positive cases, 3/10(30%) were adult cases and 1/10(10%) pediatric case. The BCR-ABL transcript type in adult cases was b3a2 (p210) in 2/3 (66%) and e1a2 (p190) in 1/3 (33.3%) case. The single pediatric case was positive for b3a2 transcript. DISCUSSION #ENTITYSTARTX00026;

Conclusion: All the 4 positive MPAL cases presented with high TLC and low platelet count (p<0.05). The positive cases also showed hematological remission at post induction check marrow (blasts<5%). This could partly be explained due to good response to the imatinib added to the treatment protocol.

No MeSH data available.


Related in: MedlinePlus