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Electron microscopy of the separated outer tegument of the sparganum and its antigenicity.

Yang HJ - Korean J. Parasitol. (2012)

Bottom Line: It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum.Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument.The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Ewha Global Challenge, Ewha Womans University School of Medicine, Seoul 158-710, Korea.

ABSTRACT
The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

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Related in: MedlinePlus

Whole sectional view of the tegument of a sparganum worm. The tegument consisted of a plasma membrane, including the spear-shaped microtriches (mt), protoplasmic layer, basement layer (BL), and muscle layer (ML). The rectangular area in (A) was processed for electron microscopic analysis. Note the asterisk (*) which means the separation of the basement layer (D) from the worm parenchyme (E). Magnification at ×40 (A), ×10,000 (B), ×12,000 (C), ×4,000 (D), and ×20,000 (E). Scale bars: 2 µm in (B), (C), and (E), 10 µm in (D).
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Figure 1: Whole sectional view of the tegument of a sparganum worm. The tegument consisted of a plasma membrane, including the spear-shaped microtriches (mt), protoplasmic layer, basement layer (BL), and muscle layer (ML). The rectangular area in (A) was processed for electron microscopic analysis. Note the asterisk (*) which means the separation of the basement layer (D) from the worm parenchyme (E). Magnification at ×40 (A), ×10,000 (B), ×12,000 (C), ×4,000 (D), and ×20,000 (E). Scale bars: 2 µm in (B), (C), and (E), 10 µm in (D).

Mentions: As shown in Fig. 1A, the toluidine blue-stained sparganum was clearly seen by light microscopy and also the tegumental separation was noticed almost around the sparganum. The rectangular area was processed for TEM analysis. As shown in Fig. 1B, non-treated control, the tegument of the sparganum was seen, and there was normal architecture of spear-shaped microtriches (mt) which were consistent with the results of the anterior portion of the worm [5], basal layer (BL), and muscle layer (ML). After the tegumental separation using urea solution, microtriches seemed to maintain their normal structures (Fig. 1C), and the basal layer was separated clearly (* in Fig. 1D, E). The sparganum had severely wrinkled folds on the tegument, and some folding portions seemed to be still attached to the parenchyme of the sparganum (Fig. 1A). However, by electron microscopy, it has been confirmed that the separation of the sparganum tegument by urea was clearly complete (Fig. 1D, E).


Electron microscopy of the separated outer tegument of the sparganum and its antigenicity.

Yang HJ - Korean J. Parasitol. (2012)

Whole sectional view of the tegument of a sparganum worm. The tegument consisted of a plasma membrane, including the spear-shaped microtriches (mt), protoplasmic layer, basement layer (BL), and muscle layer (ML). The rectangular area in (A) was processed for electron microscopic analysis. Note the asterisk (*) which means the separation of the basement layer (D) from the worm parenchyme (E). Magnification at ×40 (A), ×10,000 (B), ×12,000 (C), ×4,000 (D), and ×20,000 (E). Scale bars: 2 µm in (B), (C), and (E), 10 µm in (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375461&req=5

Figure 1: Whole sectional view of the tegument of a sparganum worm. The tegument consisted of a plasma membrane, including the spear-shaped microtriches (mt), protoplasmic layer, basement layer (BL), and muscle layer (ML). The rectangular area in (A) was processed for electron microscopic analysis. Note the asterisk (*) which means the separation of the basement layer (D) from the worm parenchyme (E). Magnification at ×40 (A), ×10,000 (B), ×12,000 (C), ×4,000 (D), and ×20,000 (E). Scale bars: 2 µm in (B), (C), and (E), 10 µm in (D).
Mentions: As shown in Fig. 1A, the toluidine blue-stained sparganum was clearly seen by light microscopy and also the tegumental separation was noticed almost around the sparganum. The rectangular area was processed for TEM analysis. As shown in Fig. 1B, non-treated control, the tegument of the sparganum was seen, and there was normal architecture of spear-shaped microtriches (mt) which were consistent with the results of the anterior portion of the worm [5], basal layer (BL), and muscle layer (ML). After the tegumental separation using urea solution, microtriches seemed to maintain their normal structures (Fig. 1C), and the basal layer was separated clearly (* in Fig. 1D, E). The sparganum had severely wrinkled folds on the tegument, and some folding portions seemed to be still attached to the parenchyme of the sparganum (Fig. 1A). However, by electron microscopy, it has been confirmed that the separation of the sparganum tegument by urea was clearly complete (Fig. 1D, E).

Bottom Line: It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum.Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument.The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology and Ewha Global Challenge, Ewha Womans University School of Medicine, Seoul 158-710, Korea.

ABSTRACT
The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.

Show MeSH
Related in: MedlinePlus