Limits...
Efficacy of ronidazole for treatment of cats experimentally infected with a Korean isolate of Tritrichomonas foetus.

Lim S, Park SI, Ahn KS, Oh DS, Shin SS - Korean J. Parasitol. (2012)

Bottom Line: Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks.To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay.The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.

ABSTRACT
To evaluate the efficacy of ronidazole for treatment of Tritrichomonas foetus infection, 6 Tritrichomonas-free kittens were experimentally infected with a Korean isolate of T. foetus. The experimental infection was confirmed by direct microscopy, culture, and single-tube nested PCR, and all cats demonstrated trophozoites of T. foetus by day 20 post-infection in the feces. From day 30 after the experimentally induced infection, 3 cats were treated with ronidazole (50 mg/kg twice a day for 14 days) and 3 other cats received placebo. Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks. To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay. All 3 cats in the treatment group receiving ronidazole showed negative results for T. foetus infection during 2 weeks of treatment and 4 weeks follow-up by all detection methods used in this study. In contrast, rectal swab samples from cats in the control group were positive for T. foetus continuously throughout the study. The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

Show MeSH

Related in: MedlinePlus

Single-tube nested PCR amplification (208-bp) of Tritrichomonas foetus with primers TFR3/TFR4 and primers TFITS-F/TFITS-R in cats from Korea on day 0 (A) and day 28 after the initiation of treatment with ronidazole at 50 mg/kg twice a day for 14 days (B). Lane 1, molecular weight marker; 2, positive control; 3, culture medium; 4, 5, 6, untreated control cats (C-16, C-70, C-71); 7, 8, 9, cats treated with ronidazole (C-30, C-45, C-77).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3375457&req=5

Figure 1: Single-tube nested PCR amplification (208-bp) of Tritrichomonas foetus with primers TFR3/TFR4 and primers TFITS-F/TFITS-R in cats from Korea on day 0 (A) and day 28 after the initiation of treatment with ronidazole at 50 mg/kg twice a day for 14 days (B). Lane 1, molecular weight marker; 2, positive control; 3, culture medium; 4, 5, 6, untreated control cats (C-16, C-70, C-71); 7, 8, 9, cats treated with ronidazole (C-30, C-45, C-77).

Mentions: All cats were confirmed to be free of T. foetus before experimentally induced infection by direct smear and single-tube nested PCR test of rectal swab samples (data not shown). Some of the cats started to reveal T. foetus trophozoites in rectal samples at day 7 after inoculation, and all cats were positive by day 20 PI (Table 1; Fig. 1A). No cat showed signs of diarrhea after experimentally induced infection in this study.


Efficacy of ronidazole for treatment of cats experimentally infected with a Korean isolate of Tritrichomonas foetus.

Lim S, Park SI, Ahn KS, Oh DS, Shin SS - Korean J. Parasitol. (2012)

Single-tube nested PCR amplification (208-bp) of Tritrichomonas foetus with primers TFR3/TFR4 and primers TFITS-F/TFITS-R in cats from Korea on day 0 (A) and day 28 after the initiation of treatment with ronidazole at 50 mg/kg twice a day for 14 days (B). Lane 1, molecular weight marker; 2, positive control; 3, culture medium; 4, 5, 6, untreated control cats (C-16, C-70, C-71); 7, 8, 9, cats treated with ronidazole (C-30, C-45, C-77).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375457&req=5

Figure 1: Single-tube nested PCR amplification (208-bp) of Tritrichomonas foetus with primers TFR3/TFR4 and primers TFITS-F/TFITS-R in cats from Korea on day 0 (A) and day 28 after the initiation of treatment with ronidazole at 50 mg/kg twice a day for 14 days (B). Lane 1, molecular weight marker; 2, positive control; 3, culture medium; 4, 5, 6, untreated control cats (C-16, C-70, C-71); 7, 8, 9, cats treated with ronidazole (C-30, C-45, C-77).
Mentions: All cats were confirmed to be free of T. foetus before experimentally induced infection by direct smear and single-tube nested PCR test of rectal swab samples (data not shown). Some of the cats started to reveal T. foetus trophozoites in rectal samples at day 7 after inoculation, and all cats were positive by day 20 PI (Table 1; Fig. 1A). No cat showed signs of diarrhea after experimentally induced infection in this study.

Bottom Line: Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks.To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay.The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea.

ABSTRACT
To evaluate the efficacy of ronidazole for treatment of Tritrichomonas foetus infection, 6 Tritrichomonas-free kittens were experimentally infected with a Korean isolate of T. foetus. The experimental infection was confirmed by direct microscopy, culture, and single-tube nested PCR, and all cats demonstrated trophozoites of T. foetus by day 20 post-infection in the feces. From day 30 after the experimentally induced infection, 3 cats were treated with ronidazole (50 mg/kg twice a day for 14 days) and 3 other cats received placebo. Feces from each cat were tested for the presence of T. foetus by direct smear and culture of rectal swab samples using modified Diamond's medium once a week for 4 weeks. To confirm the culture results, the presence of T. foetus rRNA gene was determined by single-tube nested PCR assay. All 3 cats in the treatment group receiving ronidazole showed negative results for T. foetus infection during 2 weeks of treatment and 4 weeks follow-up by all detection methods used in this study. In contrast, rectal swab samples from cats in the control group were positive for T. foetus continuously throughout the study. The present study indicates that ronidazole is also effective to treat cats infected experimentally with a Korean isolate of T. foetus at a dose of 50 mg/kg twice a day for 14 days.

Show MeSH
Related in: MedlinePlus