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Dirofilaria repens in Vietnam: detection of 10 eye and subcutaneous tissue infection cases identified by morphology and molecular methods.

De NV, Le TH, Chai JY - Korean J. Parasitol. (2012)

Bottom Line: Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification.A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced.Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814).

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam. ngvdeyhn@gmail.com

ABSTRACT
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.

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Dirofilaria repens collected from the conjunctiva (A, B) and subcutaneous tissue (C, D) of humans in Vietnam. (A) A female worm from the conjunctiva of a patient. (B) Another specimen from another patient. (C) Anterior end of a worm showing the mouth and esophagus (×100). (D) Posteror part of a female worm extracted from the subcutaneous tissue of a patient (×40).
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Figure 1: Dirofilaria repens collected from the conjunctiva (A, B) and subcutaneous tissue (C, D) of humans in Vietnam. (A) A female worm from the conjunctiva of a patient. (B) Another specimen from another patient. (C) Anterior end of a worm showing the mouth and esophagus (×100). (D) Posteror part of a female worm extracted from the subcutaneous tissue of a patient (×40).

Mentions: Parasites measured 4.0-12.5 cm in length and 0.5-0.6 mm in width. The worms were identified by the morphology, and pictures were taken (Fig. 1). Among the worms, 3 were chosen (2 from the conjunctiva and 1 from the subcutaneous tissue), marked as GCA-VN1, GCH-VN2, and GCD-VN3, respectively, and analyzed by molecular methods.


Dirofilaria repens in Vietnam: detection of 10 eye and subcutaneous tissue infection cases identified by morphology and molecular methods.

De NV, Le TH, Chai JY - Korean J. Parasitol. (2012)

Dirofilaria repens collected from the conjunctiva (A, B) and subcutaneous tissue (C, D) of humans in Vietnam. (A) A female worm from the conjunctiva of a patient. (B) Another specimen from another patient. (C) Anterior end of a worm showing the mouth and esophagus (×100). (D) Posteror part of a female worm extracted from the subcutaneous tissue of a patient (×40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375452&req=5

Figure 1: Dirofilaria repens collected from the conjunctiva (A, B) and subcutaneous tissue (C, D) of humans in Vietnam. (A) A female worm from the conjunctiva of a patient. (B) Another specimen from another patient. (C) Anterior end of a worm showing the mouth and esophagus (×100). (D) Posteror part of a female worm extracted from the subcutaneous tissue of a patient (×40).
Mentions: Parasites measured 4.0-12.5 cm in length and 0.5-0.6 mm in width. The worms were identified by the morphology, and pictures were taken (Fig. 1). Among the worms, 3 were chosen (2 from the conjunctiva and 1 from the subcutaneous tissue), marked as GCA-VN1, GCH-VN2, and GCD-VN3, respectively, and analyzed by molecular methods.

Bottom Line: Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification.A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced.Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814).

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Hanoi Medical University, Hanoi, Vietnam. ngvdeyhn@gmail.com

ABSTRACT
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.

Show MeSH
Related in: MedlinePlus