Limits...
Estrogen depletion results in nanoscale morphology changes in dermal collagen.

Fang M, Liroff KG, Turner AS, Les CM, Orr BG, Holl MM - J. Invest. Dermatol. (2012)

Bottom Line: In addition, the overall width of the distribution, both values above and below the mean, was found to be increased.The change in width due to an increase in lower values of D-spacings was previously reported for ovine bone; however, this report demonstrates that the effect is also present in non-mineralized collagen fibrils.A nonparametric Kolmogorov-Smirnov test of the cumulative density function indicates a statistical difference in the sham and OVX D-spacing distributions (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.

ABSTRACT
Tissue cryo-sectioning combined with atomic force microscopy imaging reveals that the nanoscale morphology of dermal collagen fibrils, quantified using the metric of D-periodic spacing, changes under the condition of estrogen depletion. Specifically, a new subpopulation of fibrils with D-spacings in the region between 56 and 59 nm is present 2 years following ovariectomy in ovine dermal samples. In addition, the overall width of the distribution, both values above and below the mean, was found to be increased. The change in width due to an increase in lower values of D-spacings was previously reported for ovine bone; however, this report demonstrates that the effect is also present in non-mineralized collagen fibrils. A nonparametric Kolmogorov-Smirnov test of the cumulative density function indicates a statistical difference in the sham and OVX D-spacing distributions (P<0.01).

Show MeSH

Related in: MedlinePlus

Sirius red staining reveals the abundance of fibrillar collagen content in Sham and OVX dermis. Panel a and b are polarized light microscopic images of Sham and OVX dermis, respectively. The magnification is 10×, dash lines represent epidermis. Panel c shows the collagen abundance measured from the staining intensity (*p <0.05).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3375339&req=5

Figure 4: Sirius red staining reveals the abundance of fibrillar collagen content in Sham and OVX dermis. Panel a and b are polarized light microscopic images of Sham and OVX dermis, respectively. The magnification is 10×, dash lines represent epidermis. Panel c shows the collagen abundance measured from the staining intensity (*p <0.05).

Mentions: In order to explore the effect of estrogen on collagen content in ovine dermal skin, we performed Sirius red staining followed by polarized light microscopic imaging. Because the birefringence is highly specific to fibrillar collagen due to its uniaxial anisotropy,(Cuttle et al., 2005; Junqueira et al., 1978) the staining serves as a good indication of collagen fibril abundance. Figure 4 indicates higher abundance of fibrillar collagen in Sham dermis (p<0.05) and a qualitatively thicker fibril bundle width than in OVX dermis.


Estrogen depletion results in nanoscale morphology changes in dermal collagen.

Fang M, Liroff KG, Turner AS, Les CM, Orr BG, Holl MM - J. Invest. Dermatol. (2012)

Sirius red staining reveals the abundance of fibrillar collagen content in Sham and OVX dermis. Panel a and b are polarized light microscopic images of Sham and OVX dermis, respectively. The magnification is 10×, dash lines represent epidermis. Panel c shows the collagen abundance measured from the staining intensity (*p <0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375339&req=5

Figure 4: Sirius red staining reveals the abundance of fibrillar collagen content in Sham and OVX dermis. Panel a and b are polarized light microscopic images of Sham and OVX dermis, respectively. The magnification is 10×, dash lines represent epidermis. Panel c shows the collagen abundance measured from the staining intensity (*p <0.05).
Mentions: In order to explore the effect of estrogen on collagen content in ovine dermal skin, we performed Sirius red staining followed by polarized light microscopic imaging. Because the birefringence is highly specific to fibrillar collagen due to its uniaxial anisotropy,(Cuttle et al., 2005; Junqueira et al., 1978) the staining serves as a good indication of collagen fibril abundance. Figure 4 indicates higher abundance of fibrillar collagen in Sham dermis (p<0.05) and a qualitatively thicker fibril bundle width than in OVX dermis.

Bottom Line: In addition, the overall width of the distribution, both values above and below the mean, was found to be increased.The change in width due to an increase in lower values of D-spacings was previously reported for ovine bone; however, this report demonstrates that the effect is also present in non-mineralized collagen fibrils.A nonparametric Kolmogorov-Smirnov test of the cumulative density function indicates a statistical difference in the sham and OVX D-spacing distributions (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.

ABSTRACT
Tissue cryo-sectioning combined with atomic force microscopy imaging reveals that the nanoscale morphology of dermal collagen fibrils, quantified using the metric of D-periodic spacing, changes under the condition of estrogen depletion. Specifically, a new subpopulation of fibrils with D-spacings in the region between 56 and 59 nm is present 2 years following ovariectomy in ovine dermal samples. In addition, the overall width of the distribution, both values above and below the mean, was found to be increased. The change in width due to an increase in lower values of D-spacings was previously reported for ovine bone; however, this report demonstrates that the effect is also present in non-mineralized collagen fibrils. A nonparametric Kolmogorov-Smirnov test of the cumulative density function indicates a statistical difference in the sham and OVX D-spacing distributions (P<0.01).

Show MeSH
Related in: MedlinePlus