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Distribution of HLA-A, -B and -DRB1 genes and haplotypes in the Tujia population living in the Wufeng Region of Hubei Province, China.

Zhang L, Cheng D, Tao N, Zhao M, Zhang F, Yuan Y, Qiu X - PLoS ONE (2012)

Bottom Line: The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%).Fourteen two-loci haplotypes had significant linkage disequilibrium.These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, Medical School of Wuhan University, Wuhan, Hubei, China.

ABSTRACT

Background: The distribution of HLA alleles and haplotypes varies widely between different ethnic populations and geographic areas. Before any genetic marker can be used in a disease-associated study it is therefore essential to investigate allelic frequencies and establish a genetic database.

Methodology/principal findings: This is the first report of HLA typing in the Tujia group using the Luminex HLA-SSO method HLA-A, -B and -DRB1 allelic distributions were determined in 124 unrelated healthy Tujia individuals, and haplotypic frequencies and linkage disequilibrium parameters were estimated using the maximum-likelihood method. In total 10 alleles were detected at the HLA-A locus, 21 alleles at the HLA-B locus and 14 alleles at the HLA-DRB1 locus. The most frequently observed alleles in the HLA-I group were HLA-A*02 (35.48%), A*11 (28.23%), A*24 (15.73%); HLA-B*40 (25.00%), B*46 (16.13%), and B*15 (15.73%). Among HLA-DRB1 alleles, high frequencies of HLA-DRB1*09 (25.81%) were observed, followed by HLA-DRB1*15 (12.9%), and DRB1*12 (10.89%). The two-locus haplotypes at the highest frequency were A*02-B*46A (8.47%), followed by A*11-B*40 (7.66%), A*02-B*40 (8.87%), A*11-B*15 (6.45%), A*02-B*15 (6.05%), B*40-DRB1*09 (9.27%) and B*46-DRB1*09 (6.45%). The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%). Fourteen two-loci haplotypes had significant linkage disequilibrium. Construction of a neighbor-joining phylogenetic tree and principal component analysis using the allelic frequencies at HLA-A was performed to compare the Tujia group and twelve other previously reported populations. The Tujia population in the Wufeng of Hubei Province had the closest genetic relationship with the central Han population, and then to the Shui, the Miao, the southern Han and the northern Han ethnic groups.

Conclusions/significance: These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

Show MeSH
Phylogeny based on HLA allele frequencies.Dendroram constructed by the neighbor-joining method showing the relationship between Tujia populations in Hubei with other populations in Chinese individuals of 12 regions based on the frequencies of HLA-A loci.
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pone-0038774-g002: Phylogeny based on HLA allele frequencies.Dendroram constructed by the neighbor-joining method showing the relationship between Tujia populations in Hubei with other populations in Chinese individuals of 12 regions based on the frequencies of HLA-A loci.

Mentions: The phylogenetic tree shown in Figure 2 was constructed using the allelic frequencies at the HLA-A locus of the Tujia population in Wufeng and other ethnic groups. We compared the Tujia population (number of individuals analyzed:124) to the Uyghur (n = 104) [5], southern Han (n = 172), northern Han (n = 152), central Han (n = 211), Min-nan (n = 7137), Taiwan aborigines (n = 111), Miao (n = 154) [6], Hui (n = 122) [7], Shui (n = 153), Bouyei (n = 109) [8], Bulang (n = 116) and Hani (n = 150) [9].The phylogenetic tree is shown in Fig. 2. The genetic structure of the Tujia is closest to the central Han population.


Distribution of HLA-A, -B and -DRB1 genes and haplotypes in the Tujia population living in the Wufeng Region of Hubei Province, China.

Zhang L, Cheng D, Tao N, Zhao M, Zhang F, Yuan Y, Qiu X - PLoS ONE (2012)

Phylogeny based on HLA allele frequencies.Dendroram constructed by the neighbor-joining method showing the relationship between Tujia populations in Hubei with other populations in Chinese individuals of 12 regions based on the frequencies of HLA-A loci.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375274&req=5

pone-0038774-g002: Phylogeny based on HLA allele frequencies.Dendroram constructed by the neighbor-joining method showing the relationship between Tujia populations in Hubei with other populations in Chinese individuals of 12 regions based on the frequencies of HLA-A loci.
Mentions: The phylogenetic tree shown in Figure 2 was constructed using the allelic frequencies at the HLA-A locus of the Tujia population in Wufeng and other ethnic groups. We compared the Tujia population (number of individuals analyzed:124) to the Uyghur (n = 104) [5], southern Han (n = 172), northern Han (n = 152), central Han (n = 211), Min-nan (n = 7137), Taiwan aborigines (n = 111), Miao (n = 154) [6], Hui (n = 122) [7], Shui (n = 153), Bouyei (n = 109) [8], Bulang (n = 116) and Hani (n = 150) [9].The phylogenetic tree is shown in Fig. 2. The genetic structure of the Tujia is closest to the central Han population.

Bottom Line: The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%).Fourteen two-loci haplotypes had significant linkage disequilibrium.These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, Medical School of Wuhan University, Wuhan, Hubei, China.

ABSTRACT

Background: The distribution of HLA alleles and haplotypes varies widely between different ethnic populations and geographic areas. Before any genetic marker can be used in a disease-associated study it is therefore essential to investigate allelic frequencies and establish a genetic database.

Methodology/principal findings: This is the first report of HLA typing in the Tujia group using the Luminex HLA-SSO method HLA-A, -B and -DRB1 allelic distributions were determined in 124 unrelated healthy Tujia individuals, and haplotypic frequencies and linkage disequilibrium parameters were estimated using the maximum-likelihood method. In total 10 alleles were detected at the HLA-A locus, 21 alleles at the HLA-B locus and 14 alleles at the HLA-DRB1 locus. The most frequently observed alleles in the HLA-I group were HLA-A*02 (35.48%), A*11 (28.23%), A*24 (15.73%); HLA-B*40 (25.00%), B*46 (16.13%), and B*15 (15.73%). Among HLA-DRB1 alleles, high frequencies of HLA-DRB1*09 (25.81%) were observed, followed by HLA-DRB1*15 (12.9%), and DRB1*12 (10.89%). The two-locus haplotypes at the highest frequency were A*02-B*46A (8.47%), followed by A*11-B*40 (7.66%), A*02-B*40 (8.87%), A*11-B*15 (6.45%), A*02-B*15 (6.05%), B*40-DRB1*09 (9.27%) and B*46-DRB1*09 (6.45%). The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%). Fourteen two-loci haplotypes had significant linkage disequilibrium. Construction of a neighbor-joining phylogenetic tree and principal component analysis using the allelic frequencies at HLA-A was performed to compare the Tujia group and twelve other previously reported populations. The Tujia population in the Wufeng of Hubei Province had the closest genetic relationship with the central Han population, and then to the Shui, the Miao, the southern Han and the northern Han ethnic groups.

Conclusions/significance: These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

Show MeSH