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Distribution of HLA-A, -B and -DRB1 genes and haplotypes in the Tujia population living in the Wufeng Region of Hubei Province, China.

Zhang L, Cheng D, Tao N, Zhao M, Zhang F, Yuan Y, Qiu X - PLoS ONE (2012)

Bottom Line: The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%).Fourteen two-loci haplotypes had significant linkage disequilibrium.These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, Medical School of Wuhan University, Wuhan, Hubei, China.

ABSTRACT

Background: The distribution of HLA alleles and haplotypes varies widely between different ethnic populations and geographic areas. Before any genetic marker can be used in a disease-associated study it is therefore essential to investigate allelic frequencies and establish a genetic database.

Methodology/principal findings: This is the first report of HLA typing in the Tujia group using the Luminex HLA-SSO method HLA-A, -B and -DRB1 allelic distributions were determined in 124 unrelated healthy Tujia individuals, and haplotypic frequencies and linkage disequilibrium parameters were estimated using the maximum-likelihood method. In total 10 alleles were detected at the HLA-A locus, 21 alleles at the HLA-B locus and 14 alleles at the HLA-DRB1 locus. The most frequently observed alleles in the HLA-I group were HLA-A*02 (35.48%), A*11 (28.23%), A*24 (15.73%); HLA-B*40 (25.00%), B*46 (16.13%), and B*15 (15.73%). Among HLA-DRB1 alleles, high frequencies of HLA-DRB1*09 (25.81%) were observed, followed by HLA-DRB1*15 (12.9%), and DRB1*12 (10.89%). The two-locus haplotypes at the highest frequency were A*02-B*46A (8.47%), followed by A*11-B*40 (7.66%), A*02-B*40 (8.87%), A*11-B*15 (6.45%), A*02-B*15 (6.05%), B*40-DRB1*09 (9.27%) and B*46-DRB1*09 (6.45%). The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%). Fourteen two-loci haplotypes had significant linkage disequilibrium. Construction of a neighbor-joining phylogenetic tree and principal component analysis using the allelic frequencies at HLA-A was performed to compare the Tujia group and twelve other previously reported populations. The Tujia population in the Wufeng of Hubei Province had the closest genetic relationship with the central Han population, and then to the Shui, the Miao, the southern Han and the northern Han ethnic groups.

Conclusions/significance: These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

Show MeSH
Map showing the sites of the populations used in this study.1,Uyghur 2,Southern-Han 3,Tujia 4,Miao 5,Hui 6,Northern-Han 7,Bouyei 8,Shui 9,Bulang 10,Hani 11,Minnan 12,Taiwan-Aborigines 13,Middle-Han.
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pone-0038774-g001: Map showing the sites of the populations used in this study.1,Uyghur 2,Southern-Han 3,Tujia 4,Miao 5,Hui 6,Northern-Han 7,Bouyei 8,Shui 9,Bulang 10,Hani 11,Minnan 12,Taiwan-Aborigines 13,Middle-Han.

Mentions: In this study, one hundred twenty-four non-blood relationship Tujia from Wufeng, Hubei province were recruited and their HLA alleles classified into HLA–A, –B or –DRB1 loci alleles using a WAKFlow HLA typing kit (Luminex HLA-SSO; Luminex HLA-SSO Inc., Shanghai, China) on the Multi-Analyte Profiling system (xMAP). The allele and haplotype frequency of HLA loci were calculated and their allele frequencies were compared with those of other ethnic groups (Fig. 1). The aims of this study were: 1) To outline the heritage status of HLA–A, –B and DRB1 loci in the Tujia inhabiting the Wufeng region. 2) To study the cause of genetic heterogeneity among Wufeng Tujia and the stage of the Wufeng Tujia among other ethnic groups in the whole process of human evolution. This study will help us to further understand the genetic background of HLA–A, –B and DRB1 loci alleles and their relationship to disease in the Wufeng Tujia population.


Distribution of HLA-A, -B and -DRB1 genes and haplotypes in the Tujia population living in the Wufeng Region of Hubei Province, China.

Zhang L, Cheng D, Tao N, Zhao M, Zhang F, Yuan Y, Qiu X - PLoS ONE (2012)

Map showing the sites of the populations used in this study.1,Uyghur 2,Southern-Han 3,Tujia 4,Miao 5,Hui 6,Northern-Han 7,Bouyei 8,Shui 9,Bulang 10,Hani 11,Minnan 12,Taiwan-Aborigines 13,Middle-Han.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375274&req=5

pone-0038774-g001: Map showing the sites of the populations used in this study.1,Uyghur 2,Southern-Han 3,Tujia 4,Miao 5,Hui 6,Northern-Han 7,Bouyei 8,Shui 9,Bulang 10,Hani 11,Minnan 12,Taiwan-Aborigines 13,Middle-Han.
Mentions: In this study, one hundred twenty-four non-blood relationship Tujia from Wufeng, Hubei province were recruited and their HLA alleles classified into HLA–A, –B or –DRB1 loci alleles using a WAKFlow HLA typing kit (Luminex HLA-SSO; Luminex HLA-SSO Inc., Shanghai, China) on the Multi-Analyte Profiling system (xMAP). The allele and haplotype frequency of HLA loci were calculated and their allele frequencies were compared with those of other ethnic groups (Fig. 1). The aims of this study were: 1) To outline the heritage status of HLA–A, –B and DRB1 loci in the Tujia inhabiting the Wufeng region. 2) To study the cause of genetic heterogeneity among Wufeng Tujia and the stage of the Wufeng Tujia among other ethnic groups in the whole process of human evolution. This study will help us to further understand the genetic background of HLA–A, –B and DRB1 loci alleles and their relationship to disease in the Wufeng Tujia population.

Bottom Line: The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%).Fourteen two-loci haplotypes had significant linkage disequilibrium.These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

View Article: PubMed Central - PubMed

Affiliation: Institute of Virology, Medical School of Wuhan University, Wuhan, Hubei, China.

ABSTRACT

Background: The distribution of HLA alleles and haplotypes varies widely between different ethnic populations and geographic areas. Before any genetic marker can be used in a disease-associated study it is therefore essential to investigate allelic frequencies and establish a genetic database.

Methodology/principal findings: This is the first report of HLA typing in the Tujia group using the Luminex HLA-SSO method HLA-A, -B and -DRB1 allelic distributions were determined in 124 unrelated healthy Tujia individuals, and haplotypic frequencies and linkage disequilibrium parameters were estimated using the maximum-likelihood method. In total 10 alleles were detected at the HLA-A locus, 21 alleles at the HLA-B locus and 14 alleles at the HLA-DRB1 locus. The most frequently observed alleles in the HLA-I group were HLA-A*02 (35.48%), A*11 (28.23%), A*24 (15.73%); HLA-B*40 (25.00%), B*46 (16.13%), and B*15 (15.73%). Among HLA-DRB1 alleles, high frequencies of HLA-DRB1*09 (25.81%) were observed, followed by HLA-DRB1*15 (12.9%), and DRB1*12 (10.89%). The two-locus haplotypes at the highest frequency were A*02-B*46A (8.47%), followed by A*11-B*40 (7.66%), A*02-B*40 (8.87%), A*11-B*15 (6.45%), A*02-B*15 (6.05%), B*40-DRB1*09 (9.27%) and B*46-DRB1*09 (6.45%). The most common three-locus haplotypes found in the Tujia population were A*02-B*46-DRB1*09 (4.84%) and A*02-B*40-DRB1*09 (4.03%). Fourteen two-loci haplotypes had significant linkage disequilibrium. Construction of a neighbor-joining phylogenetic tree and principal component analysis using the allelic frequencies at HLA-A was performed to compare the Tujia group and twelve other previously reported populations. The Tujia population in the Wufeng of Hubei Province had the closest genetic relationship with the central Han population, and then to the Shui, the Miao, the southern Han and the northern Han ethnic groups.

Conclusions/significance: These results will become a valuable source of data for tracing population migration, planning clinical organ transplantation, carrying out HLA-linked disease-associated studies and forensic identification.

Show MeSH