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Histological evaluation of corneal scar formation in pseudophakic bullous keratopathy.

Liu T, Xu Y, Sun D, Xie L - PLoS ONE (2012)

Bottom Line: The histological evaluation and scoring of the buttons for morphological changes, including the degree of stromal scars, neovascularization and inflammatory cells within the corneal buttons, were compared.Confocal microscopy examination and histological evaluation and scoring of the buttons showed that compared with the <1.0 year group, stromal scars, neovascularization and inflammatory cells were more severe in the >1.0 year group (P<0.05).Cytokines such as CTGF and TGF-β1 may play a role in this pathological process and deserve further investigation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.

ABSTRACT

Purpose: To evaluate histological changes in the corneal stroma in pseudophakic bullous keratopathy.

Methods: Twenty-eight patients (28 eyes) with pseudophakic bullous keratopathy underwent therapeutic penetrating keratoplasty at Shandong Eye Institute between January 2006 and November 2011. The patients were divided into two groups according to the duration of bullous keratopathy (<1.0 year group or >1.0 year group), and three buttons from enucleated eyes with choroidal melanoma served as a control. In vivo confocal microscopy examination, hematoxylin-eosin, Masson's trichrome stain and Van Gieson staining were used for microscopic examination. The histological evaluation and scoring of the buttons for morphological changes, including the degree of stromal scars, neovascularization and inflammatory cells within the corneal buttons, were compared. To study the underlying mechanism, connective tissue growth factor (CTGF) and TGF-β immunohistochemistry were performed.

Results: Confocal microscopy examination and histological evaluation and scoring of the buttons showed that compared with the <1.0 year group, stromal scars, neovascularization and inflammatory cells were more severe in the >1.0 year group (P<0.05). There was an increase in CTGF- and TGF-β1-positive stromal cells in the >1.0 year group.

Conclusions: During the progression of pseudophakic bullous keratopathy, stromal scars occurred more often in the patients that had a longer duration of disease. Cytokines such as CTGF and TGF-β1 may play a role in this pathological process and deserve further investigation.

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A patient with a 3-year history of pseudophakic bullous keratopathy.(A) A slit-lamp photograph of the cornea showing dense scarring and neovascularization; (B) in the histological sections, obvious intense scarring, neovascularization and inflammatory cells can be observed; (C) Masson's trichrome staining; and (D) Van Gieson staining. Scale bar 50 µm.
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pone-0039201-g004: A patient with a 3-year history of pseudophakic bullous keratopathy.(A) A slit-lamp photograph of the cornea showing dense scarring and neovascularization; (B) in the histological sections, obvious intense scarring, neovascularization and inflammatory cells can be observed; (C) Masson's trichrome staining; and (D) Van Gieson staining. Scale bar 50 µm.

Mentions: In the <1.0 year group, corneal stromal fiber crosslinking was found in the deep stroma, and no dense deep corneal stromal scars were found. In addition, neither neovascularization nor inflammatory cell infiltration was found (Figure 2). In the >1.0 year group, the epithelial and stromal pathological changes were more extended and severe. There were dense corneal scars, neovascularization and inflammatory cell infiltration in the stroma. Anterior corneal scars and broken Bowman’s membranes were also observed (Figures 3, 4).


Histological evaluation of corneal scar formation in pseudophakic bullous keratopathy.

Liu T, Xu Y, Sun D, Xie L - PLoS ONE (2012)

A patient with a 3-year history of pseudophakic bullous keratopathy.(A) A slit-lamp photograph of the cornea showing dense scarring and neovascularization; (B) in the histological sections, obvious intense scarring, neovascularization and inflammatory cells can be observed; (C) Masson's trichrome staining; and (D) Van Gieson staining. Scale bar 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375240&req=5

pone-0039201-g004: A patient with a 3-year history of pseudophakic bullous keratopathy.(A) A slit-lamp photograph of the cornea showing dense scarring and neovascularization; (B) in the histological sections, obvious intense scarring, neovascularization and inflammatory cells can be observed; (C) Masson's trichrome staining; and (D) Van Gieson staining. Scale bar 50 µm.
Mentions: In the <1.0 year group, corneal stromal fiber crosslinking was found in the deep stroma, and no dense deep corneal stromal scars were found. In addition, neither neovascularization nor inflammatory cell infiltration was found (Figure 2). In the >1.0 year group, the epithelial and stromal pathological changes were more extended and severe. There were dense corneal scars, neovascularization and inflammatory cell infiltration in the stroma. Anterior corneal scars and broken Bowman’s membranes were also observed (Figures 3, 4).

Bottom Line: The histological evaluation and scoring of the buttons for morphological changes, including the degree of stromal scars, neovascularization and inflammatory cells within the corneal buttons, were compared.Confocal microscopy examination and histological evaluation and scoring of the buttons showed that compared with the <1.0 year group, stromal scars, neovascularization and inflammatory cells were more severe in the >1.0 year group (P<0.05).Cytokines such as CTGF and TGF-β1 may play a role in this pathological process and deserve further investigation.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.

ABSTRACT

Purpose: To evaluate histological changes in the corneal stroma in pseudophakic bullous keratopathy.

Methods: Twenty-eight patients (28 eyes) with pseudophakic bullous keratopathy underwent therapeutic penetrating keratoplasty at Shandong Eye Institute between January 2006 and November 2011. The patients were divided into two groups according to the duration of bullous keratopathy (<1.0 year group or >1.0 year group), and three buttons from enucleated eyes with choroidal melanoma served as a control. In vivo confocal microscopy examination, hematoxylin-eosin, Masson's trichrome stain and Van Gieson staining were used for microscopic examination. The histological evaluation and scoring of the buttons for morphological changes, including the degree of stromal scars, neovascularization and inflammatory cells within the corneal buttons, were compared. To study the underlying mechanism, connective tissue growth factor (CTGF) and TGF-β immunohistochemistry were performed.

Results: Confocal microscopy examination and histological evaluation and scoring of the buttons showed that compared with the <1.0 year group, stromal scars, neovascularization and inflammatory cells were more severe in the >1.0 year group (P<0.05). There was an increase in CTGF- and TGF-β1-positive stromal cells in the >1.0 year group.

Conclusions: During the progression of pseudophakic bullous keratopathy, stromal scars occurred more often in the patients that had a longer duration of disease. Cytokines such as CTGF and TGF-β1 may play a role in this pathological process and deserve further investigation.

Show MeSH
Related in: MedlinePlus