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Emergence of dengue virus 4 genotype II in Guangzhou, China, 2010: survey and molecular epidemiology of one community outbreak.

Jing QL, Yang ZC, Luo L, Xiao XC, Di B, He P, Fu CX, Wang M, Lu JH - BMC Infect. Dis. (2012)

Bottom Line: However, it has not been known to have caused a local outbreak in China for the past 20 years.During September and October of 2010, one imported case, a Guangzhou resident who travelled back from Thailand, resulted in 18 secondary autochthonous cases in Guangzhou City, with an incidence rate of 5.53 per 10,000 residents.In indigenous cases, 14 serum samples tested positive for IgM against DENV and 7 for IgG from a total of 15 submitted serum samples, accompanied by 5 DENV-4 isolates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou, People's Republic of China. gdgzcdc@163.com

ABSTRACT

Background: The re-emergence of dengue virus 4 (DENV-4) has become a public health concern in South America, Southeast Asia and South Asia. However, it has not been known to have caused a local outbreak in China for the past 20 years. The purpose of this study was to elucidate the epidemiology of one local community outbreak caused by DENV-4 in Guangzhou city, China, in 2010; and to determine the molecular characteristics of the genotype II virus involved.

Case presentations: During September and October of 2010, one imported case, a Guangzhou resident who travelled back from Thailand, resulted in 18 secondary autochthonous cases in Guangzhou City, with an incidence rate of 5.53 per 10,000 residents. In indigenous cases, 14 serum samples tested positive for IgM against DENV and 7 for IgG from a total of 15 submitted serum samples, accompanied by 5 DENV-4 isolates. With identical envelope gene nucleotide sequences, the two isolates (D10168-GZ from the imported index case and Guangzhou 10660 from the first isolate in the autochthonous cases) were grouped into DENV-4 genotype II after comparison to 32 previous DENV-4 isolates from GenBank that originated from different areas.

Conclusions: Based on epidemiological and phylogenetic analyses, the outbreak, which was absent for 20 years after the DENV-4 genotype I outbreak in 1990, was confirmed as DENV-4 genotype II and initially traced to the imported index case, a Guangzhou resident who travelled back from Thailand.

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The phylogenetic analysis of the envelope gene sequences of DENV-4. The phylogenetic tree was constructed by the neighbour-joining method with a Kimura 2 parameter model using MEGA 4.0 software. Bootstrap values were set for 1000 repetitions and were placed over each main node of the tree. Name rule: country/strain name/isolated year/GenBank accession number. CL: Chile; CN: China; DM: Dominica; ID: Indonesia; IN: India; KH: Cambodia; MM: Myanmar; MY: Malaysia; PE: Peru; PH: the Philippines; PR: Puerto Rico; SB: Solomon; SG: Singapore; TH: Thailand; VE: Venezuela.
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Figure 2: The phylogenetic analysis of the envelope gene sequences of DENV-4. The phylogenetic tree was constructed by the neighbour-joining method with a Kimura 2 parameter model using MEGA 4.0 software. Bootstrap values were set for 1000 repetitions and were placed over each main node of the tree. Name rule: country/strain name/isolated year/GenBank accession number. CL: Chile; CN: China; DM: Dominica; ID: Indonesia; IN: India; KH: Cambodia; MM: Myanmar; MY: Malaysia; PE: Peru; PH: the Philippines; PR: Puerto Rico; SB: Solomon; SG: Singapore; TH: Thailand; VE: Venezuela.

Mentions: Phylogenetically, as demonstrated in Figure 2, Guangzhou 10660 and D10168-GZ are identical, and are located in the same clade and grouped into DENV-4 genotype II. These strains are closely related to 02-12-1HuNIID, and are clustered within the same minor clade as 0712TW isolated in Taiwan in 2007 and imported from Indonesia, SW36i isolated in Indonesia in 2004, and 2641Y08 isolated in Singapore in 2008. These strains are distantly related to strain CN78-56 which was grouped into the genotype II clade which was isolated in China in 1978, and remarkably remote from the Guangzhou B5 strain which isolated in China in 1990 and was grouped to the genotype I clade.


Emergence of dengue virus 4 genotype II in Guangzhou, China, 2010: survey and molecular epidemiology of one community outbreak.

Jing QL, Yang ZC, Luo L, Xiao XC, Di B, He P, Fu CX, Wang M, Lu JH - BMC Infect. Dis. (2012)

The phylogenetic analysis of the envelope gene sequences of DENV-4. The phylogenetic tree was constructed by the neighbour-joining method with a Kimura 2 parameter model using MEGA 4.0 software. Bootstrap values were set for 1000 repetitions and were placed over each main node of the tree. Name rule: country/strain name/isolated year/GenBank accession number. CL: Chile; CN: China; DM: Dominica; ID: Indonesia; IN: India; KH: Cambodia; MM: Myanmar; MY: Malaysia; PE: Peru; PH: the Philippines; PR: Puerto Rico; SB: Solomon; SG: Singapore; TH: Thailand; VE: Venezuela.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375192&req=5

Figure 2: The phylogenetic analysis of the envelope gene sequences of DENV-4. The phylogenetic tree was constructed by the neighbour-joining method with a Kimura 2 parameter model using MEGA 4.0 software. Bootstrap values were set for 1000 repetitions and were placed over each main node of the tree. Name rule: country/strain name/isolated year/GenBank accession number. CL: Chile; CN: China; DM: Dominica; ID: Indonesia; IN: India; KH: Cambodia; MM: Myanmar; MY: Malaysia; PE: Peru; PH: the Philippines; PR: Puerto Rico; SB: Solomon; SG: Singapore; TH: Thailand; VE: Venezuela.
Mentions: Phylogenetically, as demonstrated in Figure 2, Guangzhou 10660 and D10168-GZ are identical, and are located in the same clade and grouped into DENV-4 genotype II. These strains are closely related to 02-12-1HuNIID, and are clustered within the same minor clade as 0712TW isolated in Taiwan in 2007 and imported from Indonesia, SW36i isolated in Indonesia in 2004, and 2641Y08 isolated in Singapore in 2008. These strains are distantly related to strain CN78-56 which was grouped into the genotype II clade which was isolated in China in 1978, and remarkably remote from the Guangzhou B5 strain which isolated in China in 1990 and was grouped to the genotype I clade.

Bottom Line: However, it has not been known to have caused a local outbreak in China for the past 20 years.During September and October of 2010, one imported case, a Guangzhou resident who travelled back from Thailand, resulted in 18 secondary autochthonous cases in Guangzhou City, with an incidence rate of 5.53 per 10,000 residents.In indigenous cases, 14 serum samples tested positive for IgM against DENV and 7 for IgG from a total of 15 submitted serum samples, accompanied by 5 DENV-4 isolates.

View Article: PubMed Central - HTML - PubMed

Affiliation: Guangzhou Center for Disease Control and Prevention, Guangzhou, People's Republic of China. gdgzcdc@163.com

ABSTRACT

Background: The re-emergence of dengue virus 4 (DENV-4) has become a public health concern in South America, Southeast Asia and South Asia. However, it has not been known to have caused a local outbreak in China for the past 20 years. The purpose of this study was to elucidate the epidemiology of one local community outbreak caused by DENV-4 in Guangzhou city, China, in 2010; and to determine the molecular characteristics of the genotype II virus involved.

Case presentations: During September and October of 2010, one imported case, a Guangzhou resident who travelled back from Thailand, resulted in 18 secondary autochthonous cases in Guangzhou City, with an incidence rate of 5.53 per 10,000 residents. In indigenous cases, 14 serum samples tested positive for IgM against DENV and 7 for IgG from a total of 15 submitted serum samples, accompanied by 5 DENV-4 isolates. With identical envelope gene nucleotide sequences, the two isolates (D10168-GZ from the imported index case and Guangzhou 10660 from the first isolate in the autochthonous cases) were grouped into DENV-4 genotype II after comparison to 32 previous DENV-4 isolates from GenBank that originated from different areas.

Conclusions: Based on epidemiological and phylogenetic analyses, the outbreak, which was absent for 20 years after the DENV-4 genotype I outbreak in 1990, was confirmed as DENV-4 genotype II and initially traced to the imported index case, a Guangzhou resident who travelled back from Thailand.

Show MeSH
Related in: MedlinePlus