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Deoiledjatropha seed cake is a useful nutrient for pullulan production.

Choudhury AR, Sharma N, Prasad GS - Microb. Cell Fact. (2012)

Bottom Line: In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially.The process was further validated in a 5 L laboratory scale fermenter.This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemical Engineering Research & Process Development Centre, CSIR-Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh, India. anirban@imtech.res.in

ABSTRACT

Background: Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially. De-oiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of bio-diesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem.Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of bio-diesel industry.

Results: In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter.

Conclusion: This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.

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Mentions: Incubation temperature is strain dependent and has significant influence on growth and production of pullulan during fermentation [19] suggesting the need to determine optimum temperature to maximize pullulan production. The incubation temperature was varied from 15°C to 30°C to examine the effect of the same on pullulan production. Optimum pullulan production was obtained at 28°C (82.91 g/L) and it was almost twice as compared to the production at 15°C (Figure 2A). However, as the temperature was increased to 30°C there was significant reduction in pullulan production (45.11 g/L). This observation is in agreement with the observations of Roukas and Biliaderis [20], but different from other report which shows optimal pullulan production at 25°C [21]. This confirms the earlier observations that the temperature optimum for production of pullulan is strain specific and also often corresponds to the optimum temperature of growth of the microorganism used for production.


Deoiledjatropha seed cake is a useful nutrient for pullulan production.

Choudhury AR, Sharma N, Prasad GS - Microb. Cell Fact. (2012)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3375191&req=5

Mentions: Incubation temperature is strain dependent and has significant influence on growth and production of pullulan during fermentation [19] suggesting the need to determine optimum temperature to maximize pullulan production. The incubation temperature was varied from 15°C to 30°C to examine the effect of the same on pullulan production. Optimum pullulan production was obtained at 28°C (82.91 g/L) and it was almost twice as compared to the production at 15°C (Figure 2A). However, as the temperature was increased to 30°C there was significant reduction in pullulan production (45.11 g/L). This observation is in agreement with the observations of Roukas and Biliaderis [20], but different from other report which shows optimal pullulan production at 25°C [21]. This confirms the earlier observations that the temperature optimum for production of pullulan is strain specific and also often corresponds to the optimum temperature of growth of the microorganism used for production.

Bottom Line: In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially.The process was further validated in a 5 L laboratory scale fermenter.This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.

View Article: PubMed Central - HTML - PubMed

Affiliation: Biochemical Engineering Research & Process Development Centre, CSIR-Institute of Microbial Technology, Council of Scientific and Industrial Research, Chandigarh, India. anirban@imtech.res.in

ABSTRACT

Background: Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially. De-oiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of bio-diesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem.Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of bio-diesel industry.

Results: In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter.

Conclusion: This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.

Show MeSH
Related in: MedlinePlus