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A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain.

Sunwoo YY, Park SI, Chung YA, Lee J, Park MS, Jang KS, Maeng LS, Jang DK, Im R, Jung YJ, Park SA, Kang ES, Kim MW, Han YM - Evid Based Complement Alternat Med (2012)

Bottom Line: Behavior tests demonstrated a significant improvement compared to the control.Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area.These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.

ABSTRACT
In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

No MeSH data available.


Related in: MedlinePlus

Expression of growth factors and inflammatory cytokines in the ischemic rat brain. BDNF, VEGF, Cox-2, TNF-α, IL-1β, and IL-1α were detected by ELISA at 14 days after MCAo. Protein levels of these factors show the quantitative analysis data (a–f). The expression of BDNF and VEGF was significantly increased compared with the control group at 14 days after MCAo (a, b). Also, inflammatory cytokines such as Cox-2, TNF-α, IL-1β, and IL-1α were significantly decreased compared with the control group (c, d, e, f). Data are expressed as mean ± SD, *P < 0.05.
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fig5: Expression of growth factors and inflammatory cytokines in the ischemic rat brain. BDNF, VEGF, Cox-2, TNF-α, IL-1β, and IL-1α were detected by ELISA at 14 days after MCAo. Protein levels of these factors show the quantitative analysis data (a–f). The expression of BDNF and VEGF was significantly increased compared with the control group at 14 days after MCAo (a, b). Also, inflammatory cytokines such as Cox-2, TNF-α, IL-1β, and IL-1α were significantly decreased compared with the control group (c, d, e, f). Data are expressed as mean ± SD, *P < 0.05.

Mentions: We hypothesized that gongjin-dan may protect an injured area following a stroke by elevating neurotrophic factors and by reducing inflammatory cytokines. We used the ELISA to detect protein levels of neurotrophic factors and inflammatory cytokines in the ischemic rat brain. At 14 days after MCAo, expression of BDNF and VEGF was significantly high compared to the control group (Figures 5(a) and 5(b)). Also inflammatory cytokines such as Cox-2, IL-1α, IL-1β, and TNF-α were reduced as compared with control group (Figures 5(c), 5(d), 5(e), and 5(f)). These data suggest that an increment of neurotrophic factors and a decrease of inflammatory cytokines by gongjin-dan could protect cerebral infarction and promote behavior function recovery.


A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain.

Sunwoo YY, Park SI, Chung YA, Lee J, Park MS, Jang KS, Maeng LS, Jang DK, Im R, Jung YJ, Park SA, Kang ES, Kim MW, Han YM - Evid Based Complement Alternat Med (2012)

Expression of growth factors and inflammatory cytokines in the ischemic rat brain. BDNF, VEGF, Cox-2, TNF-α, IL-1β, and IL-1α were detected by ELISA at 14 days after MCAo. Protein levels of these factors show the quantitative analysis data (a–f). The expression of BDNF and VEGF was significantly increased compared with the control group at 14 days after MCAo (a, b). Also, inflammatory cytokines such as Cox-2, TNF-α, IL-1β, and IL-1α were significantly decreased compared with the control group (c, d, e, f). Data are expressed as mean ± SD, *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375177&req=5

fig5: Expression of growth factors and inflammatory cytokines in the ischemic rat brain. BDNF, VEGF, Cox-2, TNF-α, IL-1β, and IL-1α were detected by ELISA at 14 days after MCAo. Protein levels of these factors show the quantitative analysis data (a–f). The expression of BDNF and VEGF was significantly increased compared with the control group at 14 days after MCAo (a, b). Also, inflammatory cytokines such as Cox-2, TNF-α, IL-1β, and IL-1α were significantly decreased compared with the control group (c, d, e, f). Data are expressed as mean ± SD, *P < 0.05.
Mentions: We hypothesized that gongjin-dan may protect an injured area following a stroke by elevating neurotrophic factors and by reducing inflammatory cytokines. We used the ELISA to detect protein levels of neurotrophic factors and inflammatory cytokines in the ischemic rat brain. At 14 days after MCAo, expression of BDNF and VEGF was significantly high compared to the control group (Figures 5(a) and 5(b)). Also inflammatory cytokines such as Cox-2, IL-1α, IL-1β, and TNF-α were reduced as compared with control group (Figures 5(c), 5(d), 5(e), and 5(f)). These data suggest that an increment of neurotrophic factors and a decrease of inflammatory cytokines by gongjin-dan could protect cerebral infarction and promote behavior function recovery.

Bottom Line: Behavior tests demonstrated a significant improvement compared to the control.Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area.These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.

ABSTRACT
In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

No MeSH data available.


Related in: MedlinePlus