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A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain.

Sunwoo YY, Park SI, Chung YA, Lee J, Park MS, Jang KS, Maeng LS, Jang DK, Im R, Jung YJ, Park SA, Kang ES, Kim MW, Han YM - Evid Based Complement Alternat Med (2012)

Bottom Line: Behavior tests demonstrated a significant improvement compared to the control.Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area.These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.

ABSTRACT
In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

No MeSH data available.


Related in: MedlinePlus

Effect of gongjin-dan on infarct volume, glucose metabolism, and regional cerebral blood flow (rCBF) after MCAo. Images shown in the PET images and TTC-stained section of control (a) and gongjin-dan treatment group (b) at 14 days. ROI by F-18 FDG uptake was revealed as differential uptake rations (DURs) (c). Infarct volume was then analyzed using Meta-Morph program (d). In gongjin-dan treatment group, infarct volume was decreased compared to the control group. Also glucose uptake was increased significantly compared with control group. rCBF was significantly increased compared with control group (e). These effects were observed for 3, 4, 5, 8, 9, and 12 days after MCAo. Data are expressed as mean ± SD, *P < 0.05.
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fig2: Effect of gongjin-dan on infarct volume, glucose metabolism, and regional cerebral blood flow (rCBF) after MCAo. Images shown in the PET images and TTC-stained section of control (a) and gongjin-dan treatment group (b) at 14 days. ROI by F-18 FDG uptake was revealed as differential uptake rations (DURs) (c). Infarct volume was then analyzed using Meta-Morph program (d). In gongjin-dan treatment group, infarct volume was decreased compared to the control group. Also glucose uptake was increased significantly compared with control group. rCBF was significantly increased compared with control group (e). These effects were observed for 3, 4, 5, 8, 9, and 12 days after MCAo. Data are expressed as mean ± SD, *P < 0.05.

Mentions: The neuroprotective effect of gongjin-dan was evaluated by measuring the infarct volume at 14 days after MCAo. The brain of rats was stained with TTC to obtain the infarct volume and was calculated by measuring the area of infarct. Figure 2 is a photograph typical of TTC-stained sections of treatment and control groups. As seen in Figure 2, infarct volume of the gongjin-dan group was significantly decreased compared to the control (108.8 ± 42 versus 185 ± 43 mm3, P < 0.05) (Figure 2(d)). PET-CT has long been demonstrated as a marker of glucose metabolism in ischemia and infarction [25, 26]. In our studies, PET imaging data was interpreted in terms of DUR. With the contralateral side as baseline, the metabolic change of transient MCAo in an ipsilateral area was calculated by ROI value. As seen in Figure 2(a), the quantification of the ROI radioactivity was decreased in glucose uptake following MCAo. From these control data, a DUR in the ischemic region was significantly increased compared with normal. A DUR of gongjin-dan treatment was decreased compared to the control (11.6 ± 3.2 versus 17.4 ± 7.9%, P < 0.05). This data suggests that protected ischemic area by gongjin-dan could uptake glucose metabolism.


A Pilot Study for the Neuroprotective Effect of Gongjin-dan on Transient Middle Cerebral Artery Occlusion-Induced Ischemic Rat Brain.

Sunwoo YY, Park SI, Chung YA, Lee J, Park MS, Jang KS, Maeng LS, Jang DK, Im R, Jung YJ, Park SA, Kang ES, Kim MW, Han YM - Evid Based Complement Alternat Med (2012)

Effect of gongjin-dan on infarct volume, glucose metabolism, and regional cerebral blood flow (rCBF) after MCAo. Images shown in the PET images and TTC-stained section of control (a) and gongjin-dan treatment group (b) at 14 days. ROI by F-18 FDG uptake was revealed as differential uptake rations (DURs) (c). Infarct volume was then analyzed using Meta-Morph program (d). In gongjin-dan treatment group, infarct volume was decreased compared to the control group. Also glucose uptake was increased significantly compared with control group. rCBF was significantly increased compared with control group (e). These effects were observed for 3, 4, 5, 8, 9, and 12 days after MCAo. Data are expressed as mean ± SD, *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375177&req=5

fig2: Effect of gongjin-dan on infarct volume, glucose metabolism, and regional cerebral blood flow (rCBF) after MCAo. Images shown in the PET images and TTC-stained section of control (a) and gongjin-dan treatment group (b) at 14 days. ROI by F-18 FDG uptake was revealed as differential uptake rations (DURs) (c). Infarct volume was then analyzed using Meta-Morph program (d). In gongjin-dan treatment group, infarct volume was decreased compared to the control group. Also glucose uptake was increased significantly compared with control group. rCBF was significantly increased compared with control group (e). These effects were observed for 3, 4, 5, 8, 9, and 12 days after MCAo. Data are expressed as mean ± SD, *P < 0.05.
Mentions: The neuroprotective effect of gongjin-dan was evaluated by measuring the infarct volume at 14 days after MCAo. The brain of rats was stained with TTC to obtain the infarct volume and was calculated by measuring the area of infarct. Figure 2 is a photograph typical of TTC-stained sections of treatment and control groups. As seen in Figure 2, infarct volume of the gongjin-dan group was significantly decreased compared to the control (108.8 ± 42 versus 185 ± 43 mm3, P < 0.05) (Figure 2(d)). PET-CT has long been demonstrated as a marker of glucose metabolism in ischemia and infarction [25, 26]. In our studies, PET imaging data was interpreted in terms of DUR. With the contralateral side as baseline, the metabolic change of transient MCAo in an ipsilateral area was calculated by ROI value. As seen in Figure 2(a), the quantification of the ROI radioactivity was decreased in glucose uptake following MCAo. From these control data, a DUR in the ischemic region was significantly increased compared with normal. A DUR of gongjin-dan treatment was decreased compared to the control (11.6 ± 3.2 versus 17.4 ± 7.9%, P < 0.05). This data suggests that protected ischemic area by gongjin-dan could uptake glucose metabolism.

Bottom Line: Behavior tests demonstrated a significant improvement compared to the control.Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area.These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

View Article: PubMed Central - PubMed

Affiliation: Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon 403-720, Republic of Korea.

ABSTRACT
In this study, we investigated whether gongjin-dan improves functional recovery and has neuroprotective effects on reducing the infarct volume after transient middle cerebral artery occlusion (MCAo). Infarct volume was measured using TTC staining and glucose utilization by F-18 FDG PET. Functional improvement was evaluated with the Rota-rod, treadmill, Garcia score test, and adhesive removal test. At 14 days after MCAo, neuronal cell survival, astrocytes expansion, and apoptosis were assessed by immunohistofluorescence staining in the peri-infarct region. Also, the expression of neurotrophic factors and inflammatory cytokines such as VEGF, BDNF, Cox-2, TNF-α, IL-1β, and IL-1α was measured in ischemic hemisphere regions. The gongjin-dan-treated group showed both reduced infarct volume and increased glucose utilization. Behavior tests demonstrated a significant improvement compared to the control. Also in the gongjin-dan treated group, NeuN-positive cells were increased and number of astrocytes, microglia, and apoptotic cells was significantly decreased compared with the control group in the ischemic peri-infarct area. Furthermore, the expression of VEGF and BDNF was increased and level of Cox-2, TNF-α, IL-1β, and IL-1α was decreased. These results suggest that gongjin-dan may improve functional outcome through the rapid restoration of metabolism and can be considered as a potential neuroprotective agent.

No MeSH data available.


Related in: MedlinePlus