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Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP).

Mackintosh C, Clark G, Tolentino B, Liggett S, de Lisle G, Griffin F - Vet Med Int (2012)

Bottom Line: All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50.At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12-24 weeks.IFN-γ responses peaked at 8-15 weeks and were higher in mildly affected animals than in those with severe lesions.

View Article: PubMed Central - PubMed

Affiliation: AgResearch Invermay, P.O. Box 50034, Mosgiel, New Zealand.

ABSTRACT
Paratuberculosis progresses more quickly in young red deer than in sheep or cattle. This study describes the clinical, immunological and pathological changes over a 50-week period in fourteen 4-month-old red deer that received heavy oral challenge with Mycobacterium avium subsp. paratuberculosis (MAP). At 4 and 12 weeks post challenge they were anaesthetized and a section of jejunal lymph node was surgically removed for culture, histopathology, and genetic studies. All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50. Week 4 biopsies showed no paratuberculosis lesions, but MAP was cultured from all animals. At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12-24 weeks. IFN-γ responses peaked at 8-15 weeks and were higher in mildly affected animals than in those with severe lesions.

No MeSH data available.


Related in: MedlinePlus

Mean gamma interferon (IFN-γ) assay results in ELISA units (EUs) for the 14 deer sorted into three groups based on their total lesion severity score (LSS) at week 50 after MAP challenge at day 0.
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Related In: Results  -  Collection


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fig2: Mean gamma interferon (IFN-γ) assay results in ELISA units (EUs) for the 14 deer sorted into three groups based on their total lesion severity score (LSS) at week 50 after MAP challenge at day 0.

Mentions: The IFNγ of the least affected animals (total LSS 2–7) rose earlier and peaked higher than those of the more severely affected animals (total LSS 11-12) during the 50-week study (Figure 2). The IFNγ responses peaked twice, with the first peak at 12–15 weeks pc, a decline to a low at 24 weeks, and a second peak at 30–36 weeks. Only the LSS 2–7 group had an increase in IFNγ at Week 50, 2 weeks after the skin test.


Longitudinal Pathogenesis Study of Young Red Deer (Cervus elaphus) after Experimental Challenge with Mycobacterium avium subsp. paratuberculosis (MAP).

Mackintosh C, Clark G, Tolentino B, Liggett S, de Lisle G, Griffin F - Vet Med Int (2012)

Mean gamma interferon (IFN-γ) assay results in ELISA units (EUs) for the 14 deer sorted into three groups based on their total lesion severity score (LSS) at week 50 after MAP challenge at day 0.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375165&req=5

fig2: Mean gamma interferon (IFN-γ) assay results in ELISA units (EUs) for the 14 deer sorted into three groups based on their total lesion severity score (LSS) at week 50 after MAP challenge at day 0.
Mentions: The IFNγ of the least affected animals (total LSS 2–7) rose earlier and peaked higher than those of the more severely affected animals (total LSS 11-12) during the 50-week study (Figure 2). The IFNγ responses peaked twice, with the first peak at 12–15 weeks pc, a decline to a low at 24 weeks, and a second peak at 30–36 weeks. Only the LSS 2–7 group had an increase in IFNγ at Week 50, 2 weeks after the skin test.

Bottom Line: All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50.At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12-24 weeks.IFN-γ responses peaked at 8-15 weeks and were higher in mildly affected animals than in those with severe lesions.

View Article: PubMed Central - PubMed

Affiliation: AgResearch Invermay, P.O. Box 50034, Mosgiel, New Zealand.

ABSTRACT
Paratuberculosis progresses more quickly in young red deer than in sheep or cattle. This study describes the clinical, immunological and pathological changes over a 50-week period in fourteen 4-month-old red deer that received heavy oral challenge with Mycobacterium avium subsp. paratuberculosis (MAP). At 4 and 12 weeks post challenge they were anaesthetized and a section of jejunal lymph node was surgically removed for culture, histopathology, and genetic studies. All 14 deer became infected, none were clinically affected, and they had varying degrees of subclinical disease when killed at week 50. Week 4 biopsies showed no paratuberculosis lesions, but MAP was cultured from all animals. At weeks 12 and 50 histopathological lesions ranged from mild to severe with corresponding low-to-high antibody titres, which peaked at 12-24 weeks. IFN-γ responses peaked at 8-15 weeks and were higher in mildly affected animals than in those with severe lesions.

No MeSH data available.


Related in: MedlinePlus