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Physicochemical properties and cellular responses of strontium-doped gypsum biomaterials.

Pouria A, Bandegani H, Pourbaghi-Masouleh M, Hesaraki S, Alizadeh M - Bioinorg Chem Appl (2012)

Bottom Line: Gypsum was the only phase found in the composition of both pure and gypsum:Sr, meanwhile a shift into lower diffraction angles was observed in the X-ray diffraction patterns of doped specimens.Compared to pure gypsum, the osteoblasts cultured on strontium-doped samples showed better proliferation rate and higher alkaline phosphatase activity, depending on Sr concentration.These observations can predict better in vivo behavior of strontium-doped gypsum compared to pure one.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, P.O. Box 31787/316, Karaj 3177983634, Iran.

ABSTRACT
This paper describes some physical, structural, and biological properties of gypsum bioceramics doped with various amounts of strontium ions (0.19-2.23 wt%) and compares these properties with those of a pure gypsum as control. Strontium-doped gypsum (gypsum:Sr) was obtained by mixing calcium sulfate hemihydrate powder and solutions of strontium nitrate followed by washing the specimens with distilled water to remove residual salts. Gypsum was the only phase found in the composition of both pure and gypsum:Sr, meanwhile a shift into lower diffraction angles was observed in the X-ray diffraction patterns of doped specimens. Microstructure of all gypsum specimens consisted of many rod-like small crystals entangled to each other with more elongation and higher thickness in the case of gypsum:Sr. The Sr-doped sample exhibited higher compressive strength and lower solubility than pure gypsum. A continuous release of strontium ions was observed from the gypsum:Sr during soaking it in simulated body fluid for 14 days. Compared to pure gypsum, the osteoblasts cultured on strontium-doped samples showed better proliferation rate and higher alkaline phosphatase activity, depending on Sr concentration. These observations can predict better in vivo behavior of strontium-doped gypsum compared to pure one.

No MeSH data available.


Normalized ALP activity of G-292 osteoblastic cells cultured on gypsum specimens with various amounts of Sr dopant (*P < 0.05).
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fig11: Normalized ALP activity of G-292 osteoblastic cells cultured on gypsum specimens with various amounts of Sr dopant (*P < 0.05).

Mentions: Alkaline phosphatase is known as an early osteoblastic differentiation marker and is produced by the cells showing mineralized extracellular matrix [31]. Alkaline phosphatase activity of the osteoblasts is illustrated in Figure 11 as normalized absorbance number (per unit cell number) against the culture time. ALP activity of the osteoblasts cultured on gypsum specimens, especially G-Sr1, was better than those cultured on polystyrene. While higher ALP activity was found on G-Sr1 compared to gypsum, the difference in ALP value of pure gypsum and other Sr-doped gypsum specimens was not statistically significant (P > 0.05). Alkaline phosphatase activity was inhibited on day 7 for all specimens probably due to conflux of extended cell [16]. There are many studies that confirm this fact that strontium ions incorporated into composition of bioceramics can improve proliferation and/or alkaline phosphatase activity of osteoblastic cells [32]. In this study, it was shown that the presence of Sr2+ ions in the lattice structure of gypsum could also play important role in proliferation and ALP activity of G-292 osteoblastic cells.


Physicochemical properties and cellular responses of strontium-doped gypsum biomaterials.

Pouria A, Bandegani H, Pourbaghi-Masouleh M, Hesaraki S, Alizadeh M - Bioinorg Chem Appl (2012)

Normalized ALP activity of G-292 osteoblastic cells cultured on gypsum specimens with various amounts of Sr dopant (*P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375162&req=5

fig11: Normalized ALP activity of G-292 osteoblastic cells cultured on gypsum specimens with various amounts of Sr dopant (*P < 0.05).
Mentions: Alkaline phosphatase is known as an early osteoblastic differentiation marker and is produced by the cells showing mineralized extracellular matrix [31]. Alkaline phosphatase activity of the osteoblasts is illustrated in Figure 11 as normalized absorbance number (per unit cell number) against the culture time. ALP activity of the osteoblasts cultured on gypsum specimens, especially G-Sr1, was better than those cultured on polystyrene. While higher ALP activity was found on G-Sr1 compared to gypsum, the difference in ALP value of pure gypsum and other Sr-doped gypsum specimens was not statistically significant (P > 0.05). Alkaline phosphatase activity was inhibited on day 7 for all specimens probably due to conflux of extended cell [16]. There are many studies that confirm this fact that strontium ions incorporated into composition of bioceramics can improve proliferation and/or alkaline phosphatase activity of osteoblastic cells [32]. In this study, it was shown that the presence of Sr2+ ions in the lattice structure of gypsum could also play important role in proliferation and ALP activity of G-292 osteoblastic cells.

Bottom Line: Gypsum was the only phase found in the composition of both pure and gypsum:Sr, meanwhile a shift into lower diffraction angles was observed in the X-ray diffraction patterns of doped specimens.Compared to pure gypsum, the osteoblasts cultured on strontium-doped samples showed better proliferation rate and higher alkaline phosphatase activity, depending on Sr concentration.These observations can predict better in vivo behavior of strontium-doped gypsum compared to pure one.

View Article: PubMed Central - PubMed

Affiliation: Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, P.O. Box 31787/316, Karaj 3177983634, Iran.

ABSTRACT
This paper describes some physical, structural, and biological properties of gypsum bioceramics doped with various amounts of strontium ions (0.19-2.23 wt%) and compares these properties with those of a pure gypsum as control. Strontium-doped gypsum (gypsum:Sr) was obtained by mixing calcium sulfate hemihydrate powder and solutions of strontium nitrate followed by washing the specimens with distilled water to remove residual salts. Gypsum was the only phase found in the composition of both pure and gypsum:Sr, meanwhile a shift into lower diffraction angles was observed in the X-ray diffraction patterns of doped specimens. Microstructure of all gypsum specimens consisted of many rod-like small crystals entangled to each other with more elongation and higher thickness in the case of gypsum:Sr. The Sr-doped sample exhibited higher compressive strength and lower solubility than pure gypsum. A continuous release of strontium ions was observed from the gypsum:Sr during soaking it in simulated body fluid for 14 days. Compared to pure gypsum, the osteoblasts cultured on strontium-doped samples showed better proliferation rate and higher alkaline phosphatase activity, depending on Sr concentration. These observations can predict better in vivo behavior of strontium-doped gypsum compared to pure one.

No MeSH data available.