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Hematological- and Neurological-Expressed Sequence 1 Gene Products in Progenitor Cells during Newt Retinal Development.

Goto T, Tokunaga F, Hisatomi O - Stem Cells Int (2012)

Bottom Line: We found that hematological- and neurological-expressed sequence 1 (Hn1) gene was induced in depigmented retinal pigment epithelial (RPE) cells, and its expression was maintained at later stages of newt retinal regeneration.We also found that the expression of Hn1 gene was not induced in mouse after retinal removal.Our results showed that Hn1 gene can be useful for detection of undifferentiated and dedifferentiated cells during both newt retinal development and regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cho 1-1, Toyonaka 560-0043, Japan.

ABSTRACT
Urodele amphibians such as Japanese common newts have a remarkable ability to regenerate their injured neural retina, even as adults. We found that hematological- and neurological-expressed sequence 1 (Hn1) gene was induced in depigmented retinal pigment epithelial (RPE) cells, and its expression was maintained at later stages of newt retinal regeneration. In this study, we investigated the distribution of the HN1 protein, the product of the Hn1 gene, in the developing retinas. Our immunohistochemical analyses suggested that the HN1 protein was highly expressed in an immature retina, and the subcellular localization changed during this retinogenesis as observed in newt retinal regeneration. We also found that the expression of Hn1 gene was not induced in mouse after retinal removal. Our results showed that Hn1 gene can be useful for detection of undifferentiated and dedifferentiated cells during both newt retinal development and regeneration.

No MeSH data available.


RT-PCR analysis of mouse Hn1 gene after retinal removal. The upper panel shows the expression of mouse Hn1 gene, and the lower panel shows the expression of the β-actin gene as an internal control. Abbreviations indicated as follows: NR: normal retina from adult mice; RPE: retinal pigment epithelium from adult mice; days po: days after operation of retinal removal.
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fig4: RT-PCR analysis of mouse Hn1 gene after retinal removal. The upper panel shows the expression of mouse Hn1 gene, and the lower panel shows the expression of the β-actin gene as an internal control. Abbreviations indicated as follows: NR: normal retina from adult mice; RPE: retinal pigment epithelium from adult mice; days po: days after operation of retinal removal.

Mentions: We used mice as a model which lacked the ability of spontaneous regeneration. Four days after the removal of neural retinas from mouse eyes, most pigmented cell layers were attached to sclera, but some part of cell layers was detached, and pigmented cells were found inside of eye cavity (data not shown). The appearance of pigmented cell layers was similar at 18 days after operation. Our observation did not contradict the previous report that the retinectomy caused the migration of the epithelial cells, development of multiple layers with vacuoles in rabbit eyes [24]. We examined the expressions of Hn1 gene in mouse eyecups by RT-PCR (Figure 4). Hn1 mRNA was expressed in mouse normal retina and RPE cells. After retinal removal, the expression level of mouse Hn1 gene was almost equal, suggesting that mouse Hn1 gene did not induced the remaining RPE cells or others, unlike in the case of newt. The upregulation of Hn1 gene was possibly a unique phenomenon observed in dedifferentiating RPE cells.


Hematological- and Neurological-Expressed Sequence 1 Gene Products in Progenitor Cells during Newt Retinal Development.

Goto T, Tokunaga F, Hisatomi O - Stem Cells Int (2012)

RT-PCR analysis of mouse Hn1 gene after retinal removal. The upper panel shows the expression of mouse Hn1 gene, and the lower panel shows the expression of the β-actin gene as an internal control. Abbreviations indicated as follows: NR: normal retina from adult mice; RPE: retinal pigment epithelium from adult mice; days po: days after operation of retinal removal.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3375142&req=5

fig4: RT-PCR analysis of mouse Hn1 gene after retinal removal. The upper panel shows the expression of mouse Hn1 gene, and the lower panel shows the expression of the β-actin gene as an internal control. Abbreviations indicated as follows: NR: normal retina from adult mice; RPE: retinal pigment epithelium from adult mice; days po: days after operation of retinal removal.
Mentions: We used mice as a model which lacked the ability of spontaneous regeneration. Four days after the removal of neural retinas from mouse eyes, most pigmented cell layers were attached to sclera, but some part of cell layers was detached, and pigmented cells were found inside of eye cavity (data not shown). The appearance of pigmented cell layers was similar at 18 days after operation. Our observation did not contradict the previous report that the retinectomy caused the migration of the epithelial cells, development of multiple layers with vacuoles in rabbit eyes [24]. We examined the expressions of Hn1 gene in mouse eyecups by RT-PCR (Figure 4). Hn1 mRNA was expressed in mouse normal retina and RPE cells. After retinal removal, the expression level of mouse Hn1 gene was almost equal, suggesting that mouse Hn1 gene did not induced the remaining RPE cells or others, unlike in the case of newt. The upregulation of Hn1 gene was possibly a unique phenomenon observed in dedifferentiating RPE cells.

Bottom Line: We found that hematological- and neurological-expressed sequence 1 (Hn1) gene was induced in depigmented retinal pigment epithelial (RPE) cells, and its expression was maintained at later stages of newt retinal regeneration.We also found that the expression of Hn1 gene was not induced in mouse after retinal removal.Our results showed that Hn1 gene can be useful for detection of undifferentiated and dedifferentiated cells during both newt retinal development and regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Earth and Space Science, Graduate School of Science, Osaka University, Machikaneyama-cho 1-1, Toyonaka 560-0043, Japan.

ABSTRACT
Urodele amphibians such as Japanese common newts have a remarkable ability to regenerate their injured neural retina, even as adults. We found that hematological- and neurological-expressed sequence 1 (Hn1) gene was induced in depigmented retinal pigment epithelial (RPE) cells, and its expression was maintained at later stages of newt retinal regeneration. In this study, we investigated the distribution of the HN1 protein, the product of the Hn1 gene, in the developing retinas. Our immunohistochemical analyses suggested that the HN1 protein was highly expressed in an immature retina, and the subcellular localization changed during this retinogenesis as observed in newt retinal regeneration. We also found that the expression of Hn1 gene was not induced in mouse after retinal removal. Our results showed that Hn1 gene can be useful for detection of undifferentiated and dedifferentiated cells during both newt retinal development and regeneration.

No MeSH data available.