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The effects of new Alibernet red wine extract on nitric oxide and reactive oxygen species production in spontaneously hypertensive rats.

Kondrashov A, Vranková S, Dovinová I, Sevčík R, Parohová J, Barta A, Pecháňová O, Kovacsová M - Oxid Med Cell Longev (2012)

Bottom Line: Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine.In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR.Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, 121 08 Prague, Czech Republic. alexey.kondrasov@lf1.cuni.cz

ABSTRACT
We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE) and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day) for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

Show MeSH
The effect of Alibernet wine extract (AWE) on superoxide dismutase (SOD) activity (a) and SOD1 protein expression (b) in the left ventricle (LV) of Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). P < 0.05 as compared to untreated animals. WKY-C: control group; WKY + AWE: animals treated with the Alibernet wine extract; SHR-C: control group; SHR + AWE: animals treated with the Alibernet wine extract.
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Related In: Results  -  Collection


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fig4: The effect of Alibernet wine extract (AWE) on superoxide dismutase (SOD) activity (a) and SOD1 protein expression (b) in the left ventricle (LV) of Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). P < 0.05 as compared to untreated animals. WKY-C: control group; WKY + AWE: animals treated with the Alibernet wine extract; SHR-C: control group; SHR + AWE: animals treated with the Alibernet wine extract.

Mentions: The Alibernet wine extract treatment failed to affect SOD activities as well as SOD1 protein expressions in the tissues of WKY rats. On the other hand, AWE treatment significantly increased SOD activity in the LV of SHR (by 54%; Figure 4(a)). However, it had no effect on left ventricular SOD1 protein expression (Figure 4(b)). Similarly, AWE treatment failed to affect SOD activity and SOD1 protein expression in the aorta, kidney, and plasma of SHR (data not shown).


The effects of new Alibernet red wine extract on nitric oxide and reactive oxygen species production in spontaneously hypertensive rats.

Kondrashov A, Vranková S, Dovinová I, Sevčík R, Parohová J, Barta A, Pecháňová O, Kovacsová M - Oxid Med Cell Longev (2012)

The effect of Alibernet wine extract (AWE) on superoxide dismutase (SOD) activity (a) and SOD1 protein expression (b) in the left ventricle (LV) of Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). P < 0.05 as compared to untreated animals. WKY-C: control group; WKY + AWE: animals treated with the Alibernet wine extract; SHR-C: control group; SHR + AWE: animals treated with the Alibernet wine extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375118&req=5

fig4: The effect of Alibernet wine extract (AWE) on superoxide dismutase (SOD) activity (a) and SOD1 protein expression (b) in the left ventricle (LV) of Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR). P < 0.05 as compared to untreated animals. WKY-C: control group; WKY + AWE: animals treated with the Alibernet wine extract; SHR-C: control group; SHR + AWE: animals treated with the Alibernet wine extract.
Mentions: The Alibernet wine extract treatment failed to affect SOD activities as well as SOD1 protein expressions in the tissues of WKY rats. On the other hand, AWE treatment significantly increased SOD activity in the LV of SHR (by 54%; Figure 4(a)). However, it had no effect on left ventricular SOD1 protein expression (Figure 4(b)). Similarly, AWE treatment failed to affect SOD activity and SOD1 protein expression in the aorta, kidney, and plasma of SHR (data not shown).

Bottom Line: Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine.In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR.Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, 121 08 Prague, Czech Republic. alexey.kondrasov@lf1.cuni.cz

ABSTRACT
We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE) and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day) for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

Show MeSH