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Urinary TWEAK level as a marker of lupus nephritis activity in 46 cases.

Xuejing Z, Jiazhen T, Jun L, Xiangqing X, Shuguang Y, Fuyou L - J. Biomed. Biotechnol. (2012)

Bottom Line: Urinary TWEAK (uTWEAK) level and Monocyte chemoattractant protein-1 (MCP-1) level were detected by ELISA.Urinary TWEAK level was significantly higher in active LN group than in non-active LN group.Correlation analysis showed that urinary TWEAK levels were significantly correlated with activity index (r = 0.825, P < 0.01), glomerular activity index (r = 0.754, P < 0.01), and tubulointerstitial qualitative activity index (r = 0.751, P < 0.01), while not significant correlated with chronicity Index (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.

ABSTRACT

Objective: This study is designed to observe the urinary tumor necrosis factor-like weak inducer of apoptosis (TWEAK) levels in patients with lupus nephritis (LN) and to identify new biomarker of lupus nephritis activity.

Methods: Study subjects were 46 cases of patients with LN (including 34 of active cases) who underwent routine renal biopsy. Activity and chronicity indexes of LN were assessed using pathological criteria proposed by Hill et al. in 2000. Urinary TWEAK (uTWEAK) level and Monocyte chemoattractant protein-1 (MCP-1) level were detected by ELISA.

Results: Urinary TWEAK level was significantly higher in active LN group than in non-active LN group. Correlation analysis showed that urinary TWEAK levels were significantly correlated with activity index (r = 0.825, P < 0.01), glomerular activity index (r = 0.754, P < 0.01), and tubulointerstitial qualitative activity index (r = 0.751, P < 0.01), while not significant correlated with chronicity Index (P > 0.05). The association between urinary TWEAK levels and urinary MCP-1 levels were significant in active LN group (r = 0.809, P < 0.01) but not significant in non-active LN group (P > 0.05).

Conclusions: uWEAK levels were correlated with all active indexes of LN, suggesting its potential role as novel biomarker of active lupus nephritis.

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Related in: MedlinePlus

Features of the histopathology of active lupus nephritis. (a) Glomerular mesangial cells proliferation accompanied with cellular crescents (arrow showed). (HE staining, ×200). (b) Subepithelial immune deposits to form a “platinum ear” (arrow showed). (PASM staining, ×200). (c) Immune deposits in basement membrane. The “spikes bristle” can be observed in basement membrane (arrow showed). (PASM staining, ×1000). (d) Renal tubular epithelial cells shed accompanied with interstitial inflammatory infiltration (arrow showed). (HE staining, ×400). (e) Normal glomerulus without mesangial cells proliferation. (PAS staining, ×200). (f) Normal glomerulus without thick basement membrane. (PASM staining, ×1000).
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fig1: Features of the histopathology of active lupus nephritis. (a) Glomerular mesangial cells proliferation accompanied with cellular crescents (arrow showed). (HE staining, ×200). (b) Subepithelial immune deposits to form a “platinum ear” (arrow showed). (PASM staining, ×200). (c) Immune deposits in basement membrane. The “spikes bristle” can be observed in basement membrane (arrow showed). (PASM staining, ×1000). (d) Renal tubular epithelial cells shed accompanied with interstitial inflammatory infiltration (arrow showed). (HE staining, ×400). (e) Normal glomerulus without mesangial cells proliferation. (PAS staining, ×200). (f) Normal glomerulus without thick basement membrane. (PASM staining, ×1000).

Mentions: The pathological changes of active lupus nephritis contain glomerular mesangial cells proliferation, cellular crescents, thickening of capillary walls, karyorrhexis, hyaline thrombi, and interstitial inflammatory infiltration (Figure 1).


Urinary TWEAK level as a marker of lupus nephritis activity in 46 cases.

Xuejing Z, Jiazhen T, Jun L, Xiangqing X, Shuguang Y, Fuyou L - J. Biomed. Biotechnol. (2012)

Features of the histopathology of active lupus nephritis. (a) Glomerular mesangial cells proliferation accompanied with cellular crescents (arrow showed). (HE staining, ×200). (b) Subepithelial immune deposits to form a “platinum ear” (arrow showed). (PASM staining, ×200). (c) Immune deposits in basement membrane. The “spikes bristle” can be observed in basement membrane (arrow showed). (PASM staining, ×1000). (d) Renal tubular epithelial cells shed accompanied with interstitial inflammatory infiltration (arrow showed). (HE staining, ×400). (e) Normal glomerulus without mesangial cells proliferation. (PAS staining, ×200). (f) Normal glomerulus without thick basement membrane. (PASM staining, ×1000).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3375113&req=5

fig1: Features of the histopathology of active lupus nephritis. (a) Glomerular mesangial cells proliferation accompanied with cellular crescents (arrow showed). (HE staining, ×200). (b) Subepithelial immune deposits to form a “platinum ear” (arrow showed). (PASM staining, ×200). (c) Immune deposits in basement membrane. The “spikes bristle” can be observed in basement membrane (arrow showed). (PASM staining, ×1000). (d) Renal tubular epithelial cells shed accompanied with interstitial inflammatory infiltration (arrow showed). (HE staining, ×400). (e) Normal glomerulus without mesangial cells proliferation. (PAS staining, ×200). (f) Normal glomerulus without thick basement membrane. (PASM staining, ×1000).
Mentions: The pathological changes of active lupus nephritis contain glomerular mesangial cells proliferation, cellular crescents, thickening of capillary walls, karyorrhexis, hyaline thrombi, and interstitial inflammatory infiltration (Figure 1).

Bottom Line: Urinary TWEAK (uTWEAK) level and Monocyte chemoattractant protein-1 (MCP-1) level were detected by ELISA.Urinary TWEAK level was significantly higher in active LN group than in non-active LN group.Correlation analysis showed that urinary TWEAK levels were significantly correlated with activity index (r = 0.825, P < 0.01), glomerular activity index (r = 0.754, P < 0.01), and tubulointerstitial qualitative activity index (r = 0.751, P < 0.01), while not significant correlated with chronicity Index (P > 0.05).

View Article: PubMed Central - PubMed

Affiliation: Division of Nephrology, The Second Xiangya Hospital, Central South University, Changsha 410011, China.

ABSTRACT

Objective: This study is designed to observe the urinary tumor necrosis factor-like weak inducer of apoptosis (TWEAK) levels in patients with lupus nephritis (LN) and to identify new biomarker of lupus nephritis activity.

Methods: Study subjects were 46 cases of patients with LN (including 34 of active cases) who underwent routine renal biopsy. Activity and chronicity indexes of LN were assessed using pathological criteria proposed by Hill et al. in 2000. Urinary TWEAK (uTWEAK) level and Monocyte chemoattractant protein-1 (MCP-1) level were detected by ELISA.

Results: Urinary TWEAK level was significantly higher in active LN group than in non-active LN group. Correlation analysis showed that urinary TWEAK levels were significantly correlated with activity index (r = 0.825, P < 0.01), glomerular activity index (r = 0.754, P < 0.01), and tubulointerstitial qualitative activity index (r = 0.751, P < 0.01), while not significant correlated with chronicity Index (P > 0.05). The association between urinary TWEAK levels and urinary MCP-1 levels were significant in active LN group (r = 0.809, P < 0.01) but not significant in non-active LN group (P > 0.05).

Conclusions: uWEAK levels were correlated with all active indexes of LN, suggesting its potential role as novel biomarker of active lupus nephritis.

Show MeSH
Related in: MedlinePlus