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The Number of Endothelial Progenitor Cells is Decreased in Patients With Non-Dipper Hypertension.

Kim S, Kim NH, Kim YK, Yoo JH, Shin SN, Ko JS, Kim YK, Rhee SJ, Yun KH, Lee EM, Yoo NJ, Oh SK, Jeong JW - Korean Circ J (2012)

Bottom Line: A reduced number of EPCs and the functional activity have been associated with several cardiovascular risk factors.The circulating EPCs were statistically reduced in the non-dipper patients as compared to the dippers (104±60 vs. 66±47 EPCs per 106 mononuclear cells, p=0.027).The present study demonstrated that the EPC count was reduced in the peripheral bloodstream in non-dipper hypertensive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Center, Regional Cardiocerebrovascular Disease Center, Wonkwang University Hospital, Iksan, Korea.

ABSTRACT

Background and objectives: Circulating endothelial progenitor cells (EPCs) play a key role in the maintenance of endothelial homeostasis and promote vascular repair. A reduced number of EPCs and the functional activity have been associated with several cardiovascular risk factors. However, the relationship between the number of EPCs and circadian rhythm of the blood pressure (BP) remains unclear. The purpose of the present study was to evaluate the relationship between the circadian rhythm of the BP and EPCs in patients with essential hypertension.

Subjects and methods: A total of 45 patients with essential hypertension who were newly identified by outpatient BP measurements, underwent 24-hour ambulatory BP monitoring. Among the 45 patients with essential hypertension, 20 were classified as dippers (12 men and 8 women; mean age 48±14 years) and 25 as non-dippers (14 men and 11 women; mean age 52±18 years). The EPC count was isolated from the peripheral bloodstream and quantified by flow cytometry.

Results: The baseline clinical characteristics were similar between the dipper and non-dipper hypertensive patients. The circulating EPCs were statistically reduced in the non-dipper patients as compared to the dippers (104±60 vs. 66±47 EPCs per 106 mononuclear cells, p=0.027). The circulating EPC level correlated positively with the circadian changes in the systolic and diastolic BP (r=0.435, p=0.003, and r=0.310, p=0.038, respectively).

Conclusion: The present study demonstrated that the EPC count was reduced in the peripheral bloodstream in non-dipper hypertensive patients.

No MeSH data available.


Related in: MedlinePlus

Quantification of EPCs by flow cytometry. Circulating EPCs were identified by flow cytometry with low cytoplasmic granularity (R1) and with expression of cell surface antigens such as CD45lowCD34+ VEGFR2+. Arrows indicate CD45lowCD34+ VEGFR2+ cells. EPCs: endothelial progenitor cells, VEGFR2: vascular endothelial growth factor receptor 2, SSC: side scather, FITC: fluorescein isothiocyanate.
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Figure 1: Quantification of EPCs by flow cytometry. Circulating EPCs were identified by flow cytometry with low cytoplasmic granularity (R1) and with expression of cell surface antigens such as CD45lowCD34+ VEGFR2+. Arrows indicate CD45lowCD34+ VEGFR2+ cells. EPCs: endothelial progenitor cells, VEGFR2: vascular endothelial growth factor receptor 2, SSC: side scather, FITC: fluorescein isothiocyanate.

Mentions: In all participants, the total EPC count was assessed by using an in vitro assay, as described previously.8) In brief, mononuclear cells (MNCs) were obtained from peripheral blood samples (100 µL), and the EPCs were identified by flow cytometry (BD Biosciences, San Jose, CA, USA). Their phenotype was determined by immunohistochemistry after staining with 20 µL fluorescein PE-Cy5-conjugated anti-CD45 monoclonal antibody (Dynona, Korea), 20 µL of fluorescein isothiocyanate (FITC)-conjugated anti-CD34 monoclonal antibody (Dynona, Korea) and 10 µL of PE-conjugated anti-vascular endothelial growth factor receptor 2 (VEGFR2) monoclonal antibody (R&D, Minneapolis, MN, USA), and further incubated in a dark room for 1 hour. After appropriate gating with low cytoplasmic granularity and a low expression of CD45, the number of CD34+VEGFR2+ cells was quantified and expressed as the absolute number of cells per 1×106 peripheral MNCs. The total number of CD45lowCD34+ VEGFR2+ cells was then counted (Fig. 1).


The Number of Endothelial Progenitor Cells is Decreased in Patients With Non-Dipper Hypertension.

Kim S, Kim NH, Kim YK, Yoo JH, Shin SN, Ko JS, Kim YK, Rhee SJ, Yun KH, Lee EM, Yoo NJ, Oh SK, Jeong JW - Korean Circ J (2012)

Quantification of EPCs by flow cytometry. Circulating EPCs were identified by flow cytometry with low cytoplasmic granularity (R1) and with expression of cell surface antigens such as CD45lowCD34+ VEGFR2+. Arrows indicate CD45lowCD34+ VEGFR2+ cells. EPCs: endothelial progenitor cells, VEGFR2: vascular endothelial growth factor receptor 2, SSC: side scather, FITC: fluorescein isothiocyanate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369964&req=5

Figure 1: Quantification of EPCs by flow cytometry. Circulating EPCs were identified by flow cytometry with low cytoplasmic granularity (R1) and with expression of cell surface antigens such as CD45lowCD34+ VEGFR2+. Arrows indicate CD45lowCD34+ VEGFR2+ cells. EPCs: endothelial progenitor cells, VEGFR2: vascular endothelial growth factor receptor 2, SSC: side scather, FITC: fluorescein isothiocyanate.
Mentions: In all participants, the total EPC count was assessed by using an in vitro assay, as described previously.8) In brief, mononuclear cells (MNCs) were obtained from peripheral blood samples (100 µL), and the EPCs were identified by flow cytometry (BD Biosciences, San Jose, CA, USA). Their phenotype was determined by immunohistochemistry after staining with 20 µL fluorescein PE-Cy5-conjugated anti-CD45 monoclonal antibody (Dynona, Korea), 20 µL of fluorescein isothiocyanate (FITC)-conjugated anti-CD34 monoclonal antibody (Dynona, Korea) and 10 µL of PE-conjugated anti-vascular endothelial growth factor receptor 2 (VEGFR2) monoclonal antibody (R&D, Minneapolis, MN, USA), and further incubated in a dark room for 1 hour. After appropriate gating with low cytoplasmic granularity and a low expression of CD45, the number of CD34+VEGFR2+ cells was quantified and expressed as the absolute number of cells per 1×106 peripheral MNCs. The total number of CD45lowCD34+ VEGFR2+ cells was then counted (Fig. 1).

Bottom Line: A reduced number of EPCs and the functional activity have been associated with several cardiovascular risk factors.The circulating EPCs were statistically reduced in the non-dipper patients as compared to the dippers (104±60 vs. 66±47 EPCs per 106 mononuclear cells, p=0.027).The present study demonstrated that the EPC count was reduced in the peripheral bloodstream in non-dipper hypertensive patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Center, Regional Cardiocerebrovascular Disease Center, Wonkwang University Hospital, Iksan, Korea.

ABSTRACT

Background and objectives: Circulating endothelial progenitor cells (EPCs) play a key role in the maintenance of endothelial homeostasis and promote vascular repair. A reduced number of EPCs and the functional activity have been associated with several cardiovascular risk factors. However, the relationship between the number of EPCs and circadian rhythm of the blood pressure (BP) remains unclear. The purpose of the present study was to evaluate the relationship between the circadian rhythm of the BP and EPCs in patients with essential hypertension.

Subjects and methods: A total of 45 patients with essential hypertension who were newly identified by outpatient BP measurements, underwent 24-hour ambulatory BP monitoring. Among the 45 patients with essential hypertension, 20 were classified as dippers (12 men and 8 women; mean age 48±14 years) and 25 as non-dippers (14 men and 11 women; mean age 52±18 years). The EPC count was isolated from the peripheral bloodstream and quantified by flow cytometry.

Results: The baseline clinical characteristics were similar between the dipper and non-dipper hypertensive patients. The circulating EPCs were statistically reduced in the non-dipper patients as compared to the dippers (104±60 vs. 66±47 EPCs per 106 mononuclear cells, p=0.027). The circulating EPC level correlated positively with the circadian changes in the systolic and diastolic BP (r=0.435, p=0.003, and r=0.310, p=0.038, respectively).

Conclusion: The present study demonstrated that the EPC count was reduced in the peripheral bloodstream in non-dipper hypertensive patients.

No MeSH data available.


Related in: MedlinePlus