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Field and laboratory studies of the susceptibility of the green treefrog (Hyla cinerea) to Batrachochytrium dendrobatidis infection.

Brannelly LA, Chatfield MW, Richards-Zawacki CL - PLoS ONE (2012)

Bottom Line: Although we were able to infect H. cinerea with Bd in the lab, we did not observe any clinical signs of chytridiomycosis.Furthermore, infection by Bd does not appear to negatively affect body condition or growth rate of post-metamorphic individuals.Our results suggest that H. cinerea is not susceptible to chytridiomycosis post-metamorphosis and probably is not an important carrier of the fungal pathogen Bd in the southeastern United States, although susceptibility at the larval stage remains unknown.

View Article: PubMed Central - PubMed

Affiliation: Ecology and Evolutionary Biology Department, Tulane University, New Orleans, Louisiana, United States of America. lbrannel@tulane.edu

ABSTRACT
Amphibians worldwide are experiencing devastating declines, some of which are due to the amphibian chytrid fungus (Batrachochytrium dendrobatidis, Bd). Populations in the southeastern United States, however, have not been noticeably affected by the pathogen. The green treefrog (Hyla cinerea) is abundant and widespread in the southeastern United States, but has not been documented to harbor Bd infection. This study examined the susceptibility of H. cinerea to two strains of Bd in the lab and the prevalence of infection in wild populations of this species in southeastern Louisiana. Although we were able to infect H. cinerea with Bd in the lab, we did not observe any clinical signs of chytridiomycosis. Furthermore, infection by Bd does not appear to negatively affect body condition or growth rate of post-metamorphic individuals. We found no evidence of infection in surveys of wild H. cinerea. Our results suggest that H. cinerea is not susceptible to chytridiomycosis post-metamorphosis and probably is not an important carrier of the fungal pathogen Bd in the southeastern United States, although susceptibility at the larval stage remains unknown.

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Average zoospore equivalents over time.JEL423 infected individuals: black. SRS810 infected individuals: grey. Week indicates the number of weeks after last inoculation. Error bars are ± one standard error of the mean.
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pone-0038473-g005: Average zoospore equivalents over time.JEL423 infected individuals: black. SRS810 infected individuals: grey. Week indicates the number of weeks after last inoculation. Error bars are ± one standard error of the mean.

Mentions: The zoospore load was light for most of the animals throughout the experiment. While animals inoculated with JEL423 appeared to have higher zoospore loads near the end of the study, the pattern of variation did not differ among the two strains over the entire 26 weeks of the study (ANCOVA: F1,18 = 0.002, p = 0.965, see Fig. 5). Because they had very light infections, several animals tested negative for Bd, often times for several weeks, between positive swabs. Throughout the experiment, no animals showed clinical signs of chytridiomycosis.


Field and laboratory studies of the susceptibility of the green treefrog (Hyla cinerea) to Batrachochytrium dendrobatidis infection.

Brannelly LA, Chatfield MW, Richards-Zawacki CL - PLoS ONE (2012)

Average zoospore equivalents over time.JEL423 infected individuals: black. SRS810 infected individuals: grey. Week indicates the number of weeks after last inoculation. Error bars are ± one standard error of the mean.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369911&req=5

pone-0038473-g005: Average zoospore equivalents over time.JEL423 infected individuals: black. SRS810 infected individuals: grey. Week indicates the number of weeks after last inoculation. Error bars are ± one standard error of the mean.
Mentions: The zoospore load was light for most of the animals throughout the experiment. While animals inoculated with JEL423 appeared to have higher zoospore loads near the end of the study, the pattern of variation did not differ among the two strains over the entire 26 weeks of the study (ANCOVA: F1,18 = 0.002, p = 0.965, see Fig. 5). Because they had very light infections, several animals tested negative for Bd, often times for several weeks, between positive swabs. Throughout the experiment, no animals showed clinical signs of chytridiomycosis.

Bottom Line: Although we were able to infect H. cinerea with Bd in the lab, we did not observe any clinical signs of chytridiomycosis.Furthermore, infection by Bd does not appear to negatively affect body condition or growth rate of post-metamorphic individuals.Our results suggest that H. cinerea is not susceptible to chytridiomycosis post-metamorphosis and probably is not an important carrier of the fungal pathogen Bd in the southeastern United States, although susceptibility at the larval stage remains unknown.

View Article: PubMed Central - PubMed

Affiliation: Ecology and Evolutionary Biology Department, Tulane University, New Orleans, Louisiana, United States of America. lbrannel@tulane.edu

ABSTRACT
Amphibians worldwide are experiencing devastating declines, some of which are due to the amphibian chytrid fungus (Batrachochytrium dendrobatidis, Bd). Populations in the southeastern United States, however, have not been noticeably affected by the pathogen. The green treefrog (Hyla cinerea) is abundant and widespread in the southeastern United States, but has not been documented to harbor Bd infection. This study examined the susceptibility of H. cinerea to two strains of Bd in the lab and the prevalence of infection in wild populations of this species in southeastern Louisiana. Although we were able to infect H. cinerea with Bd in the lab, we did not observe any clinical signs of chytridiomycosis. Furthermore, infection by Bd does not appear to negatively affect body condition or growth rate of post-metamorphic individuals. We found no evidence of infection in surveys of wild H. cinerea. Our results suggest that H. cinerea is not susceptible to chytridiomycosis post-metamorphosis and probably is not an important carrier of the fungal pathogen Bd in the southeastern United States, although susceptibility at the larval stage remains unknown.

Show MeSH
Related in: MedlinePlus