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Elastogenic protein expression of a highly elastic murine spinal ligament: the ligamentum flavum.

Brown JP, Lind RM, Burzesi AF, Kuo CK - PLoS ONE (2012)

Bottom Line: We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth.These expression patterns correlated with reported skeletal and behavioral changes during murine development.This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, United States of America.

ABSTRACT
Spinal ligaments, such as the ligamentum flavum (LF), are prone to degeneration and iatrogenic injury that can lead to back pain and nerve dysfunction. Repair and regeneration strategies for these tissues are lacking, perhaps due to limited understanding of spinal ligament formation, the elaboration of its elastic fibers, maturation and homeostasis. Using immunohistochemistry and histology, we investigated murine LF elastogenesis and tissue formation from embryonic to mature postnatal stages. We characterized the spatiotemporal distribution of the key elastogenic proteins tropoelastin, fibrillin-1, fibulin-4 and lysyl oxidase. We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth. Elastic fibers were first detected histologically at postnatal day (P) 7, the earliest stage at which tropoelastin and fibulin-4 stained intensely. From P7 to P28, elastic fibers grew in diameter and became straighter along the axis. The growth of elastic fibers coincided with intense staining of tropoelastin and fibulin-4 staining, possibly supporting a chaperone role for fibulin-4. These expression patterns correlated with reported skeletal and behavioral changes during murine development. This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

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Fibulin-4 immunostaining of LF during development, maturation and aging.Fibulin-4 staining of LF (arrows point just inside edge of tissue) was high in intensity from P7 through P21, and was less intense at all other ages studied. Scale bar: 50 µm.
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pone-0038475-g006: Fibulin-4 immunostaining of LF during development, maturation and aging.Fibulin-4 staining of LF (arrows point just inside edge of tissue) was high in intensity from P7 through P21, and was less intense at all other ages studied. Scale bar: 50 µm.

Mentions: Fbln-4 staining intensity in the LF was greatest at P14, though moderately strong at P7 and P21 (Fig. 6). Before P7 and after P28, staining was detectable but relatively minimal.


Elastogenic protein expression of a highly elastic murine spinal ligament: the ligamentum flavum.

Brown JP, Lind RM, Burzesi AF, Kuo CK - PLoS ONE (2012)

Fibulin-4 immunostaining of LF during development, maturation and aging.Fibulin-4 staining of LF (arrows point just inside edge of tissue) was high in intensity from P7 through P21, and was less intense at all other ages studied. Scale bar: 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369910&req=5

pone-0038475-g006: Fibulin-4 immunostaining of LF during development, maturation and aging.Fibulin-4 staining of LF (arrows point just inside edge of tissue) was high in intensity from P7 through P21, and was less intense at all other ages studied. Scale bar: 50 µm.
Mentions: Fbln-4 staining intensity in the LF was greatest at P14, though moderately strong at P7 and P21 (Fig. 6). Before P7 and after P28, staining was detectable but relatively minimal.

Bottom Line: We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth.These expression patterns correlated with reported skeletal and behavioral changes during murine development.This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, United States of America.

ABSTRACT
Spinal ligaments, such as the ligamentum flavum (LF), are prone to degeneration and iatrogenic injury that can lead to back pain and nerve dysfunction. Repair and regeneration strategies for these tissues are lacking, perhaps due to limited understanding of spinal ligament formation, the elaboration of its elastic fibers, maturation and homeostasis. Using immunohistochemistry and histology, we investigated murine LF elastogenesis and tissue formation from embryonic to mature postnatal stages. We characterized the spatiotemporal distribution of the key elastogenic proteins tropoelastin, fibrillin-1, fibulin-4 and lysyl oxidase. We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth. Elastic fibers were first detected histologically at postnatal day (P) 7, the earliest stage at which tropoelastin and fibulin-4 stained intensely. From P7 to P28, elastic fibers grew in diameter and became straighter along the axis. The growth of elastic fibers coincided with intense staining of tropoelastin and fibulin-4 staining, possibly supporting a chaperone role for fibulin-4. These expression patterns correlated with reported skeletal and behavioral changes during murine development. This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

Show MeSH
Related in: MedlinePlus