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Elastogenic protein expression of a highly elastic murine spinal ligament: the ligamentum flavum.

Brown JP, Lind RM, Burzesi AF, Kuo CK - PLoS ONE (2012)

Bottom Line: We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth.These expression patterns correlated with reported skeletal and behavioral changes during murine development.This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, United States of America.

ABSTRACT
Spinal ligaments, such as the ligamentum flavum (LF), are prone to degeneration and iatrogenic injury that can lead to back pain and nerve dysfunction. Repair and regeneration strategies for these tissues are lacking, perhaps due to limited understanding of spinal ligament formation, the elaboration of its elastic fibers, maturation and homeostasis. Using immunohistochemistry and histology, we investigated murine LF elastogenesis and tissue formation from embryonic to mature postnatal stages. We characterized the spatiotemporal distribution of the key elastogenic proteins tropoelastin, fibrillin-1, fibulin-4 and lysyl oxidase. We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth. Elastic fibers were first detected histologically at postnatal day (P) 7, the earliest stage at which tropoelastin and fibulin-4 stained intensely. From P7 to P28, elastic fibers grew in diameter and became straighter along the axis. The growth of elastic fibers coincided with intense staining of tropoelastin and fibulin-4 staining, possibly supporting a chaperone role for fibulin-4. These expression patterns correlated with reported skeletal and behavioral changes during murine development. This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

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Tropoelastin immunostaining of LF during development, maturation and aging.Tropoelastin staining of LF (arrows point just inside edge of tissue) increased in intensity significantly first at P7 and remained high until P28. Staining intensity gradually decreased after P28 to a consistently low coloration that persisted throughout adulthood. Scale bar: 50 µm.
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pone-0038475-g004: Tropoelastin immunostaining of LF during development, maturation and aging.Tropoelastin staining of LF (arrows point just inside edge of tissue) increased in intensity significantly first at P7 and remained high until P28. Staining intensity gradually decreased after P28 to a consistently low coloration that persisted throughout adulthood. Scale bar: 50 µm.

Mentions: Tropoelastin staining was relatively weak in the LF from E15 through P0, with the exception of moderate staining intensity at E16 (Fig. 4). P7 was the first stage that staining intensity was observed to be substantially stronger, indicating a dramatic increase in tropoelastin production near this stage. Tropoelastin staining intensity remained relatively high until P35, when the staining intensity began to decrease. At P56 and P2-yrs, tropoelastin staining appeared minimal.


Elastogenic protein expression of a highly elastic murine spinal ligament: the ligamentum flavum.

Brown JP, Lind RM, Burzesi AF, Kuo CK - PLoS ONE (2012)

Tropoelastin immunostaining of LF during development, maturation and aging.Tropoelastin staining of LF (arrows point just inside edge of tissue) increased in intensity significantly first at P7 and remained high until P28. Staining intensity gradually decreased after P28 to a consistently low coloration that persisted throughout adulthood. Scale bar: 50 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369910&req=5

pone-0038475-g004: Tropoelastin immunostaining of LF during development, maturation and aging.Tropoelastin staining of LF (arrows point just inside edge of tissue) increased in intensity significantly first at P7 and remained high until P28. Staining intensity gradually decreased after P28 to a consistently low coloration that persisted throughout adulthood. Scale bar: 50 µm.
Mentions: Tropoelastin staining was relatively weak in the LF from E15 through P0, with the exception of moderate staining intensity at E16 (Fig. 4). P7 was the first stage that staining intensity was observed to be substantially stronger, indicating a dramatic increase in tropoelastin production near this stage. Tropoelastin staining intensity remained relatively high until P35, when the staining intensity began to decrease. At P56 and P2-yrs, tropoelastin staining appeared minimal.

Bottom Line: We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth.These expression patterns correlated with reported skeletal and behavioral changes during murine development.This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, United States of America.

ABSTRACT
Spinal ligaments, such as the ligamentum flavum (LF), are prone to degeneration and iatrogenic injury that can lead to back pain and nerve dysfunction. Repair and regeneration strategies for these tissues are lacking, perhaps due to limited understanding of spinal ligament formation, the elaboration of its elastic fibers, maturation and homeostasis. Using immunohistochemistry and histology, we investigated murine LF elastogenesis and tissue formation from embryonic to mature postnatal stages. We characterized the spatiotemporal distribution of the key elastogenic proteins tropoelastin, fibrillin-1, fibulin-4 and lysyl oxidase. We found that elastogenesis begins in utero with the microfibril constituent fibrillin-1 staining intensely just before birth. Elastic fibers were first detected histologically at postnatal day (P) 7, the earliest stage at which tropoelastin and fibulin-4 stained intensely. From P7 to P28, elastic fibers grew in diameter and became straighter along the axis. The growth of elastic fibers coincided with intense staining of tropoelastin and fibulin-4 staining, possibly supporting a chaperone role for fibulin-4. These expression patterns correlated with reported skeletal and behavioral changes during murine development. This immunohistochemical characterization of elastogenesis of the LF will be useful for future studies investigating mechanisms for elastogenesis and developing new strategies for treatment or regeneration of spinal ligaments and other highly elastic tissues.

Show MeSH
Related in: MedlinePlus