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Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

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Histological evaluation of the in vivo implanted scaffolds.Toluidine Blue, Acid Fucsin and Fast Green staining shows similar histocompatibility for both scaffolds 4 weeks after implantation (n = 6). A, B) Control, C, D) HA/Mgn 90/10. Scale bars: A, C) 1 mm, B, D) 500 µm.
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pone-0038710-g006: Histological evaluation of the in vivo implanted scaffolds.Toluidine Blue, Acid Fucsin and Fast Green staining shows similar histocompatibility for both scaffolds 4 weeks after implantation (n = 6). A, B) Control, C, D) HA/Mgn 90/10. Scale bars: A, C) 1 mm, B, D) 500 µm.

Mentions: At 4 weeks post-implantation, macroscopic evaluation showed the HA porous implants to be in the proper position and there was no evidence of haematoma, oedema, infection or tissue necrosis in either bone and peri-implant soft tissue associated with control or magnetic implants. Bone tissue was well visible around and inside the scaffold in both groups (Fig. 6A, C). Due to the interconnected porous structure, bone regenerated into the magnetic scaffold and after only 4 weeks some pores were completely full of new bone proving a good level of histocompatibility of the scaffold comparable to the control group (Fig. 6B, D).


Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Histological evaluation of the in vivo implanted scaffolds.Toluidine Blue, Acid Fucsin and Fast Green staining shows similar histocompatibility for both scaffolds 4 weeks after implantation (n = 6). A, B) Control, C, D) HA/Mgn 90/10. Scale bars: A, C) 1 mm, B, D) 500 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369900&req=5

pone-0038710-g006: Histological evaluation of the in vivo implanted scaffolds.Toluidine Blue, Acid Fucsin and Fast Green staining shows similar histocompatibility for both scaffolds 4 weeks after implantation (n = 6). A, B) Control, C, D) HA/Mgn 90/10. Scale bars: A, C) 1 mm, B, D) 500 µm.
Mentions: At 4 weeks post-implantation, macroscopic evaluation showed the HA porous implants to be in the proper position and there was no evidence of haematoma, oedema, infection or tissue necrosis in either bone and peri-implant soft tissue associated with control or magnetic implants. Bone tissue was well visible around and inside the scaffold in both groups (Fig. 6A, C). Due to the interconnected porous structure, bone regenerated into the magnetic scaffold and after only 4 weeks some pores were completely full of new bone proving a good level of histocompatibility of the scaffold comparable to the control group (Fig. 6B, D).

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

Show MeSH
Related in: MedlinePlus