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Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

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Analysis of cell viability.Cell viability was analysed by the Live/Dead assay (n = 3). Live-Dye stains for live cells in green, Propidium Iodide stains for dead cells in red. A) Control at day 7. B) HA/Mgn 95/5 at day 7. C) HA/Mgn 90/10 at day 7. D) HA/Mgn 50/50 at day 7. E) HA/Mgn 95/5 at day 14. F) HA/Mgn 95/5 at day 14. G) HA/Mgn 50/50 at day 14 with applied magnetic field. Scale bars: A–D) 500 µm. E) 250 µm. F, G) 100 µm.
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pone-0038710-g003: Analysis of cell viability.Cell viability was analysed by the Live/Dead assay (n = 3). Live-Dye stains for live cells in green, Propidium Iodide stains for dead cells in red. A) Control at day 7. B) HA/Mgn 95/5 at day 7. C) HA/Mgn 90/10 at day 7. D) HA/Mgn 50/50 at day 7. E) HA/Mgn 95/5 at day 14. F) HA/Mgn 95/5 at day 14. G) HA/Mgn 50/50 at day 14 with applied magnetic field. Scale bars: A–D) 500 µm. E) 250 µm. F, G) 100 µm.

Mentions: The Live/Dead assay showed a very high ratio of viable cells at each experimental time point, with a range between 73.9±4.3% and 92.8±3.0% and no differences between groups. No significant effect of magnetic field application was observed. After 7 days of culture, cells cover nearly the entire upper scaffold surface (Fig. 3A–D). Cells grew into the porous scaffold structure and infiltrated the scaffolds as shown in Fig. 3E. The very small ratio of dead cells found on magnetic materials was very similar to that found in the HA group for all time points (Fig. 3A–E). We analyzed also the inner surface after cutting the samples. After 14 days, cells had infiltrated into the scaffold and a high number of live cells were seen in all groups (Fig. 3F, G).


Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Analysis of cell viability.Cell viability was analysed by the Live/Dead assay (n = 3). Live-Dye stains for live cells in green, Propidium Iodide stains for dead cells in red. A) Control at day 7. B) HA/Mgn 95/5 at day 7. C) HA/Mgn 90/10 at day 7. D) HA/Mgn 50/50 at day 7. E) HA/Mgn 95/5 at day 14. F) HA/Mgn 95/5 at day 14. G) HA/Mgn 50/50 at day 14 with applied magnetic field. Scale bars: A–D) 500 µm. E) 250 µm. F, G) 100 µm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369900&req=5

pone-0038710-g003: Analysis of cell viability.Cell viability was analysed by the Live/Dead assay (n = 3). Live-Dye stains for live cells in green, Propidium Iodide stains for dead cells in red. A) Control at day 7. B) HA/Mgn 95/5 at day 7. C) HA/Mgn 90/10 at day 7. D) HA/Mgn 50/50 at day 7. E) HA/Mgn 95/5 at day 14. F) HA/Mgn 95/5 at day 14. G) HA/Mgn 50/50 at day 14 with applied magnetic field. Scale bars: A–D) 500 µm. E) 250 µm. F, G) 100 µm.
Mentions: The Live/Dead assay showed a very high ratio of viable cells at each experimental time point, with a range between 73.9±4.3% and 92.8±3.0% and no differences between groups. No significant effect of magnetic field application was observed. After 7 days of culture, cells cover nearly the entire upper scaffold surface (Fig. 3A–D). Cells grew into the porous scaffold structure and infiltrated the scaffolds as shown in Fig. 3E. The very small ratio of dead cells found on magnetic materials was very similar to that found in the HA group for all time points (Fig. 3A–E). We analyzed also the inner surface after cutting the samples. After 14 days, cells had infiltrated into the scaffold and a high number of live cells were seen in all groups (Fig. 3F, G).

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

Show MeSH
Related in: MedlinePlus