Limits...
Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

Show MeSH

Related in: MedlinePlus

Cell proliferation assay.The Picogreen DNA content assay was performed on cultures of osteoblast-like cells seeded on different HA/Mgn scaffolds at 7, 14 and 21 days of culture, either in the presence or absence of a magnetic field (MF) (n = 5). HA porous scaffold is used as control group. * p≤0.05.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3369900&req=5

pone-0038710-g001: Cell proliferation assay.The Picogreen DNA content assay was performed on cultures of osteoblast-like cells seeded on different HA/Mgn scaffolds at 7, 14 and 21 days of culture, either in the presence or absence of a magnetic field (MF) (n = 5). HA porous scaffold is used as control group. * p≤0.05.

Mentions: We have observed an overall increase in cell proliferation from day 7 to day 21 for all groups. Moreover, we have found a statistical significant increase in cells seeded on the HA/Mgn 90/10 after 7 days, both with or without a magnetic field (Fig. 1). At day 14, the group HA/Mgn 90/10 continued to have higher cell proliferation respect to the other groups, with this trend maintained until the end of the experiment (Fig. 1).


Magnetic hydroxyapatite bone substitutes to enhance tissue regeneration: evaluation in vitro using osteoblast-like cells and in vivo in a bone defect.

Panseri S, Cunha C, D'Alessandro T, Sandri M, Russo A, Giavaresi G, Marcacci M, Hung CT, Tampieri A - PLoS ONE (2012)

Cell proliferation assay.The Picogreen DNA content assay was performed on cultures of osteoblast-like cells seeded on different HA/Mgn scaffolds at 7, 14 and 21 days of culture, either in the presence or absence of a magnetic field (MF) (n = 5). HA porous scaffold is used as control group. * p≤0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369900&req=5

pone-0038710-g001: Cell proliferation assay.The Picogreen DNA content assay was performed on cultures of osteoblast-like cells seeded on different HA/Mgn scaffolds at 7, 14 and 21 days of culture, either in the presence or absence of a magnetic field (MF) (n = 5). HA porous scaffold is used as control group. * p≤0.05.
Mentions: We have observed an overall increase in cell proliferation from day 7 to day 21 for all groups. Moreover, we have found a statistical significant increase in cells seeded on the HA/Mgn 90/10 after 7 days, both with or without a magnetic field (Fig. 1). At day 14, the group HA/Mgn 90/10 continued to have higher cell proliferation respect to the other groups, with this trend maintained until the end of the experiment (Fig. 1).

Bottom Line: Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field.HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage.Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biomechanics and Technology Innovation, Rizzoli Orthopaedic Institute, Bologna, Italy. s.panseri@biomec.ior.it

ABSTRACT
In case of degenerative disease or lesion, bone tissue replacement and regeneration is an important clinical goal. In particular, nowadays, critical size defects rely on the engineering of scaffolds that are 3D structural supports, allowing cellular infiltration and subsequent integration with the native tissue. Several ceramic hydroxyapatite (HA) scaffolds with high porosity and good osteointegration have been developed in the past few decades but they have not solved completely the problems related to bone defects. In the present study we have developed a novel porous ceramic composite made of HA that incorporates magnetite at three different ratios: HA/Mgn 95/5, HA/Mgn 90/10 and HA/Mgn 50/50. The scaffolds, consolidated by sintering at high temperature in a controlled atmosphere, have been analysed in vitro using human osteoblast-like cells. Results indicate high biocompatibility, similar to a commercially available HA bone graft, with no negative effects arising from the presence of magnetite or by the use of a static magnetic field. HA/Mgn 90/10 was shown to enhance cell proliferation at the early stage. Moreover, it has been implanted in vivo in a critical size lesion of the rabbit condyle and a good level of histocompatibility was observed. Such results identify this scaffold as particularly relevant for bone tissue regeneration and open new perspectives for the application of a magnetic field in a clinical setting of bone replacement, either for magnetic scaffold fixation or magnetic drug delivery.

Show MeSH
Related in: MedlinePlus