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WW Domain Containing Transcription Regulator regulates human conjunctiva epithelial cell proliferation via inhibiting TGFβ signaling pathway [corrected].

Tan XW, Beuerman RW, Poh CK, Mehta JS - Mol. Vis. (2012)

Bottom Line: Immortalized conjunctiva epithelial cells (NHC) were treated with TGFβ, targeting siRNA, TGFβ receptor antibody or TGFβ receptor inhibitor, to study the involvement of TAZ and TGFβ signaling pathway in conjunctiva cell proliferation by cell adhesion assay.Moreover, TGFβ receptor antibody and TGFβ receptor inhibitor rescued this anti-proliferative effect of TAZ siRNA.TAZ is involved in human conjunctiva epithelial cells proliferation via regulating TGFβ signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Singapore Eye Research Institute, Singapore.

ABSTRACT

Purpose: To investigate the role of Tafazzin (TAZ) protein in regulating the proliferation of normal human conjunctiva epithelial cells and epithelial cells from pterygium tissue.

Methods: Conjunctiva epithelial cells were cultured in keratinocytes growth medium and treated with transformation growth factor β (TGFβ) to analyze the expression and translocation of TAZ protein by immunostaining and BrdU analysis. Immortalized conjunctiva epithelial cells (NHC) were treated with TGFβ, targeting siRNA, TGFβ receptor antibody or TGFβ receptor inhibitor, to study the involvement of TAZ and TGFβ signaling pathway in conjunctiva cell proliferation by cell adhesion assay. Conjunctiva tissues from a normal human eye and an eye with pterygium disease were collected for histological analyses and western blot to evaluate the TAZ protein expression in vivo.

Results: TAZ expression was upregulated in mitotic conjunctiva epithelial cells, proliferating conjunctiva epithelial cells, TGFβ treated conjunctiva epithelial cells and human pterygium epithelium. TAZ siRNA induced less conjunctiva epithelial cell growth. Moreover, TGFβ receptor antibody and TGFβ receptor inhibitor rescued this anti-proliferative effect of TAZ siRNA.

Conclusions: TAZ is involved in human conjunctiva epithelial cells proliferation via regulating TGFβ signaling pathway.

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Related in: MedlinePlus

Location and expression of TAZ proteins in normal or pterygium conjunctiva epithelium. A: Total tissue lysate from normal or human conjunctiva tissue with pterygium disease were blotted with anti-TAZ (A). Relative intensity of the blots was measured from three replicates (B). C: Conjunctiva tissue was stained by anti-TAZ (red) and DAPI (blue). Merged images show that the TAZ protein is expressed at the epithelial layer of conjunctiva tissue. Scale bar is 10 µm.
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f7: Location and expression of TAZ proteins in normal or pterygium conjunctiva epithelium. A: Total tissue lysate from normal or human conjunctiva tissue with pterygium disease were blotted with anti-TAZ (A). Relative intensity of the blots was measured from three replicates (B). C: Conjunctiva tissue was stained by anti-TAZ (red) and DAPI (blue). Merged images show that the TAZ protein is expressed at the epithelial layer of conjunctiva tissue. Scale bar is 10 µm.

Mentions: We then examined the expression of TAZ protein in human normal conjunctiva epithelium and pterygium conjunctiva epithelium. TAZ protein was blotted from the total lysate of the epithelial layers of conjunctiva tissues. We found that TAZ expression in pterygium tissue was much higher than that of normal tissue (Figure 7A). Intensity of the blotting bands showed that the expression level of TAZ protein in pterygium epithelium is around 10 times higher than that of the normal conjunctiva tissue (Figure 7B). Immunostaining of the pterygium conjunctiva tissue revealed that TAZ protein is highly clustering in the epithelial layer of conjunctiva tissue (Figure 7C).


WW Domain Containing Transcription Regulator regulates human conjunctiva epithelial cell proliferation via inhibiting TGFβ signaling pathway [corrected].

Tan XW, Beuerman RW, Poh CK, Mehta JS - Mol. Vis. (2012)

Location and expression of TAZ proteins in normal or pterygium conjunctiva epithelium. A: Total tissue lysate from normal or human conjunctiva tissue with pterygium disease were blotted with anti-TAZ (A). Relative intensity of the blots was measured from three replicates (B). C: Conjunctiva tissue was stained by anti-TAZ (red) and DAPI (blue). Merged images show that the TAZ protein is expressed at the epithelial layer of conjunctiva tissue. Scale bar is 10 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369895&req=5

f7: Location and expression of TAZ proteins in normal or pterygium conjunctiva epithelium. A: Total tissue lysate from normal or human conjunctiva tissue with pterygium disease were blotted with anti-TAZ (A). Relative intensity of the blots was measured from three replicates (B). C: Conjunctiva tissue was stained by anti-TAZ (red) and DAPI (blue). Merged images show that the TAZ protein is expressed at the epithelial layer of conjunctiva tissue. Scale bar is 10 µm.
Mentions: We then examined the expression of TAZ protein in human normal conjunctiva epithelium and pterygium conjunctiva epithelium. TAZ protein was blotted from the total lysate of the epithelial layers of conjunctiva tissues. We found that TAZ expression in pterygium tissue was much higher than that of normal tissue (Figure 7A). Intensity of the blotting bands showed that the expression level of TAZ protein in pterygium epithelium is around 10 times higher than that of the normal conjunctiva tissue (Figure 7B). Immunostaining of the pterygium conjunctiva tissue revealed that TAZ protein is highly clustering in the epithelial layer of conjunctiva tissue (Figure 7C).

Bottom Line: Immortalized conjunctiva epithelial cells (NHC) were treated with TGFβ, targeting siRNA, TGFβ receptor antibody or TGFβ receptor inhibitor, to study the involvement of TAZ and TGFβ signaling pathway in conjunctiva cell proliferation by cell adhesion assay.Moreover, TGFβ receptor antibody and TGFβ receptor inhibitor rescued this anti-proliferative effect of TAZ siRNA.TAZ is involved in human conjunctiva epithelial cells proliferation via regulating TGFβ signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Singapore Eye Research Institute, Singapore.

ABSTRACT

Purpose: To investigate the role of Tafazzin (TAZ) protein in regulating the proliferation of normal human conjunctiva epithelial cells and epithelial cells from pterygium tissue.

Methods: Conjunctiva epithelial cells were cultured in keratinocytes growth medium and treated with transformation growth factor β (TGFβ) to analyze the expression and translocation of TAZ protein by immunostaining and BrdU analysis. Immortalized conjunctiva epithelial cells (NHC) were treated with TGFβ, targeting siRNA, TGFβ receptor antibody or TGFβ receptor inhibitor, to study the involvement of TAZ and TGFβ signaling pathway in conjunctiva cell proliferation by cell adhesion assay. Conjunctiva tissues from a normal human eye and an eye with pterygium disease were collected for histological analyses and western blot to evaluate the TAZ protein expression in vivo.

Results: TAZ expression was upregulated in mitotic conjunctiva epithelial cells, proliferating conjunctiva epithelial cells, TGFβ treated conjunctiva epithelial cells and human pterygium epithelium. TAZ siRNA induced less conjunctiva epithelial cell growth. Moreover, TGFβ receptor antibody and TGFβ receptor inhibitor rescued this anti-proliferative effect of TAZ siRNA.

Conclusions: TAZ is involved in human conjunctiva epithelial cells proliferation via regulating TGFβ signaling pathway.

Show MeSH
Related in: MedlinePlus