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Decrease in retinal neuronal cells in streptozotocin-induced diabetic mice.

Yang Y, Mao D, Chen X, Zhao L, Tian Q, Liu C, Zhou BL - Mol. Vis. (2012)

Bottom Line: Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer.The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend.It indicates that RGC loss may be an important component of diabetic retinopathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.

ABSTRACT

Purpose: Little is known about retinal neuronal loss in the retinas of diabetic mice. The purpose of this study was the quantitative assessment of retinal neural cell number in diabetic mice.

Methods: Five-week-old C57BL/6 mice were used as a diabetic model with streptozotocin. Mice were studied over the course of 6 and 12 weeks after the onset of diabetes. Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer. The retinal ganglion cells (RGCs) were counted at two different time points after the induction of diabetes and examined using the immunofluorescence technique and quantitative analysis.

Results: The diabetic mice had significantly elevated IOP levels at 6 and 12 weeks after the onset of diabetes compared with the age-matched control mice (p<0.01 and p<0.001, respectively). The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend. At 6 and 12 weeks after the onset of diabetes, the number of Brn3a+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) and NeuN+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) was significantly lower in diabetic mice than age-matched control mice. In the retinal flatmounts, the number of Brn3a+ RGCs (p<0.05 at 6 weeks, p<0.01 at 12 weeks) was also significantly lower in diabetic mice than control mice. The IOP in diabetic mice was negatively related with RGCs in cross sections. The cut-off value of IOP was 14.2 mmHg for diabetes.

Conclusions: This is a specific quantitative study of neural cell loss in the retina during diabetes. These data suggest that retinal neural cell reduction occurs in diabetic mice. It indicates that RGC loss may be an important component of diabetic retinopathy.

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The correlation between intraocular pressure and retinal ganglion cells in diabetic mice. A: Retinal ganglion cells (RGCs) were negatively related with intraocular pressure (IOP) in cross sections (RGC-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001, respectively, n=20). B: There was no correlation between RGCs and IOP in wholemount retinas.
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f6: The correlation between intraocular pressure and retinal ganglion cells in diabetic mice. A: Retinal ganglion cells (RGCs) were negatively related with intraocular pressure (IOP) in cross sections (RGC-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001, respectively, n=20). B: There was no correlation between RGCs and IOP in wholemount retinas.

Mentions: In diabetic mice, we found increased IOP and decreased RGCs. To further investigate the relation between these results, we analyzed the correlation between IOP and RGCs. IOP was negatively correlated with RGCs in cross section (RGCs-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001; Figure 6A), and was not correlated with RGCs-Brn3a in wholemount retinas (Figure 6B).


Decrease in retinal neuronal cells in streptozotocin-induced diabetic mice.

Yang Y, Mao D, Chen X, Zhao L, Tian Q, Liu C, Zhou BL - Mol. Vis. (2012)

The correlation between intraocular pressure and retinal ganglion cells in diabetic mice. A: Retinal ganglion cells (RGCs) were negatively related with intraocular pressure (IOP) in cross sections (RGC-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001, respectively, n=20). B: There was no correlation between RGCs and IOP in wholemount retinas.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369894&req=5

f6: The correlation between intraocular pressure and retinal ganglion cells in diabetic mice. A: Retinal ganglion cells (RGCs) were negatively related with intraocular pressure (IOP) in cross sections (RGC-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001, respectively, n=20). B: There was no correlation between RGCs and IOP in wholemount retinas.
Mentions: In diabetic mice, we found increased IOP and decreased RGCs. To further investigate the relation between these results, we analyzed the correlation between IOP and RGCs. IOP was negatively correlated with RGCs in cross section (RGCs-Brn3a: r=-0.589, p<0.01; RGCs-NeuN: r=-0.789, p<0.001; Figure 6A), and was not correlated with RGCs-Brn3a in wholemount retinas (Figure 6B).

Bottom Line: Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer.The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend.It indicates that RGC loss may be an important component of diabetic retinopathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.

ABSTRACT

Purpose: Little is known about retinal neuronal loss in the retinas of diabetic mice. The purpose of this study was the quantitative assessment of retinal neural cell number in diabetic mice.

Methods: Five-week-old C57BL/6 mice were used as a diabetic model with streptozotocin. Mice were studied over the course of 6 and 12 weeks after the onset of diabetes. Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer. The retinal ganglion cells (RGCs) were counted at two different time points after the induction of diabetes and examined using the immunofluorescence technique and quantitative analysis.

Results: The diabetic mice had significantly elevated IOP levels at 6 and 12 weeks after the onset of diabetes compared with the age-matched control mice (p<0.01 and p<0.001, respectively). The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend. At 6 and 12 weeks after the onset of diabetes, the number of Brn3a+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) and NeuN+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) was significantly lower in diabetic mice than age-matched control mice. In the retinal flatmounts, the number of Brn3a+ RGCs (p<0.05 at 6 weeks, p<0.01 at 12 weeks) was also significantly lower in diabetic mice than control mice. The IOP in diabetic mice was negatively related with RGCs in cross sections. The cut-off value of IOP was 14.2 mmHg for diabetes.

Conclusions: This is a specific quantitative study of neural cell loss in the retina during diabetes. These data suggest that retinal neural cell reduction occurs in diabetic mice. It indicates that RGC loss may be an important component of diabetic retinopathy.

Show MeSH
Related in: MedlinePlus