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Decrease in retinal neuronal cells in streptozotocin-induced diabetic mice.

Yang Y, Mao D, Chen X, Zhao L, Tian Q, Liu C, Zhou BL - Mol. Vis. (2012)

Bottom Line: Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer.The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend.It indicates that RGC loss may be an important component of diabetic retinopathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.

ABSTRACT

Purpose: Little is known about retinal neuronal loss in the retinas of diabetic mice. The purpose of this study was the quantitative assessment of retinal neural cell number in diabetic mice.

Methods: Five-week-old C57BL/6 mice were used as a diabetic model with streptozotocin. Mice were studied over the course of 6 and 12 weeks after the onset of diabetes. Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer. The retinal ganglion cells (RGCs) were counted at two different time points after the induction of diabetes and examined using the immunofluorescence technique and quantitative analysis.

Results: The diabetic mice had significantly elevated IOP levels at 6 and 12 weeks after the onset of diabetes compared with the age-matched control mice (p<0.01 and p<0.001, respectively). The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend. At 6 and 12 weeks after the onset of diabetes, the number of Brn3a+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) and NeuN+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) was significantly lower in diabetic mice than age-matched control mice. In the retinal flatmounts, the number of Brn3a+ RGCs (p<0.05 at 6 weeks, p<0.01 at 12 weeks) was also significantly lower in diabetic mice than control mice. The IOP in diabetic mice was negatively related with RGCs in cross sections. The cut-off value of IOP was 14.2 mmHg for diabetes.

Conclusions: This is a specific quantitative study of neural cell loss in the retina during diabetes. These data suggest that retinal neural cell reduction occurs in diabetic mice. It indicates that RGC loss may be an important component of diabetic retinopathy.

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Related in: MedlinePlus

Intraocular pressure in streptozotocin-induced diabetic and control mice. The 6- and 12-week diabetic mice had a significantly higher intraocular pressure (IOP) than the age-matched control mice. Compared with 6-week diabetic mice, 12-week diabetic mice also had significantly increased IOP (n=8/group, ** indicates p<0.01, *** indicates p<0.001).
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f1: Intraocular pressure in streptozotocin-induced diabetic and control mice. The 6- and 12-week diabetic mice had a significantly higher intraocular pressure (IOP) than the age-matched control mice. Compared with 6-week diabetic mice, 12-week diabetic mice also had significantly increased IOP (n=8/group, ** indicates p<0.01, *** indicates p<0.001).

Mentions: Loss of RGCs occurs in many ophthalmic conditions such as glaucoma [20] and diabetes [30]. Some of the molecular and cellular mechanisms that may be involved in RGC loss in ocular conditions are associated with elevated IOP. Therefore, in this study, we have compared the IOP of diabetic mice with that of age-matched controls (Figure 1). The levels of IOP in 6- and 12-week diabetic mice was significantly higher than those of age-matched control mice (11.94±1.53 mmHg versus 14.78±1.20 mmHg, p<0.01; 12.02±1.33 mmHg versus 19.52±1.89 mmHg, p<0.001). On the other hand, there was also a significantly elevated IOP comparing 6-week diabetic mice with 12-week diabetic mice (14.78±1.20 mmHg versus 19.52±1.89 mmHg, p<0.01).


Decrease in retinal neuronal cells in streptozotocin-induced diabetic mice.

Yang Y, Mao D, Chen X, Zhao L, Tian Q, Liu C, Zhou BL - Mol. Vis. (2012)

Intraocular pressure in streptozotocin-induced diabetic and control mice. The 6- and 12-week diabetic mice had a significantly higher intraocular pressure (IOP) than the age-matched control mice. Compared with 6-week diabetic mice, 12-week diabetic mice also had significantly increased IOP (n=8/group, ** indicates p<0.01, *** indicates p<0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369894&req=5

f1: Intraocular pressure in streptozotocin-induced diabetic and control mice. The 6- and 12-week diabetic mice had a significantly higher intraocular pressure (IOP) than the age-matched control mice. Compared with 6-week diabetic mice, 12-week diabetic mice also had significantly increased IOP (n=8/group, ** indicates p<0.01, *** indicates p<0.001).
Mentions: Loss of RGCs occurs in many ophthalmic conditions such as glaucoma [20] and diabetes [30]. Some of the molecular and cellular mechanisms that may be involved in RGC loss in ocular conditions are associated with elevated IOP. Therefore, in this study, we have compared the IOP of diabetic mice with that of age-matched controls (Figure 1). The levels of IOP in 6- and 12-week diabetic mice was significantly higher than those of age-matched control mice (11.94±1.53 mmHg versus 14.78±1.20 mmHg, p<0.01; 12.02±1.33 mmHg versus 19.52±1.89 mmHg, p<0.001). On the other hand, there was also a significantly elevated IOP comparing 6-week diabetic mice with 12-week diabetic mice (14.78±1.20 mmHg versus 19.52±1.89 mmHg, p<0.01).

Bottom Line: Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer.The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend.It indicates that RGC loss may be an important component of diabetic retinopathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing, China.

ABSTRACT

Purpose: Little is known about retinal neuronal loss in the retinas of diabetic mice. The purpose of this study was the quantitative assessment of retinal neural cell number in diabetic mice.

Methods: Five-week-old C57BL/6 mice were used as a diabetic model with streptozotocin. Mice were studied over the course of 6 and 12 weeks after the onset of diabetes. Intraocular pressure (IOP) was measured with a noninvasive TonoLab tonometer. The retinal ganglion cells (RGCs) were counted at two different time points after the induction of diabetes and examined using the immunofluorescence technique and quantitative analysis.

Results: The diabetic mice had significantly elevated IOP levels at 6 and 12 weeks after the onset of diabetes compared with the age-matched control mice (p<0.01 and p<0.001, respectively). The temporal course of Brn3a+ RGC and Neuronal Nuclei+RGC (NeuN+ RGC) loss induced by intraperitoneal injection of streptozotocin followed a similar trend. At 6 and 12 weeks after the onset of diabetes, the number of Brn3a+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) and NeuN+ RGCs (p<0.05 at 6 weeks; p<0.001 at 12 weeks) was significantly lower in diabetic mice than age-matched control mice. In the retinal flatmounts, the number of Brn3a+ RGCs (p<0.05 at 6 weeks, p<0.01 at 12 weeks) was also significantly lower in diabetic mice than control mice. The IOP in diabetic mice was negatively related with RGCs in cross sections. The cut-off value of IOP was 14.2 mmHg for diabetes.

Conclusions: This is a specific quantitative study of neural cell loss in the retina during diabetes. These data suggest that retinal neural cell reduction occurs in diabetic mice. It indicates that RGC loss may be an important component of diabetic retinopathy.

Show MeSH
Related in: MedlinePlus