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Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

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Related in: MedlinePlus

Expression of selected genes in rat retina as measured by quantitative reverse transcriptase PCR (qRT–PCR). Retinas were harvested from rats maintained under cyclic dim or bright light exposure for eight weeks. Expression values were normalized with the housekeeping gene, Rpl19. Expression values (±SD) are presented as fold change over the CONT+DL value, which was set to 1.0 (n=4 samples). CONT+DL: control diet under cyclic dim light; caffeic acid phenethyl ester (CAPE)+DL: CAPE diet under cyclic dim light; CONT+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
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f9: Expression of selected genes in rat retina as measured by quantitative reverse transcriptase PCR (qRT–PCR). Retinas were harvested from rats maintained under cyclic dim or bright light exposure for eight weeks. Expression values were normalized with the housekeeping gene, Rpl19. Expression values (±SD) are presented as fold change over the CONT+DL value, which was set to 1.0 (n=4 samples). CONT+DL: control diet under cyclic dim light; caffeic acid phenethyl ester (CAPE)+DL: CAPE diet under cyclic dim light; CONT+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).

Mentions: Compared to the retinas from rats fed with the control diet in DL, the retinas from the CAPE-fed rats in DL had significantly higher expression levels of Ho1 and Icam1 (p<0.05) and significantly lower expression levels of Fosl1 (proapoptotic) and Lox12 (proinflammatory) (2.5-fold and one-fold, respectively, p<0.05 for each, Figure 9). However, no significant differences were found in the expression of Cox2, catalase, Cxcl1, Lox5, Ccl2, IL6, C-fos, Thx1, Nfkb, and Gfap.


Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Expression of selected genes in rat retina as measured by quantitative reverse transcriptase PCR (qRT–PCR). Retinas were harvested from rats maintained under cyclic dim or bright light exposure for eight weeks. Expression values were normalized with the housekeeping gene, Rpl19. Expression values (±SD) are presented as fold change over the CONT+DL value, which was set to 1.0 (n=4 samples). CONT+DL: control diet under cyclic dim light; caffeic acid phenethyl ester (CAPE)+DL: CAPE diet under cyclic dim light; CONT+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369890&req=5

f9: Expression of selected genes in rat retina as measured by quantitative reverse transcriptase PCR (qRT–PCR). Retinas were harvested from rats maintained under cyclic dim or bright light exposure for eight weeks. Expression values were normalized with the housekeeping gene, Rpl19. Expression values (±SD) are presented as fold change over the CONT+DL value, which was set to 1.0 (n=4 samples). CONT+DL: control diet under cyclic dim light; caffeic acid phenethyl ester (CAPE)+DL: CAPE diet under cyclic dim light; CONT+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
Mentions: Compared to the retinas from rats fed with the control diet in DL, the retinas from the CAPE-fed rats in DL had significantly higher expression levels of Ho1 and Icam1 (p<0.05) and significantly lower expression levels of Fosl1 (proapoptotic) and Lox12 (proinflammatory) (2.5-fold and one-fold, respectively, p<0.05 for each, Figure 9). However, no significant differences were found in the expression of Cox2, catalase, Cxcl1, Lox5, Ccl2, IL6, C-fos, Thx1, Nfkb, and Gfap.

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

Show MeSH
Related in: MedlinePlus