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Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

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Related in: MedlinePlus

Cone photoreceptor function was measured by electroretinography (ERG) after eight weeks of feeding with control and caffeic acid phenethyl ester (CAPE) diet. Cone photoreceptor function was measured with single-flash photopic ERG with a flash stimulus of 3.7 log cd.s/m2 that was presented to dilated, light-adapted (5 min at 2.0 log cd.s/m2) rats. There is no significant difference (n=12/group) observed in cone function among the rats fed with CAPE and reared in different light conditions. Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light.
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f7: Cone photoreceptor function was measured by electroretinography (ERG) after eight weeks of feeding with control and caffeic acid phenethyl ester (CAPE) diet. Cone photoreceptor function was measured with single-flash photopic ERG with a flash stimulus of 3.7 log cd.s/m2 that was presented to dilated, light-adapted (5 min at 2.0 log cd.s/m2) rats. There is no significant difference (n=12/group) observed in cone function among the rats fed with CAPE and reared in different light conditions. Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light.

Mentions: To further confirm the enhanced ERG responses from rats fed with CAPE in cyclic dim light, the rat number was increased from eight to 12 in each group, and the duration of the feeding period was increased from three weeks to eight weeks in cyclic dim light (50 lux). Another group that was fed for eight weeks with CAPE and exposed to bright cyclic light (200 lux) was established to determine whether CAPE can protect the retina from cyclic-bright-light-induced, chronic photoreceptor loss. Similar to the three-week feeding results (Figure 4), the eight-week CAPE feeding resulted in significantly higher ERG responses in the dim-reared rats (scotopic A and B; p<0.01; Figure 6A). However, no significant difference was observed in the ERG responses between the CAPE- and control-fed rats maintained in bright cyclic light (200 lux) for eight weeks (Figure 6B). The cone response as measured with photopic ERG, and the ONL thicknesses were still not significantly different between the CAPE- and the control-fed rats under cyclic dim or bright light (Figure 7 and Figure 8). The retinas were further examined using biochemical and molecular analyses to understand the effect of dietary CAPE on retinal tissue, with a focus on oxidative and inflammatory markers.


Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Cone photoreceptor function was measured by electroretinography (ERG) after eight weeks of feeding with control and caffeic acid phenethyl ester (CAPE) diet. Cone photoreceptor function was measured with single-flash photopic ERG with a flash stimulus of 3.7 log cd.s/m2 that was presented to dilated, light-adapted (5 min at 2.0 log cd.s/m2) rats. There is no significant difference (n=12/group) observed in cone function among the rats fed with CAPE and reared in different light conditions. Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369890&req=5

f7: Cone photoreceptor function was measured by electroretinography (ERG) after eight weeks of feeding with control and caffeic acid phenethyl ester (CAPE) diet. Cone photoreceptor function was measured with single-flash photopic ERG with a flash stimulus of 3.7 log cd.s/m2 that was presented to dilated, light-adapted (5 min at 2.0 log cd.s/m2) rats. There is no significant difference (n=12/group) observed in cone function among the rats fed with CAPE and reared in different light conditions. Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light.
Mentions: To further confirm the enhanced ERG responses from rats fed with CAPE in cyclic dim light, the rat number was increased from eight to 12 in each group, and the duration of the feeding period was increased from three weeks to eight weeks in cyclic dim light (50 lux). Another group that was fed for eight weeks with CAPE and exposed to bright cyclic light (200 lux) was established to determine whether CAPE can protect the retina from cyclic-bright-light-induced, chronic photoreceptor loss. Similar to the three-week feeding results (Figure 4), the eight-week CAPE feeding resulted in significantly higher ERG responses in the dim-reared rats (scotopic A and B; p<0.01; Figure 6A). However, no significant difference was observed in the ERG responses between the CAPE- and control-fed rats maintained in bright cyclic light (200 lux) for eight weeks (Figure 6B). The cone response as measured with photopic ERG, and the ONL thicknesses were still not significantly different between the CAPE- and the control-fed rats under cyclic dim or bright light (Figure 7 and Figure 8). The retinas were further examined using biochemical and molecular analyses to understand the effect of dietary CAPE on retinal tissue, with a focus on oxidative and inflammatory markers.

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

Show MeSH
Related in: MedlinePlus