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Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

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Related in: MedlinePlus

Retina outer nuclear layer (ONL) thickness from rats fed for three weeks under cyclic dim light. The ONL thickness measured in μM at each defined area from superior to inferior through optic nerve. There is no significant difference in ONL thickness of caffeic acid phenethyl ester (CAPE)-fed retina and control-fed retina (p>0.05, with the Student t test; n=8). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light.
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f5: Retina outer nuclear layer (ONL) thickness from rats fed for three weeks under cyclic dim light. The ONL thickness measured in μM at each defined area from superior to inferior through optic nerve. There is no significant difference in ONL thickness of caffeic acid phenethyl ester (CAPE)-fed retina and control-fed retina (p>0.05, with the Student t test; n=8). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light.

Mentions: To understand CAPE’s role in the retina in vivo, the SD rats’ diet was supplemented with CAPE, and then the rats were subjected to either acute intense light stress or chronic cyclic dim/bright light exposure and their retinas analyzed from structural, functional, and biochemical standpoints. Feeding rats with a 0.02% CAPE diet for two weeks did not protect the retinas from intense-light-induced damage (2,700 lux for 6 h, data not shown). Interestingly, compared to the rats fed with the control diet, the CAPE-fed rats maintained in dim light (DL; 50 lux for 3 weeks, 7 AM to 7 PM) had significantly higher ERG scotopic A and B wave amplitudes (Figure 4); however, no significant difference was observed in the photopic ERG (data not shown) or in the photoreceptor cell numbers (measured by ONL thickness; Figure 5).


Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Retina outer nuclear layer (ONL) thickness from rats fed for three weeks under cyclic dim light. The ONL thickness measured in μM at each defined area from superior to inferior through optic nerve. There is no significant difference in ONL thickness of caffeic acid phenethyl ester (CAPE)-fed retina and control-fed retina (p>0.05, with the Student t test; n=8). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369890&req=5

f5: Retina outer nuclear layer (ONL) thickness from rats fed for three weeks under cyclic dim light. The ONL thickness measured in μM at each defined area from superior to inferior through optic nerve. There is no significant difference in ONL thickness of caffeic acid phenethyl ester (CAPE)-fed retina and control-fed retina (p>0.05, with the Student t test; n=8). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light.
Mentions: To understand CAPE’s role in the retina in vivo, the SD rats’ diet was supplemented with CAPE, and then the rats were subjected to either acute intense light stress or chronic cyclic dim/bright light exposure and their retinas analyzed from structural, functional, and biochemical standpoints. Feeding rats with a 0.02% CAPE diet for two weeks did not protect the retinas from intense-light-induced damage (2,700 lux for 6 h, data not shown). Interestingly, compared to the rats fed with the control diet, the CAPE-fed rats maintained in dim light (DL; 50 lux for 3 weeks, 7 AM to 7 PM) had significantly higher ERG scotopic A and B wave amplitudes (Figure 4); however, no significant difference was observed in the photopic ERG (data not shown) or in the photoreceptor cell numbers (measured by ONL thickness; Figure 5).

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

Show MeSH
Related in: MedlinePlus