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Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

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Related in: MedlinePlus

Expression and quantification of selected proteins in the retina from rats fed for eight weeks with control and caffeic acid phenethyl ester (CAPE) diet. Retinas were harvested from CAPE- and control diet-fed rats maintained under cyclic dim or bright light exposure for eight weeks. A: Retinal whole cell proteins were extracted and subjected to western blotting with anti-LOX5, and anti-COX-2 antibodies, and the cytoplasmic and nuclear fraction separately with anti-NFκB antibodies. B: Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei and NFκB (c) in cytosol from the retinas was obtained with densitometric analysis, and normalized by β-actin, (n=4). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
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f10: Expression and quantification of selected proteins in the retina from rats fed for eight weeks with control and caffeic acid phenethyl ester (CAPE) diet. Retinas were harvested from CAPE- and control diet-fed rats maintained under cyclic dim or bright light exposure for eight weeks. A: Retinal whole cell proteins were extracted and subjected to western blotting with anti-LOX5, and anti-COX-2 antibodies, and the cytoplasmic and nuclear fraction separately with anti-NFκB antibodies. B: Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei and NFκB (c) in cytosol from the retinas was obtained with densitometric analysis, and normalized by β-actin, (n=4). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).

Mentions: In addition, the NFκB protein level in the nuclear fraction was significantly lower in the retinas from CAPE-fed rats under cyclic dim light than in the retinas from rats fed the control diet, but the protein levels of LOX5, COX-2, and cytosolic NFκB were not different between the two groups (Figure 10A,B).


Caffeic acid phenethyl ester protects 661W cells from H2O2-mediated cell death and enhances electroretinography response in dim-reared albino rats.

Chen H, Tran JT, Anderson RE, Mandal MN - Mol. Vis. (2012)

Expression and quantification of selected proteins in the retina from rats fed for eight weeks with control and caffeic acid phenethyl ester (CAPE) diet. Retinas were harvested from CAPE- and control diet-fed rats maintained under cyclic dim or bright light exposure for eight weeks. A: Retinal whole cell proteins were extracted and subjected to western blotting with anti-LOX5, and anti-COX-2 antibodies, and the cytoplasmic and nuclear fraction separately with anti-NFκB antibodies. B: Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei and NFκB (c) in cytosol from the retinas was obtained with densitometric analysis, and normalized by β-actin, (n=4). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369890&req=5

f10: Expression and quantification of selected proteins in the retina from rats fed for eight weeks with control and caffeic acid phenethyl ester (CAPE) diet. Retinas were harvested from CAPE- and control diet-fed rats maintained under cyclic dim or bright light exposure for eight weeks. A: Retinal whole cell proteins were extracted and subjected to western blotting with anti-LOX5, and anti-COX-2 antibodies, and the cytoplasmic and nuclear fraction separately with anti-NFκB antibodies. B: Quantification of LOX5, COX-2, and NFκB (n) expression in nuclei and NFκB (c) in cytosol from the retinas was obtained with densitometric analysis, and normalized by β-actin, (n=4). Cont+DL: control diet under cyclic dim light; CAPE+DL: CAPE diet under cyclic dim light; Cont+BL: control diet under cyclic bright light; CAPE+BL: CAPE diet under cyclic bright light. (*: p<0.05, with the Student t test).
Mentions: In addition, the NFκB protein level in the nuclear fraction was significantly lower in the retinas from CAPE-fed rats under cyclic dim light than in the retinas from rats fed the control diet, but the protein levels of LOX5, COX-2, and cytosolic NFκB were not different between the two groups (Figure 10A,B).

Bottom Line: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties.Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1).CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

ABSTRACT

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H(2)O(2)-mediated cell death and in albino rats (in vivo) against various light conditions.

Methods: The 661W cells were pretreated with CAPE and then stressed with H(2)O(2). Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding.

Results: Pretreatment of 661W cells with CAPE reduced H(2)O(2)-mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light.

Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

Show MeSH
Related in: MedlinePlus