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Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

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Related in: MedlinePlus

Principal component analysis (PCA) of 15 metabolites in nodules from the plants subjected to water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean nodule samples. NTC: Nodule Tolerant Control, NTD: Nodule Tolerant Drought, NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought.
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pone-0038554-g005: Principal component analysis (PCA) of 15 metabolites in nodules from the plants subjected to water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean nodule samples. NTC: Nodule Tolerant Control, NTD: Nodule Tolerant Drought, NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought.

Mentions: In nodules, the PCA analysis (Figure 5a) showed that the first two PCs represented 48.2% of the initial variability contained in the original data. The scores plot exhibited separation between all four groups (NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought, NTC: Nodule Tolerant Control and NTD: Nodule Tolerant Drought) when PC2 and PC1 were used as variables. It seems that with a few exceptions, the samples of tolerant plants are separated from sensitive ones along PC1, while control samples are separated from stressed ones along PC2. Plot of loadings (Figure 5b) show the variables responsible for this separation. The metabolites sucrose, aspartate, glutamine, GABA, allantoin, and succinic acid play a crucial role in the separation of tolerant from sensitive samples. On the other hand, the separation of controls from the stressed samples is due to the variations in the levels of malic acid, 2-oxoglutaric acid, fumaric acid, and sucrose.


Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Principal component analysis (PCA) of 15 metabolites in nodules from the plants subjected to water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean nodule samples. NTC: Nodule Tolerant Control, NTD: Nodule Tolerant Drought, NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369847&req=5

pone-0038554-g005: Principal component analysis (PCA) of 15 metabolites in nodules from the plants subjected to water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean nodule samples. NTC: Nodule Tolerant Control, NTD: Nodule Tolerant Drought, NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought.
Mentions: In nodules, the PCA analysis (Figure 5a) showed that the first two PCs represented 48.2% of the initial variability contained in the original data. The scores plot exhibited separation between all four groups (NSC: Nodule Sensitive Control, NSD: Nodule Sensitive Drought, NTC: Nodule Tolerant Control and NTD: Nodule Tolerant Drought) when PC2 and PC1 were used as variables. It seems that with a few exceptions, the samples of tolerant plants are separated from sensitive ones along PC1, while control samples are separated from stressed ones along PC2. Plot of loadings (Figure 5b) show the variables responsible for this separation. The metabolites sucrose, aspartate, glutamine, GABA, allantoin, and succinic acid play a crucial role in the separation of tolerant from sensitive samples. On the other hand, the separation of controls from the stressed samples is due to the variations in the levels of malic acid, 2-oxoglutaric acid, fumaric acid, and sucrose.

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

Show MeSH
Related in: MedlinePlus