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Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

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Principal component analysis (PCA) of 15 metabolites in the leaves from the plants grown under water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean leaf samples. LTC: Leaf Tolerant Control, LTD: Leaf Tolerant Drought, LSC: Leaf Sensitive Control, LSD: Leaf Sensitive Drought.
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pone-0038554-g004: Principal component analysis (PCA) of 15 metabolites in the leaves from the plants grown under water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean leaf samples. LTC: Leaf Tolerant Control, LTD: Leaf Tolerant Drought, LSC: Leaf Sensitive Control, LSD: Leaf Sensitive Drought.

Mentions: A scores scatter plot of the first two PCs obtained considering all 1H NMR data derived from the leaves shows a good separation of all four groups (LTC: leaf tolerant control, LTD: leaf tolerant drought, LSC: leaf sensitive control and LSD: leaf sensitive drought) along PC1 axis (Figure 4a). It seems that this separation is due to the treatment (control vs stressed) with further separation between stressed sensitive and stressed tolerant genotypes. The greatest separation along PC1 is between LSC and LSD groups whereas the separation between LTC and LTD is less apparent along PC1 axis, but noticeable along PC2 (see arrows on Figure 4a). This behaviour of the data evidences a markedly more profound metabolic impact of drought stress on sensitive plants with respect to tolerant ones. The separation between leaf samples of well watered and stressed plants along PC1 axis seemed to be mainly attributable to aspartate, succinic acid, sucrose, malic acid, alanine, GABA, myo-inositol and 2-oxoglutaric acid as shown in the complementary PCA loading plot (Figure 4b). In case of the tolerant genotype, leaf samples of control and stressed plants are well separated along PC2 due to the metabolites pinitol, citric acid, choline, and allantoin.


Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Principal component analysis (PCA) of 15 metabolites in the leaves from the plants grown under water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean leaf samples. LTC: Leaf Tolerant Control, LTD: Leaf Tolerant Drought, LSC: Leaf Sensitive Control, LSD: Leaf Sensitive Drought.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369847&req=5

pone-0038554-g004: Principal component analysis (PCA) of 15 metabolites in the leaves from the plants grown under water sufficient and deficient conditions.Score (a) and loading plot (b) of soybean leaf samples. LTC: Leaf Tolerant Control, LTD: Leaf Tolerant Drought, LSC: Leaf Sensitive Control, LSD: Leaf Sensitive Drought.
Mentions: A scores scatter plot of the first two PCs obtained considering all 1H NMR data derived from the leaves shows a good separation of all four groups (LTC: leaf tolerant control, LTD: leaf tolerant drought, LSC: leaf sensitive control and LSD: leaf sensitive drought) along PC1 axis (Figure 4a). It seems that this separation is due to the treatment (control vs stressed) with further separation between stressed sensitive and stressed tolerant genotypes. The greatest separation along PC1 is between LSC and LSD groups whereas the separation between LTC and LTD is less apparent along PC1 axis, but noticeable along PC2 (see arrows on Figure 4a). This behaviour of the data evidences a markedly more profound metabolic impact of drought stress on sensitive plants with respect to tolerant ones. The separation between leaf samples of well watered and stressed plants along PC1 axis seemed to be mainly attributable to aspartate, succinic acid, sucrose, malic acid, alanine, GABA, myo-inositol and 2-oxoglutaric acid as shown in the complementary PCA loading plot (Figure 4b). In case of the tolerant genotype, leaf samples of control and stressed plants are well separated along PC2 due to the metabolites pinitol, citric acid, choline, and allantoin.

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

Show MeSH
Related in: MedlinePlus