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Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

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Schematic representation of the selected metabolic pathways affected by drought in two soybean genotypes contrasting in sensitivity/tolerance to water stress.Histograms represent relative changes in the level of the metabolites (arbitrary units) in trifoliate leaves and nodules in the plants subjected to water stress. Values are presented as the mean ± standard deviation (SD) of nine independent biological determinations. TC: Tolerant control; TD: Tolerant Drought, SC: Sensitive Control; SD: Sensitive Drought.
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pone-0038554-g003: Schematic representation of the selected metabolic pathways affected by drought in two soybean genotypes contrasting in sensitivity/tolerance to water stress.Histograms represent relative changes in the level of the metabolites (arbitrary units) in trifoliate leaves and nodules in the plants subjected to water stress. Values are presented as the mean ± standard deviation (SD) of nine independent biological determinations. TC: Tolerant control; TD: Tolerant Drought, SC: Sensitive Control; SD: Sensitive Drought.

Mentions: Basically, regardless of the genotype (tolerant or sensitive plants) or watering conditions, all the 1H NMR spectra of extracts from the same type of tissues (leaves or nodules) share the same signals, although their relative intensity is variable. The intensity of selected signals (Table 1 and Materials and Methods section) was used to calculate the relative molecular abundance of about 15 assigned metabolites. On the other hand, the assignment of minor components was hindered by the scarcity of data on these metabolic compounds in literature. Although the number of compounds identified by NMR is limited, the NMR spectra indeed gave a good picture of what really is present in the plant extracts examined. Results of the study demonstrated that water stress induces several changes in various metabolic pathways in both genotypes; the effect being more pronounced in the leaves than in nodules (Figure 3 and Tables 2,3,4).


Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Schematic representation of the selected metabolic pathways affected by drought in two soybean genotypes contrasting in sensitivity/tolerance to water stress.Histograms represent relative changes in the level of the metabolites (arbitrary units) in trifoliate leaves and nodules in the plants subjected to water stress. Values are presented as the mean ± standard deviation (SD) of nine independent biological determinations. TC: Tolerant control; TD: Tolerant Drought, SC: Sensitive Control; SD: Sensitive Drought.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369847&req=5

pone-0038554-g003: Schematic representation of the selected metabolic pathways affected by drought in two soybean genotypes contrasting in sensitivity/tolerance to water stress.Histograms represent relative changes in the level of the metabolites (arbitrary units) in trifoliate leaves and nodules in the plants subjected to water stress. Values are presented as the mean ± standard deviation (SD) of nine independent biological determinations. TC: Tolerant control; TD: Tolerant Drought, SC: Sensitive Control; SD: Sensitive Drought.
Mentions: Basically, regardless of the genotype (tolerant or sensitive plants) or watering conditions, all the 1H NMR spectra of extracts from the same type of tissues (leaves or nodules) share the same signals, although their relative intensity is variable. The intensity of selected signals (Table 1 and Materials and Methods section) was used to calculate the relative molecular abundance of about 15 assigned metabolites. On the other hand, the assignment of minor components was hindered by the scarcity of data on these metabolic compounds in literature. Although the number of compounds identified by NMR is limited, the NMR spectra indeed gave a good picture of what really is present in the plant extracts examined. Results of the study demonstrated that water stress induces several changes in various metabolic pathways in both genotypes; the effect being more pronounced in the leaves than in nodules (Figure 3 and Tables 2,3,4).

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

Show MeSH
Related in: MedlinePlus