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Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

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1H NMR spectra of (a) leaves and (b) nodules of water-soluble extracts from well-watered tolerant soybean plants.Assignments: 1, alanine; 2, GABA; 3, glutamine; 4, malic acid; 5, succinic acid; 6, citric acid; 7, aspartate; 8, asparagine; 9, myo-inositol; 10, choline; 11, pinitol; 12, sucrose; 13, fumaric acid; 14, trigonelline. HDO: deuterated water.
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pone-0038554-g002: 1H NMR spectra of (a) leaves and (b) nodules of water-soluble extracts from well-watered tolerant soybean plants.Assignments: 1, alanine; 2, GABA; 3, glutamine; 4, malic acid; 5, succinic acid; 6, citric acid; 7, aspartate; 8, asparagine; 9, myo-inositol; 10, choline; 11, pinitol; 12, sucrose; 13, fumaric acid; 14, trigonelline. HDO: deuterated water.

Mentions: Comparison of the 1H NMR spectra revealed no major qualitative differences in the metabolites between leaf and nodule tissues except for minor aromatic compounds (Figure 2 and Table 1). As an example, a typical 1H NMR spectra of leaf and nodule extracts of well watered tolerant soybean plants together with the assignment of the most abundant metabolites (amino acids, sugars, organic acids) are shown in the Figures 2a and 2b, respectively. Among the resonances in the 9.5 – 7 ppm region, characteristic for aromatic and heteroaromatic compounds, only trigonelline was assigned in leaves extracts. On the other hand, asparagine was detected exclusively in nodules.


Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

1H NMR spectra of (a) leaves and (b) nodules of water-soluble extracts from well-watered tolerant soybean plants.Assignments: 1, alanine; 2, GABA; 3, glutamine; 4, malic acid; 5, succinic acid; 6, citric acid; 7, aspartate; 8, asparagine; 9, myo-inositol; 10, choline; 11, pinitol; 12, sucrose; 13, fumaric acid; 14, trigonelline. HDO: deuterated water.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369847&req=5

pone-0038554-g002: 1H NMR spectra of (a) leaves and (b) nodules of water-soluble extracts from well-watered tolerant soybean plants.Assignments: 1, alanine; 2, GABA; 3, glutamine; 4, malic acid; 5, succinic acid; 6, citric acid; 7, aspartate; 8, asparagine; 9, myo-inositol; 10, choline; 11, pinitol; 12, sucrose; 13, fumaric acid; 14, trigonelline. HDO: deuterated water.
Mentions: Comparison of the 1H NMR spectra revealed no major qualitative differences in the metabolites between leaf and nodule tissues except for minor aromatic compounds (Figure 2 and Table 1). As an example, a typical 1H NMR spectra of leaf and nodule extracts of well watered tolerant soybean plants together with the assignment of the most abundant metabolites (amino acids, sugars, organic acids) are shown in the Figures 2a and 2b, respectively. Among the resonances in the 9.5 – 7 ppm region, characteristic for aromatic and heteroaromatic compounds, only trigonelline was assigned in leaves extracts. On the other hand, asparagine was detected exclusively in nodules.

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

Show MeSH
Related in: MedlinePlus