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Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

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Responses of drought tolerant and sensitive varieties of soybean towards short-term water stress.(a–d) trifoliate leaves and (e–h) nodules. (a, e) dry weight (DW); (b) relative water content (RWC), (f) nitrogenase activity, (c, g) ureide levels and (d, h) proline levels in 17-day-old plants subjected to 10 days water sufficient and deficient conditions. TC: Tolerant genotype Control; TD: Tolerant subjected to Drought, SC: Sensitive genotype Control; SD: Sensitive subjected to Drought. Data represent the mean ± standard deviation (SD) of five to 15 replicates. Different letters at the top of each bar indicate significant differences at P<0.01.
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pone-0038554-g001: Responses of drought tolerant and sensitive varieties of soybean towards short-term water stress.(a–d) trifoliate leaves and (e–h) nodules. (a, e) dry weight (DW); (b) relative water content (RWC), (f) nitrogenase activity, (c, g) ureide levels and (d, h) proline levels in 17-day-old plants subjected to 10 days water sufficient and deficient conditions. TC: Tolerant genotype Control; TD: Tolerant subjected to Drought, SC: Sensitive genotype Control; SD: Sensitive subjected to Drought. Data represent the mean ± standard deviation (SD) of five to 15 replicates. Different letters at the top of each bar indicate significant differences at P<0.01.

Mentions: The two soybean cultivars used in the present study were categorized at CIAP-INTA (Centro de Investigaciones Agropecuarias – Instituto Nacional de Tecnología Agropecuaria, Argentina) as tolerant (NA5009RG) and sensitive (DM50048) to drought stress based on their ability to maintain relative water content (RWC) and growth, and withstand oxidative stress through the modulation of cellular malondialdehide (MDA) levels. The drought tolerant line maintained higher RWC and showed greater ability to withstand oxidative damage owing to lower production of MDA, and exhibited sustained growth at reduced soil moisture conditions (Dr. Celina Luna, personal communication). In the present study, a comparison of these two soybean drought tolerant and sensitive genotypes was undertaken to determine the differences in their metabolic profiles/responses during water stress. Drought was imposed on nodulated soybean plants 17 days after inoculation by withholding water during 10 days, and physiological characteristics such as dry weight (DW), relative water content (RWC), chlorophyll and nitrogenase activity (ARA), and metabolite profiles were analyzed in order to establish the effect of water stress on these plant parameters (Figure 1). The results of the study showed that the water stress produced varied effects on leaf and nodule metabolism in both drought tolerant and sensitive soybean varieties. Under the drought condition imposed, both RWC and DW of the leaves showed a reduction, more remarkably in the sensitive variety (a decrease of about 10% in RWC and 42% in DW in the sensitive genotype as compared to 9% and 15%, respectively, in the tolerant one; Figure 1a, b). In addition, water stress affected proline and ureide contents in leaves of the sensitive variety but not in the tolerant genotype (Figure 1c, d). On the other hand, no effect of drought stress was observed on chlorophyll content as well as chlorophyll a/b ratio in both the genotypes (data not shown).


Metabolite adjustments in drought tolerant and sensitive soybean genotypes in response to water stress.

Silvente S, Sobolev AP, Lara M - PLoS ONE (2012)

Responses of drought tolerant and sensitive varieties of soybean towards short-term water stress.(a–d) trifoliate leaves and (e–h) nodules. (a, e) dry weight (DW); (b) relative water content (RWC), (f) nitrogenase activity, (c, g) ureide levels and (d, h) proline levels in 17-day-old plants subjected to 10 days water sufficient and deficient conditions. TC: Tolerant genotype Control; TD: Tolerant subjected to Drought, SC: Sensitive genotype Control; SD: Sensitive subjected to Drought. Data represent the mean ± standard deviation (SD) of five to 15 replicates. Different letters at the top of each bar indicate significant differences at P<0.01.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3369847&req=5

pone-0038554-g001: Responses of drought tolerant and sensitive varieties of soybean towards short-term water stress.(a–d) trifoliate leaves and (e–h) nodules. (a, e) dry weight (DW); (b) relative water content (RWC), (f) nitrogenase activity, (c, g) ureide levels and (d, h) proline levels in 17-day-old plants subjected to 10 days water sufficient and deficient conditions. TC: Tolerant genotype Control; TD: Tolerant subjected to Drought, SC: Sensitive genotype Control; SD: Sensitive subjected to Drought. Data represent the mean ± standard deviation (SD) of five to 15 replicates. Different letters at the top of each bar indicate significant differences at P<0.01.
Mentions: The two soybean cultivars used in the present study were categorized at CIAP-INTA (Centro de Investigaciones Agropecuarias – Instituto Nacional de Tecnología Agropecuaria, Argentina) as tolerant (NA5009RG) and sensitive (DM50048) to drought stress based on their ability to maintain relative water content (RWC) and growth, and withstand oxidative stress through the modulation of cellular malondialdehide (MDA) levels. The drought tolerant line maintained higher RWC and showed greater ability to withstand oxidative damage owing to lower production of MDA, and exhibited sustained growth at reduced soil moisture conditions (Dr. Celina Luna, personal communication). In the present study, a comparison of these two soybean drought tolerant and sensitive genotypes was undertaken to determine the differences in their metabolic profiles/responses during water stress. Drought was imposed on nodulated soybean plants 17 days after inoculation by withholding water during 10 days, and physiological characteristics such as dry weight (DW), relative water content (RWC), chlorophyll and nitrogenase activity (ARA), and metabolite profiles were analyzed in order to establish the effect of water stress on these plant parameters (Figure 1). The results of the study showed that the water stress produced varied effects on leaf and nodule metabolism in both drought tolerant and sensitive soybean varieties. Under the drought condition imposed, both RWC and DW of the leaves showed a reduction, more remarkably in the sensitive variety (a decrease of about 10% in RWC and 42% in DW in the sensitive genotype as compared to 9% and 15%, respectively, in the tolerant one; Figure 1a, b). In addition, water stress affected proline and ureide contents in leaves of the sensitive variety but not in the tolerant genotype (Figure 1c, d). On the other hand, no effect of drought stress was observed on chlorophyll content as well as chlorophyll a/b ratio in both the genotypes (data not shown).

Bottom Line: Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil.The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity.Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México. silvente@ccg.unam.mx

ABSTRACT
Soybean (Glycine max L.) is an important source of protein for human and animal nutrition, as well as a major source of vegetable oil. The soybean crop requires adequate water all through its growth period to attain its yield potential, and the lack of soil moisture at critical stages of growth profoundly impacts the productivity. In this study, utilizing (1)H NMR-based metabolite analysis combined with the physiological studies we assessed the effects of short-term water stress on overall growth, nitrogen fixation, ureide and proline dynamics, as well as metabolic changes in drought tolerant (NA5009RG) and sensitive (DM50048) genotypes of soybean in order to elucidate metabolite adjustments in relation to the physiological responses in the nitrogen-fixing plants towards water limitation. The results of our analysis demonstrated critical differences in physiological responses between these two genotypes, and identified the metabolic pathways that are affected by short-term water limitation in soybean plants. Metabolic changes in response to drought conditions highlighted pools of metabolites that play a role in the adjustment of metabolism and physiology of the soybean varieties to meet drought effects.

Show MeSH
Related in: MedlinePlus