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Isolation and characterization of RNA-containing exosomes.

Lässer C, Eldh M, Lötvall J - J Vis Exp (2012)

Bottom Line: This isolation method is based on repeated centrifugation and filtration steps, followed by a final ultracentrifugation step in which the exosomes are pelleted.Important methods to identify the exosomes and characterize the exosomal morphology and protein content are highlighted, including electron microscopy, flow cytometry and Western blot.The purification of the total exosomal RNA is based on spin column chromatography and the exosomal RNA yield and size distribution is analyzed using a Bioanalyzer.

View Article: PubMed Central - PubMed

Affiliation: Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg.

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Related in: MedlinePlus

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Isolation and characterization of RNA-containing exosomes.

Lässer C, Eldh M, Lötvall J - J Vis Exp (2012)

© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3369768&req=5

Bottom Line: This isolation method is based on repeated centrifugation and filtration steps, followed by a final ultracentrifugation step in which the exosomes are pelleted.Important methods to identify the exosomes and characterize the exosomal morphology and protein content are highlighted, including electron microscopy, flow cytometry and Western blot.The purification of the total exosomal RNA is based on spin column chromatography and the exosomal RNA yield and size distribution is analyzed using a Bioanalyzer.

View Article: PubMed Central - PubMed

Affiliation: Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg.

Show MeSH
Related in: MedlinePlus