Isolation and characterization of RNA-containing exosomes.
Bottom Line: This isolation method is based on repeated centrifugation and filtration steps, followed by a final ultracentrifugation step in which the exosomes are pelleted.Important methods to identify the exosomes and characterize the exosomal morphology and protein content are highlighted, including electron microscopy, flow cytometry and Western blot.The purification of the total exosomal RNA is based on spin column chromatography and the exosomal RNA yield and size distribution is analyzed using a Bioanalyzer.
Affiliation: Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg.Show MeSH
Related in: MedlinePlus
Affiliation: Krefting Research Centre, Department of Internal Medicine, Sahlgrenska Academy, University of Gothenburg.