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Entomologic and serologic evidence of zoonotic transmission of Babesia microti, eastern Switzerland.

Foppa IM, Krause PJ, Spielman A, Goethert H, Gern L, Brand B, Telford SR - Emerging Infect. Dis. (2002)

Bottom Line: DNA from pooled nymphal ticks amplified by polymerase chain reaction was highly homologous to published B. microti sequences.In addition, we measured seroprevalence of immunoglobulin (Ig) G antibodies against B. microti antigen in nearby residents.Serum from 1.5% of the 396 human residents of the region reacted to B. microti antigen (>1:64), as determined by indirect immunofluorescence assay (IgG).

View Article: PubMed Central - PubMed

Affiliation: Harvard School of Public Health, Boston, Massachusetts, USA.

ABSTRACT
We evaluated human risk for infection with Babesia microti at a site in eastern Switzerland where several B. microti-infected nymphal Ixodes ricinus ticks had been found. DNA from pooled nymphal ticks amplified by polymerase chain reaction was highly homologous to published B. microti sequences. More ticks carried babesial infection in the lower portion of the rectangular 0.7-ha grid than in the upper (11% vs. 0.8%). In addition, we measured seroprevalence of immunoglobulin (Ig) G antibodies against B. microti antigen in nearby residents. Serum from 1.5% of the 396 human residents of the region reacted to B. microti antigen (>1:64), as determined by indirect immunofluorescence assay (IgG). These observations constitute the first report demonstrating B. microti in a human-biting vector, associated with evidence of human exposure to this agent in a European site.

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Related in: MedlinePlus

Schematic drawing of the sampling scheme. Distance between sampling points is 30 m. The letters and solid arrows denote the sections used for prevalence estimation. Sampling lines that connect points not belonging to a section are not included in that section (e.g., line B4–C2 is not part of section B).
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Figure 1: Schematic drawing of the sampling scheme. Distance between sampling points is 30 m. The letters and solid arrows denote the sections used for prevalence estimation. Sampling lines that connect points not belonging to a section are not included in that section (e.g., line B4–C2 is not part of section B).

Mentions: To assess local prevalence of B. microti in host-seeking nymphal I. ricinus ticks, we developed a tick-sampling procedure with high spatial resolution (Figure 1). The roughly rectangular, 0.7-ha field site, Ruetiwis (9° 38´ E, 46° 59´ N), is located on a steep southwesterly slope near Seewis in the lower Praettigau Valley of eastern Switzerland at an approximate mean altitude of 850 m above sea level. The site is characterized by abandoned pastures that are partly overgrown by young stands of deciduous and coniferous trees and bushes, as well as by mature mixed forest. All ticks were collected by flagging in July 1997.


Entomologic and serologic evidence of zoonotic transmission of Babesia microti, eastern Switzerland.

Foppa IM, Krause PJ, Spielman A, Goethert H, Gern L, Brand B, Telford SR - Emerging Infect. Dis. (2002)

Schematic drawing of the sampling scheme. Distance between sampling points is 30 m. The letters and solid arrows denote the sections used for prevalence estimation. Sampling lines that connect points not belonging to a section are not included in that section (e.g., line B4–C2 is not part of section B).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369589&req=5

Figure 1: Schematic drawing of the sampling scheme. Distance between sampling points is 30 m. The letters and solid arrows denote the sections used for prevalence estimation. Sampling lines that connect points not belonging to a section are not included in that section (e.g., line B4–C2 is not part of section B).
Mentions: To assess local prevalence of B. microti in host-seeking nymphal I. ricinus ticks, we developed a tick-sampling procedure with high spatial resolution (Figure 1). The roughly rectangular, 0.7-ha field site, Ruetiwis (9° 38´ E, 46° 59´ N), is located on a steep southwesterly slope near Seewis in the lower Praettigau Valley of eastern Switzerland at an approximate mean altitude of 850 m above sea level. The site is characterized by abandoned pastures that are partly overgrown by young stands of deciduous and coniferous trees and bushes, as well as by mature mixed forest. All ticks were collected by flagging in July 1997.

Bottom Line: DNA from pooled nymphal ticks amplified by polymerase chain reaction was highly homologous to published B. microti sequences.In addition, we measured seroprevalence of immunoglobulin (Ig) G antibodies against B. microti antigen in nearby residents.Serum from 1.5% of the 396 human residents of the region reacted to B. microti antigen (>1:64), as determined by indirect immunofluorescence assay (IgG).

View Article: PubMed Central - PubMed

Affiliation: Harvard School of Public Health, Boston, Massachusetts, USA.

ABSTRACT
We evaluated human risk for infection with Babesia microti at a site in eastern Switzerland where several B. microti-infected nymphal Ixodes ricinus ticks had been found. DNA from pooled nymphal ticks amplified by polymerase chain reaction was highly homologous to published B. microti sequences. More ticks carried babesial infection in the lower portion of the rectangular 0.7-ha grid than in the upper (11% vs. 0.8%). In addition, we measured seroprevalence of immunoglobulin (Ig) G antibodies against B. microti antigen in nearby residents. Serum from 1.5% of the 396 human residents of the region reacted to B. microti antigen (>1:64), as determined by indirect immunofluorescence assay (IgG). These observations constitute the first report demonstrating B. microti in a human-biting vector, associated with evidence of human exposure to this agent in a European site.

Show MeSH
Related in: MedlinePlus