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Excretion of vancomycin-resistant enterococci by wild mammals.

Mallon DJ, Corkill JE, Hazel SM, Wilson JS, French NP, Bennett M, Hart CA - Emerging Infect. Dis. (2002)

Bottom Line: A survey of fecal samples found enterococcal excretion in 82% of 388 bank voles (Clethrionomys glareolus), 92% of 131 woodmice (Apodemus sylvaticus), and 75% of 165 badgers (Meles meles).Vancomycin-resistant enterococci, all Enterococcus faecium of vanA genotype, were excreted by 4.6% of the woodmice and 1.2% of the badgers, but by none of the bank voles.

View Article: PubMed Central - PubMed

Affiliation: University of Liverpool, Liverpool, United Kingdom.

ABSTRACT
A survey of fecal samples found enterococcal excretion in 82% of 388 bank voles (Clethrionomys glareolus), 92% of 131 woodmice (Apodemus sylvaticus), and 75% of 165 badgers (Meles meles). Vancomycin-resistant enterococci, all Enterococcus faecium of vanA genotype, were excreted by 4.6% of the woodmice and 1.2% of the badgers, but by none of the bank voles.

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Polymerase chain reaction analysis of vancomycin-resistant enterococci isolates for glycopeptide resistance genotypes and species identification.
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Related In: Results  -  Collection


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Figure 1: Polymerase chain reaction analysis of vancomycin-resistant enterococci isolates for glycopeptide resistance genotypes and species identification.

Mentions: VRE were isolated from 6 (5.1%) of 118 woodmice samples from the woodland area only. Two (1.2%) of the badger samples yielded VRE. None of the bank voles at either site were excreting VRE. Seven of the eight VRE were obtained on direct culture on enterococcosel agar. The remaining VRE (from a woodmouse) was obtained from broth culture, as were each of the other VRE also isolated by direct culture. Each of the VREs had vancomycin MICs >64 mg/L and teicoplanin MICs 0.75–6.0 mg/L (Table). Biochemical identification with the API 20 Strep did not provide accurate species assignment. Each VRE had the vanA gene but none of the other resistance genes, and each was confirmed as E. faecium by polymerase chain reaction (Figure). PFGE analysis showed that six of the isolates had band patterns differing by >10 bands, but two woodmice isolates were closely related, differing by only two bands.


Excretion of vancomycin-resistant enterococci by wild mammals.

Mallon DJ, Corkill JE, Hazel SM, Wilson JS, French NP, Bennett M, Hart CA - Emerging Infect. Dis. (2002)

Polymerase chain reaction analysis of vancomycin-resistant enterococci isolates for glycopeptide resistance genotypes and species identification.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369587&req=5

Figure 1: Polymerase chain reaction analysis of vancomycin-resistant enterococci isolates for glycopeptide resistance genotypes and species identification.
Mentions: VRE were isolated from 6 (5.1%) of 118 woodmice samples from the woodland area only. Two (1.2%) of the badger samples yielded VRE. None of the bank voles at either site were excreting VRE. Seven of the eight VRE were obtained on direct culture on enterococcosel agar. The remaining VRE (from a woodmouse) was obtained from broth culture, as were each of the other VRE also isolated by direct culture. Each of the VREs had vancomycin MICs >64 mg/L and teicoplanin MICs 0.75–6.0 mg/L (Table). Biochemical identification with the API 20 Strep did not provide accurate species assignment. Each VRE had the vanA gene but none of the other resistance genes, and each was confirmed as E. faecium by polymerase chain reaction (Figure). PFGE analysis showed that six of the isolates had band patterns differing by >10 bands, but two woodmice isolates were closely related, differing by only two bands.

Bottom Line: A survey of fecal samples found enterococcal excretion in 82% of 388 bank voles (Clethrionomys glareolus), 92% of 131 woodmice (Apodemus sylvaticus), and 75% of 165 badgers (Meles meles).Vancomycin-resistant enterococci, all Enterococcus faecium of vanA genotype, were excreted by 4.6% of the woodmice and 1.2% of the badgers, but by none of the bank voles.

View Article: PubMed Central - PubMed

Affiliation: University of Liverpool, Liverpool, United Kingdom.

ABSTRACT
A survey of fecal samples found enterococcal excretion in 82% of 388 bank voles (Clethrionomys glareolus), 92% of 131 woodmice (Apodemus sylvaticus), and 75% of 165 badgers (Meles meles). Vancomycin-resistant enterococci, all Enterococcus faecium of vanA genotype, were excreted by 4.6% of the woodmice and 1.2% of the badgers, but by none of the bank voles.

Show MeSH