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Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus

The effect of Formula 14 on NSCLC cell lines H460 and H520. Survival rate of H460 (a) and H520 (b) cells following treatment with Formulae14 for 72 hr was analyzed by MTT assay. (c) Formula 14 decreased the colony formation activity of H460 cells. Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (d) Western blots of the cell cycle regulatory proteins in H520 cells after 72 hr treatment of Formula 14. Results of 3 independent experiments were shown at the same time to show the consistent results.
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fig6: The effect of Formula 14 on NSCLC cell lines H460 and H520. Survival rate of H460 (a) and H520 (b) cells following treatment with Formulae14 for 72 hr was analyzed by MTT assay. (c) Formula 14 decreased the colony formation activity of H460 cells. Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (d) Western blots of the cell cycle regulatory proteins in H520 cells after 72 hr treatment of Formula 14. Results of 3 independent experiments were shown at the same time to show the consistent results.

Mentions: To examine whether Formula 14 has similar cytotoxicity toward other NSCLC cells, we further applied Formula on H460 (large cell carcinoma) and H520 (squamous cell carcinoma) cell lines. Our results showed that Formula 14 also reduced the proliferation of both H460 and H520 cells (Figures 6(a) and 6(b)). The IC50 for H460 and H520 are 4.5% and 3.8%, respectively. In addition, Formula 14 also reduced the colony-forming activity of H460 cell line. The number of colonies at 14-day H460 culture was significantly reduced in cells pretreated with Formula 14 compared to that of the control group (Figure 6(c)). The Western blot analyses showed that Formula 14 decreased the expression levels of cell cycle regulatory proteins, CDK4, pRb, and cyclin B1 of H520 cell line (Figure 6(d)). These data showed that Formula 14 has a general effect on different NSCLC cell lines.


Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

The effect of Formula 14 on NSCLC cell lines H460 and H520. Survival rate of H460 (a) and H520 (b) cells following treatment with Formulae14 for 72 hr was analyzed by MTT assay. (c) Formula 14 decreased the colony formation activity of H460 cells. Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (d) Western blots of the cell cycle regulatory proteins in H520 cells after 72 hr treatment of Formula 14. Results of 3 independent experiments were shown at the same time to show the consistent results.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369556&req=5

fig6: The effect of Formula 14 on NSCLC cell lines H460 and H520. Survival rate of H460 (a) and H520 (b) cells following treatment with Formulae14 for 72 hr was analyzed by MTT assay. (c) Formula 14 decreased the colony formation activity of H460 cells. Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (d) Western blots of the cell cycle regulatory proteins in H520 cells after 72 hr treatment of Formula 14. Results of 3 independent experiments were shown at the same time to show the consistent results.
Mentions: To examine whether Formula 14 has similar cytotoxicity toward other NSCLC cells, we further applied Formula on H460 (large cell carcinoma) and H520 (squamous cell carcinoma) cell lines. Our results showed that Formula 14 also reduced the proliferation of both H460 and H520 cells (Figures 6(a) and 6(b)). The IC50 for H460 and H520 are 4.5% and 3.8%, respectively. In addition, Formula 14 also reduced the colony-forming activity of H460 cell line. The number of colonies at 14-day H460 culture was significantly reduced in cells pretreated with Formula 14 compared to that of the control group (Figure 6(c)). The Western blot analyses showed that Formula 14 decreased the expression levels of cell cycle regulatory proteins, CDK4, pRb, and cyclin B1 of H520 cell line (Figure 6(d)). These data showed that Formula 14 has a general effect on different NSCLC cell lines.

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus