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Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus

CHM Formula 14 led to A549 cells arrested in G0/G1 and G2 phase. Cell cycle analysis of A549 cells after treatment with Formula 14. (a) Representative data of cell cycle distribution profile under Formula 14 treatment. Treatment with Formula 14 led to significant cell cycle arrest in G1 and G2 phase. (b) Quantified result of cells at different phases from (a). (c) Representative data of ki67 analysis of A549 cells after a 72 hr treatment of Formula 14. (d) Fraction of cells in G0 and other phases. Formula 14 significantly increased the G0 cell population after 72 hr treatment. Student's t-test: *, **, ***P < 0.05, 0.01, and 0.001, respectively.
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fig4: CHM Formula 14 led to A549 cells arrested in G0/G1 and G2 phase. Cell cycle analysis of A549 cells after treatment with Formula 14. (a) Representative data of cell cycle distribution profile under Formula 14 treatment. Treatment with Formula 14 led to significant cell cycle arrest in G1 and G2 phase. (b) Quantified result of cells at different phases from (a). (c) Representative data of ki67 analysis of A549 cells after a 72 hr treatment of Formula 14. (d) Fraction of cells in G0 and other phases. Formula 14 significantly increased the G0 cell population after 72 hr treatment. Student's t-test: *, **, ***P < 0.05, 0.01, and 0.001, respectively.

Mentions: We then tested whether Formula 14 affected cell cycle progression, and the results showed that the number of cells in the proliferative S phase was significantly decreased whereas cell populations in G1 and G2 phases were significantly increased after 24 and 72 hr treatment, respectively (Figures 4(a) and 4(b)). However, there was no cell population identified in the subG1 phase (Figure 4(a)). These data indicate that CHM Formula 14 treatment did not induce cell apoptosis but rather reduced the proliferation rate of A549 cells. In addition, a Ki67 analysis showed there were more cells in the quiescent G0 phase after treatment with Formula 14, compared to that of the control group (Figures 4(c) and 4(d)). In conclusion, CHM Formula 14 retarded cell cycle progression significantly.


Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

CHM Formula 14 led to A549 cells arrested in G0/G1 and G2 phase. Cell cycle analysis of A549 cells after treatment with Formula 14. (a) Representative data of cell cycle distribution profile under Formula 14 treatment. Treatment with Formula 14 led to significant cell cycle arrest in G1 and G2 phase. (b) Quantified result of cells at different phases from (a). (c) Representative data of ki67 analysis of A549 cells after a 72 hr treatment of Formula 14. (d) Fraction of cells in G0 and other phases. Formula 14 significantly increased the G0 cell population after 72 hr treatment. Student's t-test: *, **, ***P < 0.05, 0.01, and 0.001, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3369556&req=5

fig4: CHM Formula 14 led to A549 cells arrested in G0/G1 and G2 phase. Cell cycle analysis of A549 cells after treatment with Formula 14. (a) Representative data of cell cycle distribution profile under Formula 14 treatment. Treatment with Formula 14 led to significant cell cycle arrest in G1 and G2 phase. (b) Quantified result of cells at different phases from (a). (c) Representative data of ki67 analysis of A549 cells after a 72 hr treatment of Formula 14. (d) Fraction of cells in G0 and other phases. Formula 14 significantly increased the G0 cell population after 72 hr treatment. Student's t-test: *, **, ***P < 0.05, 0.01, and 0.001, respectively.
Mentions: We then tested whether Formula 14 affected cell cycle progression, and the results showed that the number of cells in the proliferative S phase was significantly decreased whereas cell populations in G1 and G2 phases were significantly increased after 24 and 72 hr treatment, respectively (Figures 4(a) and 4(b)). However, there was no cell population identified in the subG1 phase (Figure 4(a)). These data indicate that CHM Formula 14 treatment did not induce cell apoptosis but rather reduced the proliferation rate of A549 cells. In addition, a Ki67 analysis showed there were more cells in the quiescent G0 phase after treatment with Formula 14, compared to that of the control group (Figures 4(c) and 4(d)). In conclusion, CHM Formula 14 retarded cell cycle progression significantly.

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus