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Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus

CHM Formula 14 decreased the colony formation activity of A549 cells. (a) Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (b) Anchorage-independent growth ability. Colony numbers were significantly decreased by treatment with Formula 14 for 72 hr. Student's t-test: *, ***P < 0.05 and 0.001, respectively.
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fig3: CHM Formula 14 decreased the colony formation activity of A549 cells. (a) Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (b) Anchorage-independent growth ability. Colony numbers were significantly decreased by treatment with Formula 14 for 72 hr. Student's t-test: *, ***P < 0.05 and 0.001, respectively.

Mentions: We also conducted colony forming-assay to determine whether Formula 14 could affect the tumorigenesis ability of A549 cells, and the results showed that the number of colonies at 14-day culture was significantly reduced in cells pretreated with Formula 14 compared to that of the control group (Figures 3(a) and 3(b)). In addition, the formation of colonies on soft agar after one month was also dramatically decreased in the Formula 14-treated cells (Figure 3(c)). These two different assays revealed that Formula 14 could reduce tumorigenesis in A549 cells.


Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

CHM Formula 14 decreased the colony formation activity of A549 cells. (a) Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (b) Anchorage-independent growth ability. Colony numbers were significantly decreased by treatment with Formula 14 for 72 hr. Student's t-test: *, ***P < 0.05 and 0.001, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369556&req=5

fig3: CHM Formula 14 decreased the colony formation activity of A549 cells. (a) Cell colonies were stained with crystal violet and scanned to generate images. Colony numbers of those cells treated with Formula 14 (lower panel) were significantly decreased. (b) Anchorage-independent growth ability. Colony numbers were significantly decreased by treatment with Formula 14 for 72 hr. Student's t-test: *, ***P < 0.05 and 0.001, respectively.
Mentions: We also conducted colony forming-assay to determine whether Formula 14 could affect the tumorigenesis ability of A549 cells, and the results showed that the number of colonies at 14-day culture was significantly reduced in cells pretreated with Formula 14 compared to that of the control group (Figures 3(a) and 3(b)). In addition, the formation of colonies on soft agar after one month was also dramatically decreased in the Formula 14-treated cells (Figure 3(c)). These two different assays revealed that Formula 14 could reduce tumorigenesis in A549 cells.

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus