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Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus

CHM Formula 14 decreased A549 cell survival without apparent apoptosis. A time course MTT assay was used to investigate the response of A549 cells to treatment with Formula 14. (a) Formula 14 treatment significantly inhibited the proliferation of A549 cells at all the time points studied. (b) Bright field images of A549 cells after 72 hr treatment with vehicle or Formula 14. The cell density of A549 cells after treatment with Formula 14 was much lower than the control group. (c) Representative data of annexin V and PI double staining show the apoptosis/necrosis status of A549 cells after 72 hr treatment with Formula 14. (d) Percentages of the double negative live cells (bottom left square) were quantified to compare the healthy population. Formula 14 has no effect on the live cell percentage. Student's t-test:,***P < 0.001.
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fig2: CHM Formula 14 decreased A549 cell survival without apparent apoptosis. A time course MTT assay was used to investigate the response of A549 cells to treatment with Formula 14. (a) Formula 14 treatment significantly inhibited the proliferation of A549 cells at all the time points studied. (b) Bright field images of A549 cells after 72 hr treatment with vehicle or Formula 14. The cell density of A549 cells after treatment with Formula 14 was much lower than the control group. (c) Representative data of annexin V and PI double staining show the apoptosis/necrosis status of A549 cells after 72 hr treatment with Formula 14. (d) Percentages of the double negative live cells (bottom left square) were quantified to compare the healthy population. Formula 14 has no effect on the live cell percentage. Student's t-test:,***P < 0.001.

Mentions: To further elucidate the growth inhibiting mechanisms of Formula 14, the time course of the response of A549 cells to Formula 14 treatment was examined. Formula 14 could significantly decreased the A549 cell numbers after 24 hr treatment (Figure 2(a)), suggesting that Formula 14 could either actually kill the cells or inhibit the proliferation of the cells. The cell density of the Formula 14 treated group was much lower than that of control group; however, few dead cells could be seen under microscope observation (Figure 2(b)).


Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

CHM Formula 14 decreased A549 cell survival without apparent apoptosis. A time course MTT assay was used to investigate the response of A549 cells to treatment with Formula 14. (a) Formula 14 treatment significantly inhibited the proliferation of A549 cells at all the time points studied. (b) Bright field images of A549 cells after 72 hr treatment with vehicle or Formula 14. The cell density of A549 cells after treatment with Formula 14 was much lower than the control group. (c) Representative data of annexin V and PI double staining show the apoptosis/necrosis status of A549 cells after 72 hr treatment with Formula 14. (d) Percentages of the double negative live cells (bottom left square) were quantified to compare the healthy population. Formula 14 has no effect on the live cell percentage. Student's t-test:,***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369556&req=5

fig2: CHM Formula 14 decreased A549 cell survival without apparent apoptosis. A time course MTT assay was used to investigate the response of A549 cells to treatment with Formula 14. (a) Formula 14 treatment significantly inhibited the proliferation of A549 cells at all the time points studied. (b) Bright field images of A549 cells after 72 hr treatment with vehicle or Formula 14. The cell density of A549 cells after treatment with Formula 14 was much lower than the control group. (c) Representative data of annexin V and PI double staining show the apoptosis/necrosis status of A549 cells after 72 hr treatment with Formula 14. (d) Percentages of the double negative live cells (bottom left square) were quantified to compare the healthy population. Formula 14 has no effect on the live cell percentage. Student's t-test:,***P < 0.001.
Mentions: To further elucidate the growth inhibiting mechanisms of Formula 14, the time course of the response of A549 cells to Formula 14 treatment was examined. Formula 14 could significantly decreased the A549 cell numbers after 24 hr treatment (Figure 2(a)), suggesting that Formula 14 could either actually kill the cells or inhibit the proliferation of the cells. The cell density of the Formula 14 treated group was much lower than that of control group; however, few dead cells could be seen under microscope observation (Figure 2(b)).

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus