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Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus

Effect of 8 CHM formulae on the cytotoxicity of A549 cells. Survival rate of A549 and MRC5 cells following treatment at the IC50 with the 8 chosen formulae for 72 hr was analyzed by MTT assay. Data were normalized to control groups. Formulae 3, 5, and 14 decreased the survival rate of A549 cells around 50% without affecting that of MRC5 cells.
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fig1: Effect of 8 CHM formulae on the cytotoxicity of A549 cells. Survival rate of A549 and MRC5 cells following treatment at the IC50 with the 8 chosen formulae for 72 hr was analyzed by MTT assay. Data were normalized to control groups. Formulae 3, 5, and 14 decreased the survival rate of A549 cells around 50% without affecting that of MRC5 cells.

Mentions: To test whether these 8 active formulae have any significant nontarget effect, the normal lung cell line MRC5 was included in the cytotoxicity screening. The eight formulae were used to treat A549 and MRC5 cell lines for 72 hr at their IC50 toward A549. We found that Formulae 3, 5, and 14 reduced the growth rate of A549 cells around 50% without significantly affecting the viability of MRC5 cells (Figure 1). In contrast, the other 5 formulae (4, 6, 8, 9, and 15) showed significant nontarget effects, suppressing the growth rate of MRC5 about 20–70% (Figure 1). Among the three A549-specific formulae, Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further study due to its traditional CHM use as therapy for lung disease.


Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest.

Wang TC, Fang CN, Shen CC, Wei HY, Weng YP, Lin JY, Hsieh-Li HM, Lee CY - Evid Based Complement Alternat Med (2012)

Effect of 8 CHM formulae on the cytotoxicity of A549 cells. Survival rate of A549 and MRC5 cells following treatment at the IC50 with the 8 chosen formulae for 72 hr was analyzed by MTT assay. Data were normalized to control groups. Formulae 3, 5, and 14 decreased the survival rate of A549 cells around 50% without affecting that of MRC5 cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3369556&req=5

fig1: Effect of 8 CHM formulae on the cytotoxicity of A549 cells. Survival rate of A549 and MRC5 cells following treatment at the IC50 with the 8 chosen formulae for 72 hr was analyzed by MTT assay. Data were normalized to control groups. Formulae 3, 5, and 14 decreased the survival rate of A549 cells around 50% without affecting that of MRC5 cells.
Mentions: To test whether these 8 active formulae have any significant nontarget effect, the normal lung cell line MRC5 was included in the cytotoxicity screening. The eight formulae were used to treat A549 and MRC5 cell lines for 72 hr at their IC50 toward A549. We found that Formulae 3, 5, and 14 reduced the growth rate of A549 cells around 50% without significantly affecting the viability of MRC5 cells (Figure 1). In contrast, the other 5 formulae (4, 6, 8, 9, and 15) showed significant nontarget effects, suppressing the growth rate of MRC5 about 20–70% (Figure 1). Among the three A549-specific formulae, Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further study due to its traditional CHM use as therapy for lung disease.

Bottom Line: We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells.Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death.These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Science, National Taiwan Normal University, Taipei 116, Taiwan.

ABSTRACT
Lung cancer has long been one of the most deadly forms of cancer. The majority of lung cancers are of the non-small-cell lung cancer (NSCLC) type. Here we used the non-small-cell lung carcinoma cell line A549 to screen 15 different traditional Chinese herbal medicine (CHM) formulae to explore the possible mechanisms of alternative medicine in lung cancer therapy. We identified three formulae (Formulae 3, 5, and 14) that substantially decreased the survival of A549 cells but did not affect MRC5 normal lung tissue cells. Formula 14, Yang-Dan-Tang, a modified decoction of Ramulus Cinnamomi Cassiae, was chosen for further characterization. Flow cytometry analysis showed that treatment of Formula 14 induced cell cycle arrest in G1 and G2 phase without causing significant cell death. These results were also confirmed by Western blot analysis, with decreased expression of G1/S and G2/M promoting cell cycle machinery including cyclin D3, cyclin B1, CDK4, and CDK6. This study provides further insight into the possible working mechanism of Yang-Dan-Tang in patients.

No MeSH data available.


Related in: MedlinePlus